Heterologous Expression of ATG8c from Soybean Confers Tolerance to Nitrogen Deficiency and Increases Yield in Arabidopsis

Nitrogen is an essential element for plant growth and yield. Improving Nitrogen Use Efficiency (NUE) of crops could potentially reduce the application of chemical fertilizer and alleviate environmental damage. To identify new NUE genes is therefore an important task in molecular breeding. Macroautophagy (autophagy) is an intracellular process in which damaged or obsolete cytoplasmic components are encapsulated in double membraned vesicles termed autophagosomes, then delivered to the vacuole for degradation and nutrient recycling. One of the core components of autophagosome formation, ATG8, has been shown to directly mediate autophagosome expansion, and the transcript of which is highly inducible upon starvation. Therefore, we postulated that certain homologs of Saccharomyces cerevisiae ATG8 (ScATG8) from crop species could have potential for NUE crop breeding. A soybean (Glycine max, cv. Zhonghuang-13) ATG8, GmATG8c, was selected from the 11 family members based on transcript analysis upon nitrogen deprivation. GmATG8c could partially complement the yeast atg8 mutant. Constitutive expression of GmATG8c in soybean callus cells not only enhanced nitrogen starvation tolerance of the cells but accelerated the growth of the calli. Transgenic Arabidopsis over-expressing GmATG8c performed better under extended nitrogen and carbon starvation conditions. Meanwhile, under optimum growth conditions, the transgenic plants grew faster, bolted earlier, produced larger primary and axillary inflorescences, eventually produced more seeds than the wild-type. In average, the yield was improved by 12.9%. We conclude that GmATG8c may serve as an excellent candidate for breeding crops with enhanced NUE and better yield

Beyond “Political” Communicative Spaces: Talking Politics on the Wife Swap Discussion Forum

Net-based public sphere researchers have examined online deliberation in numerous ways. However, most studies have focused exclusively on political discussion forums. This article moves beyond such spaces by analyzing political talk from an online forum dedicated to reality television. The purpose is to examine the democratic quality of political talk that emerges in this space in light of a set of normative criteria of the public sphere. The analysis also moved beyond an elite model of deliberation by investigating the use of expressives (humor, emotional comments, and acknowledgments). The findings reveal that participants engaged in political talk that was often deliberative. It was a space where the use of expressives played a significant role in enhancing such talk

Symbol Equivalence in Phonetics

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The Phantom Schwa: A Pseudo Symbol

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Phonetic Simultaneity

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Unvoiced Aspirated vs. Voiced Unaspirated Polar Plosives: A Critique of Some Theories

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