Comparison between New-Onset and Old-Diagnosed Type 2 Diabetes with Ketosis in Rural Regions of China

Objectives. Type 2 diabetes (T2D) with ketosis was common because of late diagnosis and lacking adequate treatment in rural regions of China. This study aimed to provide the data of T2D with ketosis among inpatients in a south-west border city of China. Methods. Data of 371 patients of T2D with ketosis who were hospitalized between January 2011 and July 2015 in Baoshan People’s Hospital, Yunnan, China, were analyzed. New-onset and old-diagnosed T2D patients presenting with ketosis were compared according to clinical characteristics, laboratory results, and chronic diabetic complications. Results. Overall, the blood glucose control was poor in our study subjects. Male predominated in both groups (male prevalence was 68% in new-onset and 64% in old-diagnosed groups). Overweight and obesity accounted for 50% in new-onset and 46% in old-diagnosed cases. Inducements of ketosis were 13.8% in new-onset and 38.7% in old-diagnosed patients. Infections were the first inducements in both groups. The prevalence of chronic complications of diabetes was common in both groups. Conclusions. More medical supports were needed for the early detection and adequate treatment of diabetes in rural areas of China

A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts

Terminally differentiated cells can be generated by lineage reprogramming, which is, however, hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming.</p

Human Hepatocytes with Drug Metabolic Function Induced from Fibroblasts by Lineage Reprogramming

SummaryObtaining fully functional cell types is a major challenge for drug discovery and regenerative medicine. Currently, a fundamental solution to this key problem is still lacking. Here, we show that functional human induced hepatocytes (hiHeps) can be generated from fibroblasts by overexpressing the hepatic fate conversion factors HNF1A, HNF4A, and HNF6 along with the maturation factors ATF5, PROX1, and CEBPA. hiHeps express a spectrum of phase I and II drug-metabolizing enzymes and phase III drug transporters. Importantly, the metabolic activities of CYP3A4, CYP1A2, CYP2B6, CYP2C9, and CYP2C19 are comparable between hiHeps and freshly isolated primary human hepatocytes. Transplanted hiHeps repopulate up to 30% of the livers of Tet-uPA/Rag2−/−/γc−/− mice and secrete more than 300 μg/ml human ALBUMIN in vivo. Our data demonstrate that human hepatocytes with drug metabolic function can be generated by lineage reprogramming, thus providing a cell resource for pharmaceutical applications

Long-term functional maintenance of primary human hepatocytes in vitro

The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening.</p

The Impact of Rural Population Aging on Farmers’ Cleaner Production Behavior: Evidence from Five Provinces of the North China Plain

The purpose of this article is to examine the impact of rural population aging on farmers’ cleaner production behavior through the intervening effects of three aspects (learning capacity, factor substitution and behavior imitation). The data used in this article were collected from a questionnaire survey of 916 farmers in the main grain producing provinces of the North China Plain. First, confirmatory factor analysis was carried out, and then data were analyzed through structural equation modeling using the bootstrap methodology in AMOS 24. The findings indicate that rural population aging inhibits farmers’ cleaner production behavior through learning capacity. In contrast, rural population aging promotes farmers’ cleaner production behavior through factor substitution and behavior imitation. From the perspective of both challenges and opportunities, this article investigates the impact mechanism and comprehensive effect of rural population aging on farmers’ cleaner production behavior, so as to explore new ways to promote cleaner agricultural production under the background of an accelerated aging the of rural labor force and provide reference for the formulation of relevant policies

The Impact of Village Rules and Formal Environmental Regulations on Farmers’ Cleaner Production Behavior: New Evidence from China

Village rules and formal environmental regulations are of great significance for standardizing farmers’ cleaner production behavior, promoting green transformation of agriculture and realizing sustainable development of agriculture. Based on the survey data of 946 farmers in five provinces of China, taking seed coating technology, soil testing and formulated fertilization technology, subsoiling tillage technology, green technology for pest and disease control and straw returning technology as examples, this article empirically analyzes the impact of village rules and formal environmental regulations on farmers’ cleaner production behavior by using the multivariate probit model. When formal environmental regulations are relatively lacking or weak, village rules can be used as a useful supplement to formal environmental regulations to promote farmers’ participation in cleaner production. Based on this, this article argues that the important reason for formal environmental regulations falling into relative system failure is that village rules have not been paid enough attention in promoting farmers’ cleaner production behavior. In the future, we should not only continue to strengthen the role of formal environmental regulations in farmers’ cleaner production, but also cultivate the informal institution represented by the village rules, and build the regulatory system of mutual support between informal institution and formal institution

Comparison between New-Onset and Old-Diagnosed Type 2 Diabetes with Ketosis in Rural Regions of China

Objectives. Type 2 diabetes (T2D) with ketosis was common because of late diagnosis and lacking adequate treatment in rural regions of China. This study aimed to provide the data of T2D with ketosis among inpatients in a south-west border city of China. Methods. Data of 371 patients of T2D with ketosis who were hospitalized between January 2011 and July 2015 in Baoshan People&apos;s Hospital, Yunnan, China, were analyzed. New-onset and old-diagnosed T2D patients presenting with ketosis were compared according to clinical characteristics, laboratory results, and chronic diabetic complications. Results. Overall, the blood glucose control was poor in our study subjects. Male predominated in both groups (male prevalence was 68% in new-onset and 64% in old-diagnosed groups). Overweight and obesity accounted for 50% in new-onset and 46% in old-diagnosed cases. Inducements of ketosis were 13.8% in new-onset and 38.7% in old-diagnosed patients. Infections were the first inducements in both groups. The prevalence of chronic complications of diabetes was common in both groups. Conclusions. More medical supports were needed for the early detection and adequate treatment of diabetes in rural areas of China

Does Fish Oil Have an Anti-Obesity Effect in Overweight/Obese Adults? A Meta-Analysis of Randomized Controlled Trials

<div><p>Context</p><p>Accumulating evidence has suggested favorable effects of fish oil on weight loss in animal experiments; however, findings remain inconsistent in humans.</p><p>Objects</p><p>The meta-analysis was performed to investigate the influence of fish oil on some parameters of body composition in overweight/obese adults.</p><p>Design</p><p>Human randomized, placebo-controlled trials were identified by a systematic search of Embase, PubMed, the Cochrane Library, web of science and reference lists of related reviews and articles. The random-effects model was used to estimate the calculated results.</p><p>Results</p><p>In total, 21 studies with 30 study arms were included in this analysis. Calculated results of the meta-analysis demonstrated that fish oil had no effect on reducing body weight (overall SMD = -0.07, 95% CI -0.21 to 0.07, <i>P</i> = 0.31) and BMI (overall SMD = -0.09, 95% CI -0.22 to 0.03, <i>P</i> = 0.14) whether alone or combined with life modification intervention in overweight/obese subjects. However, waist circumference was significantly reduced (SMD = -0.23, 95% CI -0.40 to -0.06, <i>P</i> = 0.008) in those with fish oil supplementation combined with life modification intervention. Waist hip ratio (WHR) was significantly reduced (overall SMD = -0.52 95% CI -0.76 to -0.27, <i>P</i> < 0.0005) in fish oil supplemented individuals with or without combination life modification intervention.</p><p>Conclusion</p><p>Current evidence cannot support an exact anti-obesity role of n-3 polyunsaturated fatty acids (PUFAs) in overweight/obese subjects. However, these subjects may benefit from reducing abdominal fat with fish oil supplementation especially when combined with life modification intervention. Further large-scale and long-term clinical trials are needed to gain definite conclusions.</p></div

Forest plots from meta-analyses for the effects of fish oil on changes in body weight (A), BMI (B), waist circumference (C), and WHR (D).

<p>Forest plots from meta-analyses for the effects of fish oil on changes in body weight (A), BMI (B), waist circumference (C), and WHR (D).</p

Subgroup estimation of the effects of fish oil on body weight changes.

<p>Subgroup estimation of the effects of fish oil on body weight changes.</p