2,823 research outputs found
Lack of Methylene Blue Staining in Superficial Epithelia as a Possible Marker for Superficial Lateral Spread of Bile Duct Cancer
Longitudinal cancer spread is very important for staging of resectability in bile duct cancer. We verified the difference in methylene blue staining properties between cancerous and noncancerous epithelia that are usually observed by cholangioscopy. We obtained 45 biopsy specimens from the common bile duct of 20 patients with bile duct disease using percutaneous transhepatic cholangioscopy (PTCS) after staining with 0.05% methylene blue. We compared the microscopic staining properties with the gross endoscopic observations and evaluated the characteristics of methylene blue staining on frozen sections of each type of cholangial epithelium. Microscopic staining properties were significantly associated with endoscopic observations (p = 0.00001). While 18 of 20 (90%) specimens of normal epithelia stained with methylene blue, 11 of 16 (69%) specimens of metaplastic epithelia were stained, with no staining obtained in cancerous
epithelia. The cancerous epithelia stained significantly less often than either the normal (p = 0.000005) or the metaplastic (p = 0.001) epithelia. Evaluation of methylene blue staining during PTCS revealed that this stain was absorbed by the cholangial epithelia, not superficially stuck to it. The difference in methylene blue staining properties between the cancerous and normal epithelia could be helpful to clarify the boundary of superficial lateral spread of bile duct cancer
Produksi alkaloid kuinina oleh beberapa Mikroba endofit dengan penambahan zat induser (Studi Mikroba Endofit Tanaman Cinchona sp. (2)): Production of quinine alkaloid by some endophytic microbes with addition of inducer
Mikroba endofit diketahui berpotensi sebagai sumber senyawa aktif untuk bahan obat-obatan. Optimisasi medium fermentasi termasuk dengan cara penambahan zat induser diperlukan untuk mendapatkan hasil produksi yang optimum. Oleh karena itu, penelitian ini dilakukan untuk mengetahui pengaruh penam bahan serbuk ka:yu tanaman Cinchona succirubra terhadap produksi senyawa kuinina oleh mikroba endofit yang ditumbuhkan dalam dua jenis medium cair yang berbeda.
Mikroba endofit yang diisolasi dari tanaman Cinchona sp. yang diinkitbasilcan - dalam medium Potato Dextrose Broth (PDB) dan medium Phoma yang telah ditambah serbuk kayu C. succirubra selama 3 hari pada mesin.penggojog (shaker) dan produksi kuinina dianalisis dengan kromatografi cair Idnerja tinggi (KCKT).
Hasil menunjukkan bahwa ada peningkatan produksi kuinina yang bervariasi untuk tiap jenis mikroba. Peningkatan produksi tertinggi sebesar 2,2 kali lipat dicapai oleh isolat kapang J3P1 yang ditumbuhkan dalam medium PDB.
Kata kunci zat induserkuininamikroba endofitCinchona succirubra
ABSTRACT
Endophytic microbes have been. known to be potential as the sources of active compound for medicines. Optimization of medium, including of inducer addition, is necessary to obtain optimum products. This study, therefore, was aimed to investigate the effect of Cinchona succirubra bark powder addition in the production of quinine compound by endophytic microbe growingin two different liquid media.
Endophytic microbe isolated from Cinchona sp. were shaker incubated in Potato Dextrose Broth (PDB) and Phoma media with the addition of bark powder of C. succirubra for 3 days. Quinine production was analyzed with High Performance Liquid Chromatography (HPLC).
The result showed an increasing of quinine production for each microbes. Highest increasing of quinine production (212 folds) was yielded by mold culture (J3P1) grown in PDB medium.
Keywords: inducer, quinine, endophytic microbe, Cinchona succirubr
A multimedia data visualization based on Ad Hoc communication networks and its application to disaster management
After massive earthquakes and other large-scale disasters, existing communication infrastructure may become unavailable and, therefore, it can be quite difficult for relief organizations to fully grasp the impact of the disaster on the affected region. Consequently, this will be the cause of delays to offer the strategic assistance, and to provide water and food, etc. In order to solve the problem of re-establishing communication infrastructure to allow for information gathering, we developed an ad hoc mobile communications network for disaster-struck areas using ZigBee. As the communication speed of ZigBee is low, we propose a problem-specific image compression method for the multimedia data visualization. By using the proposed method combined with GPS information, it is possible to quickly grasp the damage situation in the region. Through our communication experiments in Tsukuba City, Japan we confirm the effectiveness of our system as a disaster information gathering and management system
Indexing k-mers in linear space for quality value compression.
Many bioinformatics tools heavily rely on [Formula: see text]-mer dictionaries to describe the composition of sequences and allow for faster reference-free algorithms or look-ups. Unfortunately, naive [Formula: see text]-mer dictionaries are very memory-inefficient, requiring very large amount of storage space to save each [Formula: see text]-mer. This problem is generally worsened by the necessity of an index for fast queries. In this work, we discuss how to build an indexed linear reference containing a set of input [Formula: see text]-mers and its application to the compression of quality scores in FASTQ files. Most of the entropies of sequencing data lie in the quality scores, and thus they are difficult to compress. Here, we present an application to improve the compressibility of quality values while preserving the information for SNP calling. We show how a dictionary of significant [Formula: see text]-mers, obtained from SNP databases and multiple genomes, can be indexed in linear space and used to improve the compression of quality value. Availability: The software is freely available at https://github.com/yhhshb/yalff
Effects of local hypothermia-rewarming on physiology, metabolism and inflammation of acutely injured human spinal cord.
In five patients with acute, severe thoracic traumatic spinal cord injuries (TSCIs), American spinal injuries association Impairment Scale (AIS) grades A-C, we induced cord hypothermia (33 °C) then rewarming (37 °C). A pressure probe and a microdialysis catheter were placed intradurally at the injury site to monitor intraspinal pressure (ISP), spinal cord perfusion pressure (SCPP), tissue metabolism and inflammation. Cord hypothermia-rewarming, applied to awake patients, did not cause discomfort or neurological deterioration. Cooling did not affect cord physiology (ISP, SCPP), but markedly altered cord metabolism (increased glucose, lactate, lactate/pyruvate ratio (LPR), glutamate; decreased glycerol) and markedly reduced cord inflammation (reduced IL1β, IL8, MCP, MIP1α, MIP1β). Compared with pre-cooling baseline, rewarming was associated with significantly worse cord physiology (increased ICP, decreased SCPP), cord metabolism (increased lactate, LPR; decreased glucose, glycerol) and cord inflammation (increased IL1β, IL8, IL4, IL10, MCP, MIP1α). The study was terminated because three patients developed delayed wound infections. At 18-months, two patients improved and three stayed the same. We conclude that, after TSCI, hypothermia is potentially beneficial by reducing cord inflammation, though after rewarming these benefits are lost due to increases in cord swelling, ischemia and inflammation. We thus urge caution when using hypothermia-rewarming therapeutically in TSCI
Brachytherapy of stage II mobile tongue carcinoma. Prediction of local control and QOL
BACKGROUND: There is no consensus as to the prognostic model for brachytherapy of tongue carcinoma. This study was designed to evaluate the prognostic factors for local control based on a large population under a unified treatment policy. RESULTS: Between 1970 and 1998, 433 patients with stage II tongue squamous cell carcinoma were treated by low-dose-rate brachytherapy. This series included 277 patients treated with a linear source with a minimum follow-up of 3 years. A spacer was introduced in 1987. The primary local control rates were 85.6%. CONCLUSION: In the multivariate analysis, an invasive growth pattern was a significant factor for local recurrence. The disease-related survival was influenced by old age and an invasive growth pattern. A spacer lowered mandibular bone complications. The growth pattern was the most important factor for recurrence. Brachytherapy was associated with a high cure rate and the use of spacers brought about good quality of life (QOL)
Functional Relations in Stokes Multipliers and Solvable Models related to U_q(A^{(1)}_n)
Recently, Dorey and Tateo have investigated functional relations among Stokes
multipliers for a Schr{\"o}dinger equation (second order differential equation)
with a polynomial potential term in view of solvable models. Here we extend
their studies to a restricted case of n+1-th order linear differential
equations.Comment: 20 pages, some explanations improved, To appear in J. Phys.
Internal states of model isotropic granular packings. III. Elastic properties
In this third and final paper of a series, elastic properties of numerically
simulated isotropic packings of spherical beads assembled by different
procedures and subjected to a varying confining pressure P are investigated. In
addition P, which determines the stiffness of contacts by Hertz's law, elastic
moduli are chiefly sensitive to the coordination number, the possible values of
which are not necessarily correlated with the density. Comparisons of numerical
and experimental results for glass beads in the 10kPa-10MPa range reveal
similar differences between dry samples compacted by vibrations and lubricated
packings. The greater stiffness of the latter, in spite of their lower density,
can hence be attributed to a larger coordination number. Voigt and Reuss bounds
bracket bulk modulus B accurately, but simple estimation schemes fail for shear
modulus G, especially in poorly coordinated configurations under low P.
Tenuous, fragile networks respond differently to changes in load direction, as
compared to load intensity. The shear modulus, in poorly coordinated packings,
tends to vary proportionally to the degree of force indeterminacy per unit
volume. The elastic range extends to small strain intervals, in agreement with
experimental observations. The origins of nonelastic response are discussed. We
conclude that elastic moduli provide access to mechanically important
information about coordination numbers, which escape direct measurement
techniques, and indicate further perspectives.Comment: Published in Physical Review E 25 page
Designing isolation guidelines for COVID-19 patients with rapid antigen tests
新型コロナウイルス感染者の隔離短縮は可能か?. 京都大学プレスリリース. 2022-08-24.Appropriate isolation guidelines for COVID-19 patients are warranted. Currently, isolating for fixed time is adopted in most countries. However, given the variability in viral dynamics between patients, some patients may no longer be infectious by the end of isolation, whereas others may still be infectious. Utilizing viral test results to determine isolation length would minimize both the risk of prematurely ending isolation of infectious patients and the unnecessary individual burden of redundant isolation of noninfectious patients. In this study, we develop a data-driven computational framework to compute the population-level risk and the burden of different isolation guidelines with rapid antigen tests (i.e., lateral flow tests). Here, we show that when the detection limit is higher than the infectiousness threshold values, additional consecutive negative results are needed to ascertain infectiousness status. Further, rapid antigen tests should be designed to have lower detection limits than infectiousness threshold values to minimize the length of prolonged isolation
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