Development and application of a simple LC-MS method for the determination of plasma rilpivirine (TMC-278) concentrations

Rilpivirine (TMC-278) is a second-generation non-nucleoside reverse transcriptase inhibitor that is high potent against both wild-type and drug-resistant HIV-1 strains. Therefore, rilpivirine is expected to treat therapy-experienced patients who failed to use current drugs due to the emergence of drug-resistant HIV mutants. The quantification of rilpivirine in human plasma is important to support clinical studies and determine pharmacokinetic parameters of rilpivirine in HIV-1 infected patients. Consequently, simple and easy system to determine plasma rilpivirine concentrations has been required. In this study, we developed a conventional LC-MS method to quantify plasma rilpivirine. Subsequently the method was validated by estimating the precision and accuracy for inter- and intraday analysis in the concentration range of 18-715 ng/ml. The calibration curve was linear in this range. Average accuracy ranged from 100.0 to 100.6%. Relative standard deviations of both inter- and intraday assays were less than 3.3%. Recovery of rilpivirine was more than 82.0%. These results demonstrate that our LC-MS method provides a conventional, accurate and precise way to determine rilpivirine in human plasma. This method can be used in routine clinical application for HIV-1 infected patients, and permits management of drug interactions and toxicity for rilpivirine

Histone variant H2A.B-H2B dimers are spontaneously exchanged with canonical H2A-H2B in the nucleosome

精子形成に重要なヒストンによるDNAの新たな折りたたみを解明. 京都大学プレスリリース. 2021-02-22.H2A.B is an evolutionarily distant histone H2A variant that accumulates on DNA repair sites, DNA replication sites, and actively transcribing regions in genomes. In cells, H2A.B exchanges rapidly in chromatin, but the mechanism has remained enigmatic. In the present study, we found that the H2A.B-H2B dimer incorporated within the nucleosome exchanges with the canonical H2A-H2B dimer without assistance from additional factors, such as histone chaperones and nucleosome remodelers. High-speed atomic force microscopy revealed that the H2A.B nucleosome, but not the canonical H2A nucleosome, transiently forms an intermediate “open conformation”, in which two H2A.B-H2B dimers may be detached from the H3-H4 tetramer and bind to the DNA regions near the entry/exit sites. Mutational analyses revealed that the H2A.B C-terminal region is responsible for the adoption of the open conformation and the H2A.B-H2B exchange in the nucleosome. These findings provide mechanistic insights into the histone exchange of the H2A.B nucleosome

A manual sequence method of peptides and phosphopeptides using 4-(1\u27-cyanoisoindolyl)phenylisothiocyanate.

A method for sequence analysis and identification of phosphoamino acids in peptides based on high performance liquid chromatography (HPLC) is described. The peptides were derivatized with an Edman type reagent, 4-(1\u27-cyanoisoindolyl)phenylisothiocyanate (CIPIC) and subsequently cleaved to generate stable and fluorescent 4-(1\u27-cyanoisoindolyl)phenylthiazolinone (CIP-TZ)-amino acids. Several experimental factors that affected derivatization on membranes were examined. Under the optimized conditions, the CIP-TZ derivatives of Try(p), Thr(p) and Ser(p) were obtained and separated from their parent amino acids with baseline resolution using an isocratic elution system. Up to the 4th residue of phosphorylated pentapeptides was successfully identified, whereas phosphoamino acid residues could not be detected by the conventional procedure using phenylisothiocyanate (PITC). The results demonstrated the potential of CIPIC as a derivatization reagent for peptide sequencing and the applicability of the method for the study and identification of phosphoamino acids in peptides

Spectra of V1405 Cas at the very beginning indicate a low-mass ONeMg white dwarf progenitor

The lowest possible mass of ONeMg white dwarfs (WDs) has not been clarified despite its importance in the formation and evolution of WDs. We tackle this issue by studying the properties of V1405 Cas (Nova Cassiopeiae 2021), which is an outlier given a combination of its very slow light-curve evolution and the recently reported neon-nova identification. We report its rapid spectral evolution in the initial phase, covering 9.88, 23.77, 33.94, 53.53, 71.79, and 81.90 hours after the discovery. The first spectrum is characterized by lines from highly-ionized species, most noticeably He II and N III. These lines are quickly replaced by lower-ionization lines, e.g., N II, Si II, and O I. In addition, Al II (6237 \r{A}) starts emerging as an emission line at the second epoch. We perform emission-line strength diagnostics, showing that the density and temperature quickly decrease toward later epochs. This behavior, together with the decreasing velocity seen in H$\alpha$, H$\beta$, and He I, indicates that the initial nova dynamics is reasonably well described by an expanding fireball on top of an expanding photosphere. Interestingly, the strengths of the N III and Al II indicate large abundance enhancement, pointing to an ONeMg WD progenitor as is consistent with its neon-nova classification. Given its low-mass nature inferred by the slow light-curve evolution and relatively narrow emission lines, it provides a challenge to the stellar evolution theory that predicts the lower limit of the ONeMg WD mass being $\sim$ 1.1 $M_\odot$.Comment: 15 pages, 5 figures, 2 tables, accepted to Ap