A Multivariate Surface-Based Analysis of the Putamen in Premature Newborns: Regional Differences within the Ventral Striatum

Many children born preterm exhibit frontal executive dysfunction, behavioral problems including attentional deficit/hyperactivity disorder and attention related learning disabilities. Anomalies in regional specificity of cortico-striato-thalamo-cortical circuits may underlie deficits in these disorders. Nonspecific volumetric deficits of striatal structures have been documented in these subjects, but little is known about surface deformation in these structures. For the first time, here we found regional surface morphological differences in the preterm neonatal ventral striatum. We performed regional group comparisons of the surface anatomy of the striatum (putamen and globus pallidus) between 17 preterm and 19 term-born neonates at term-equivalent age. We reconstructed striatal surfaces from manually segmented brain magnetic resonance images and analyzed them using our in-house conformal mapping program. All surfaces were registered to a template with a new surface fluid registration method. Vertex-based statistical comparisons between the two groups were performed via four methods: univariate and multivariate tensor-based morphometry, the commonly used medial axis distance, and a combination of the last two statistics. We found statistically significant differences in regional morphology between the two groups that are consistent across statistics, but more extensive for multivariate measures. Differences were localized to the ventral aspect of the striatum. In particular, we found abnormalities in the preterm anterior/inferior putamen, which is interconnected with the medial orbital/prefrontal cortex and the midline thalamic nuclei including the medial dorsal nucleus and pulvinar. These findings support the hypothesis that the ventral striatum is vulnerable, within the cortico-stiato-thalamo-cortical neural circuitry, which may underlie the risk for long-term development of frontal executive dysfunction, attention deficit hyperactivity disorder and attention-related learning disabilities in preterm neonates. © 2013 Shi et al

Sulforaphane induces adipocyte browning and promotes glucose and lipid utilization

Scope: Obesity is closely related to the imbalance of white adipose tissue storing excess calories, and brown adipose tissue dissipating energy to produce heat in mammals. Recent studies revealed that acquisition of brown characteristics by white adipocytes, termed “browning,” may positively contribute to cellular bioenergetics and metabolism homeostasis. The goal was to investigate the putative effects of natural antioxidant sulforaphane (1-isothiocyanate-4-methyl-sulfonyl butane; SFN) on browning of white adipocytes. Methods and Results: 3T3-L1 mature white adipocytes were treated with SFN for 48 h, and then the mitochondrial content, function, and energy utilization were assessed. SFN was found to induce 3T3-L1 adipocytes browning based on the increased mitochondrial content and activity of respiratory chain enzymes, whereas the mechanism involved the upregulation of nuclear factor E2-related factor 2/ sirtuin1/ peroxisome proliferator-activated receptor gamma coactivator 1 alpha signaling. SFN enhanced uncoupling protein 1 expression, a marker for brown adipocyte, leading to the decrease in cellular ATP. SFN also enhanced glucose uptake and oxidative utilization, lipolysis and fatty acid oxidation in 3T3-L1 adipocytes. Conclusion: SFN-induced browning of white adipocytes enhanced the utilization of cellular fuel, and the application of SFN is a promising strategy to combat obesity and obesity-related metabolic disorder

NaMemo: Enhancing Lecturers' Interpersonal Competence of Remembering Students' Names

Addressing students by their names helps a teacher to start building rapport with students and thus facilitates their classroom participation. However, this basic yet effective skill has become rather challenging for university lecturers, who have to handle large-sized (sometimes exceeding 100) groups in their daily teaching. To enhance lecturers' competence in delivering interpersonal interaction, we developed NaMemo, a real-time name-indicating system based on a dedicated face-recognition pipeline. This paper presents the system design, the pilot feasibility test, and our plan for the following study, which aims to evaluate NaMemo's impacts on learning and teaching, as well as to probe design implications including privacy considerations.Comment: DIS '20 Companio

Internal Flow Phenomena of Two-Way Contra-Rotating Axial Flow Pump-Turbine in Pump Mode under Variable Speed

This paper investigated the variable speed operation of a two-way contra-rotating axial flow pump–turbine in pump mode. When counter-rotating impellers operate at the same speed, a significant difference exists in the shaft power of the two impellers, thereby causing difficulties in motor selection. However, the same motor is required for both impellers in two-way pump–turbines. To solve this problem, this paper aims to determine the appropriate speed at which the two impellers operate with a similar shaft power. Accordingly, improving the performance of the tidal unit while enabling the basic functions of the pump–turbine is of considerable engineering and academic significance. An analysis of performance variations of the front and rear impellers in the pump mode reveals the law of power variation for each impeller at different speeds of the rear impeller. Under different flow conditions, shaft power of the rear impeller is found to be at least 31% higher compared with that of the front impeller. This result provides practical reference for further research on the operation of counter-rotating axial flow pump–turbine

Fractal atomic-level percolation in metallic glasses

Metallic glasses are metallic alloys that exhibit exotic material properties. They may have fractal structures at the atomic level, but a physical mechanism for their organization without ordering has not been identified. We demonstrated a crossover between fractal short-range (<2 atomic diameters) and homogeneous long-range structures using in situ x-ray diffraction, tomography, and molecular dynamics simulations. A specific class of fractal, the percolation cluster, explains the structural details for several metallic-glass compositions. We postulate that atoms percolate in the liquid phase and that the percolating cluster becomes rigid at the glass transition temperature

Novel cyclic di-GMP effectors of the YajQ protein family control bacterial virulence

Bis-(3 ',5 ') cyclic di-guanylate (cyclic di-GMP) is a key bacterial second messenger that is implicated in the regulation of many critical processes that include motility, biofilm formation and virulence. Cyclic di-GMP influences diverse functions through interaction with a range of effectors. Our knowledge of these effectors and their different regulatory actions is far from complete, however. Here we have used an affinity pull-down assay using cyclic di-GMP-coupled magnetic beads to identify cyclic di-GMP binding proteins in the plant pathogen Xanthomonas campestris pv. campestris (Xcc). This analysis identified XC_3703, a protein of the YajQ family, as a potential cyclic di-GMP receptor. Isothermal titration calorimetry showed that the purified XC_3703 protein bound cyclic di-GMP with a high affinity (K-d similar to 2 mu M). Mutation of XC_3703 led to reduced virulence of Xcc to plants and alteration in biofilm formation. Yeast two-hybrid and far-western analyses showed that XC_3703 was able to interact with XC_2801, a transcription factor of the LysR family. Mutation of XC_2801 and XC_3703 had partially overlapping effects on the transcriptome of Xcc, and both affected virulence. Electromobility shift assays showed that XC_3703 positively affected the binding of XC_2801 to the promoters of target virulence genes, an effect that was reversed by cyclic di-GMP. Genetic and functional analysis of YajQ family members from the human pathogens Pseudomonas aeruginosa and Stenotrophomonas maltophilia showed that they also specifically bound cyclic di-GMP and contributed to virulence in model systems. The findings thus identify a new class of cyclic di-GMP effector that regulates bacterial virulence

Microphase Separation Induced by Differential Interactions in Diblock Copolymer/Homopolymer Blends

Phase behavior of diblock copolymer/homopolymer blends (AB/C) is investigated theoretically. The study focuses on a special case where all three binary pairs, A/B, B/C and C/A, are miscible. Despite the miscibility of the binary pairs, a closed-loop immiscible region exists in the AB/C blends when the A/C and B/C pair interactions are sufficiently different. Inside the closed-loop, the system undergoes microphase separation, exhibiting different ordered structures. This phenomenon is enhanced when the homopolymer (C) interacts more strongly to one of the blocks (A or B).Comment: 19 pages, 7 figures, submitted to J. Chem. Phy

Dynamic transition in driven vortices across the peak effect in superconductors

We study the zero-temperature dynamic transition from the disordered flow to an ordered flow state in driven vortices in type-II superconductors. The transition current $I_{p}$ is marked by a sharp kink in the $V(I)$ characteristic with a concomitant large increase in the defect concentration. On increasing magnetic field $B$, the $I_{p}(B)$ follows the behaviour of the critical current $I_{c}(B)$. Specifically, in the peak effect regime $I_{p}(B)$ increases rapidly along with $I_{c}$. We also discuss the effect of varying disorder strength on $I_{p}$.Comment: 4 pages, 4 figure

Strong quantum fluctuation of vortices in the new superconductor MgB2MgB_2

By using transport and magnetic measurement, the upper critical field $H_{c2}(T)$ and the irreversibility line $H_{irr}(T)$ has been determined. A big separation between $H_{c2}(0)$ and $H_{irr}(0)$ has been found showing the existence of a quantum vortex liquid state induced by quantum fluctuation of vortices in the new superconductor $MgB_2$. Further investigation on the magnetic relaxation shows that both the quantum tunneling and the thermally activated flux creep weakly depends on temperature. But when the melting field $H_{irr}$ is approached, a drastic rising of the relaxation rate is observed. This may imply that the melting of the vortex matter at a finite temperature is also induced by the quantum fluctuation of vortices.Comment: 4 pages, 4 figure