A TVD Scheme using Roe's Flux and the Ambient Boundary Condition

Details of a second-order accurate TVD scheme using the Roe's Riemann solver is described for the three-dimensional Euler equation. The differential equations are discretized using a finite volume formulation. The ambient boundary condition proposed by us is also explained

Three-Dimensional Carbuncles and Euler Fluxes

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/83630/1/AIAA-2010-1273-957.pd

The Osher Upwind Scheme and its Application to Cosmic Gas Dynamics

Details of the second order Osher scheme for the multi-dimensional Euler equation are presented. The adopted method to attain the second order of accuracy differs from the existing second order formulation by Osher. The present method is easy to be implemented and can be applied to other first order upwind schemes. Two types of numerical integration forms are coded. One is written in the integral form (cell method), and the other is the usual finite-difference form. Both forms work well and can capture strong shocks without any auxiliary artificial damping. The integration form strictly satisfies the flux conservation even on geometrical singular coordinate lines, which inevitably appear in three dimensional calculations with bodies embedded. Hydrodynamic calculations of the interaction between a stellar wind and an accretion flow are performed to demonstrate the ability of the present method

Numerical Simulations of Axisymmetric Flows in Astrophysics and their Visualization by a Video Movie

Numerical simulations of axisymmetric flow in four different astrophysical situations are performed, and their results are visualized by using a video movie. The situations considered are : 1) an accretion flow on to a gravitating compact object allowing maximum accrection ; 2) a jet formation in a flow past a gravitating rigid sphere ; 3) an interaction between a supersonic wind from a central object and a uniform supersonic incident flow ; 4) a wind bubble formed by spherical supersonic wind. The video tape of VHS/NTSC format is available from the author

Characterization of galactose-dependent promoters from an oleaginous fungus Mortierella alpina 1S-4.

An inducible promoter is a useful tool for the controlled expression of a given gene. In this report, we describe galactose-dependent promoters for potential use in an oleaginous fungus Mortierella alpina. We cloned the putative promoter regions of two genes encoding galactose metabolic enzymes, GAL1 and GAL10, from the genome of M. alpina 1S-4. The β-glucuronidase (GUS) reporter gene assay in M. alpina 1S-4 revealed that regulation of these promoters was dependent on the presence of galactose in the medium both with and without other sugars. With the GAL10 promoter, an approximately 50-fold increase of GUS activity was demonstrated by addition of galactose into the culture media at any cultivation phase. The 5' deletion analysis of the GAL10 promoter revealed that a promoter region of over 2, 000 bp length was required for its high-level activity and sufficient inducible response. Significantly, this is the first report of inducible promoters of zygomycetes. The GAL10 promoter will be a valuable tool for gene manipulation in M. alpina 1S-4

Selection and characterization of promoters based on genomic approach for the molecular breeding of oleaginous fungus Mortierella alpina 1S-4.

To express a foreign gene effectively, a good expression system is required. In this study, we investigated various promoters as useful tools for gene manipulation in oleaginous fungus Mortierella alpina 1S-4. We selected and cloned the promoter regions of 28 genes in M. alpina 1S-4 on the basis of expression sequence tag abundance data. The activity of each promoter was evaluated using the β-glucuronidase (GUS) reporter gene. Eight of these promoters were shown to enhance GUS expression more efficiently than a histone promoter, which is conventionally used for the gene manipulation in M. alpina. Especially, the predicted protein 3 and the predicted protein 6 promoters demonstrated approximately fivefold higher activity than the histone promoter. The activity of some promoters changed along with the cultivation phase of M. alpina 1S-4. Seven promoters with constitutive or time-dependent, high-level expression activity were selected, and deletion analysis was carried out to determine the promoter regions required to retain activity. This is the first report of comprehensive promoter analysis based on a genomic approach for M. alpina. The promoters described here will be useful tools for gene manipulation in this strain

Metabolic engineering of oleaginous fungus Mortierella alpina for high production of oleic and linoleic acids

The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68.0%. The production of LA and OA in these transformants reached 1.44 and 2.76 g/L, respectively, on the 5th day. The Δ6I transcriptional levels of the RNAi-treated strains were suppressed to 1/10th that in the parent strain. The amount of Δ6II RNA in the Δ6I RNAi-treated strain increased to 8 times that in the wild strain

Evaluation of Euler Fluxes for Hypersonic Heating Computations

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/83575/1/AIAA-41605-439.pd