45 research outputs found
Green- and blue-light-mediated chloroplast migration in the centric diatom Pleurosira laevis
The existence of two photoreceptors regulating chloroplast orientation was found in the centric diatom Pleurosira laevis. Chloroplasts migrate through the transvacuolar cytoplasmic strands according to the light conditions. Weak white light of less than 46 μmol/m2 · s (10 W/m2) induces chloroplast movement to the cortical cytoplasm, which is located next to the plasma membrane (dispersion), while intense white light of more than 92 μmol/m2 · s (20 W/m2) induces chloroplast movement towards the nucleus, which is situated in the center of the cell (assemblage). Chloroplast dispersion was maintained as long as the cells were irradiated with weak white light. Conversely, chloroplast assemblage under intense white light was transient and the chloroplasts were released from assemblage after 15 min. Action spectra determined with the Okazaki Large Spectrograph revealed that the weak white light receptor and the intense white light receptor are characterized by 540 nm and 450 nm optima, respectively
Distinct responses of chloroplasts to blue and green laser microbeam irradiations in the centric diatom Pleurosira laevis
The centric diatomPleurosira laevis is a large unicellular alga, in which ca 200 chloroplasts migrate toward the nuclear cytoplasm through the transvacuolar cytoplasmic strands in response to blue-light irradiation and, on the contrary, toward the cortical cytoplasm in response to green-light irradiation. We analyzed these light-induced chloroplast migrations using a scanning laser microbeam provided by a confocal microscope for intracellular irradiation. Spot irradiation of a blue laser microbeam induced rapid assemblage of chroloplasts into the nuclear cytoplasm regardless of the spot position and spot number. On the other hand, one or two spots of green laser microbeam induced chloroplast accumulation at the spots, although increasing spot numbers suppressed chloroplast accumulation at each spot. In our experimental condition, ca 1 min of blue-light irradiation was sufficient to stimulate movement, whereas green-light irradiation required uninterrupted and longer irradiation time (ca 15 min). Chloroplast assemblage induced by blue-light required extracellular Ca 2+, and was inhibited by Ca 2+channel antagonists. Furthermore, higher efficiencies of chloroplast migration were obtained when a single beam spot was fragmented and scattered over wider area of plasma membrane. These observations suggested that blue-light induced a response at the plasma membrane, which subsequently activated Ca 2+permeable channels. This sequence of physiological events is identical to what was previously observed with chloroplast movement in response to mechanical stimulation. Furthermore, experiments with the cytoskeleton-disrupting agents, colchicine and cytochalasin D, indicated that blue-light-induced chloroplast movement required microtubules whereas the green-light-induced response to beam spot required actin filaments