Quantitative proteomic analysis of sphere-forming stem-like oral cancer cells.

IntroductionThe purpose of this study is to identify target proteins that may play important functional roles in oral cancer stem-like cells (CSCs) using mass spectrometry-based quantitative proteomics.MethodsSphere-formation assays were performed on highly invasive UM1 and lowly invasive UM2 oral cancer cell lines, which were derived from the same tongue squamous cell carcinoma, to enrich CSCs. Quantitative proteomic analysis of CSC-like and non-CSC UM1 cells was carried out using tandem mass tagging and two-dimensional liquid chromatography with Orbitrap mass spectrometry.ResultsCSC-like cancer cells were found to be present in the highly invasive UM1 cell line but absent in the lowly invasive UM2 cell line. Stem cell markers SOX2, OCT4, SOX9 and CD44 were up-regulated, whereas HIF-1 alpha and PGK-1 were down-regulated in CSC-like UM1 cells versus non-CSC UM1 cells. Quantitative proteomic analysis indicated that many proteins in cell cycle, metabolism, G protein signal transduction, translational elongation, development, and RNA splicing pathways were differentially expressed between the two cell phenotypes. Both CREB-1-binding protein (CBP) and phosphorylated CREB-1 were found to be significantly over-expressed in CSC-like UM1 cells.ConclusionsCSC-like cells can be enriched from the highly invasive UM1 oral cancer cell line but not from the lowly invasive UM2 oral cancer cell line. There are significant proteomic alterations between CSC-like and non-CSC UM1 cells. In particular, CBP and phosphorylated CREB-1 were significantly up-regulated in CSC-like UM1 cells versus non-CSC UM1 cells, suggesting that the CREB pathway is activated in the CSC-like cells

OpinSummEval: Revisiting Automated Evaluation for Opinion Summarization

Opinion summarization sets itself apart from other types of summarization tasks due to its distinctive focus on aspects and sentiments. Although certain automated evaluation methods like ROUGE have gained popularity, we have found them to be unreliable measures for assessing the quality of opinion summaries. In this paper, we present OpinSummEval, a dataset comprising human judgments and outputs from 14 opinion summarization models. We further explore the correlation between 24 automatic metrics and human ratings across four dimensions. Our findings indicate that metrics based on neural networks generally outperform non-neural ones. However, even metrics built on powerful backbones, such as BART and GPT-3/3.5, do not consistently correlate well across all dimensions, highlighting the need for advancements in automated evaluation methods for opinion summarization. The code and data are publicly available at https://github.com/A-Chicharito-S/OpinSummEval/tree/main.Comment: preprint, included 2 more metrics compared with the previous submissio

Quantum Communication Network Utilizing Quadripartite Entangled States of Optical Field

We propose two types of quantum dense coding communication networks with optical continuous variables, in which a quadripartite entangled state of the optical field with totally three-party correlations of quadrature amplitudes is utilized. In the networks, the exchange of information between any two participants can be manipulated by one or two of the remaining participants. The channel capacities for a variety of communication protocols are numerically calculated. Due to the fact that the quadripartite entangled states applied in the communication systems have been successfully prepared already in the laboratory, the proposed schemes are experimentally accessible at present

Syntenin-1 is a promoter and prognostic marker of head and neck squamous cell carcinoma invasion and metastasis.

Metastasis represents a key factor associated with poor prognosis of head and neck squamous cell carcinoma (HNSC). However, the underlying molecular mechanisms remain largely unknown. In this study, our liquid chromatography with tandem mass spectrometry analysis revealed a number of significantly differentially expressed membrane/membrane-associated proteins between high invasive UM1 and low invasive UM2 cells. One of the identified membrane proteins, Syntenin-1, was remarkably up-regulated in HNSC tissues and cell lines when compared to the controls, and also over-expressed in recurrent HNSC and high invasive UM1 cells. Syntenin-1 over-expression was found to be significantly associated with lymph node metastasis and disease recurrence. HNSC patients with higher syntenin-1 expression had significantly poorer long term overall survival and similar results were found in many other types of cancers based on analysis of The Cancer Genome Atlas data. Finally, knockdown of syntenin-1 inhibited the proliferation, migration and invasion of HNSC cells, and opposite findings were observed when syntenin-1 was over-expressed. Collectively, our studies indicate that syntenin-1 promotes invasion and progression of HNSC. It may serve as a valuable biomarker for lymph node metastasis or a potential target for therapeutic intervention in HNSC

Studies on Nano-Indentation of Polymeric Thin Films Using Finite Element Methods

In this paper, the numerical simulation for nano-indentation is performed to measure time-dependent behavior of polymeric films. The possibility to extract the relaxed shear modulus of the polymer is evaluated using a rigid ball indenter. The viscoelastic behavior of the polymer was represented by the standard model. The effects of Poisson’s ratio are also discussed.Singapore-MIT Alliance (SMA

DialogXL: All-in-One XLNet for Multi-Party Conversation Emotion Recognition

This paper presents our pioneering effort for emotion recognition in conversation (ERC) with pre-trained language models. Unlike regular documents, conversational utterances appear alternately from different parties and are usually organized as hierarchical structures in previous work. Such structures are not conducive to the application of pre-trained language models such as XLNet. To address this issue, we propose an all-in-one XLNet model, namely DialogXL, with enhanced memory to store longer historical context and dialog-aware self-attention to deal with the multi-party structures. Specifically, we first modify the recurrence mechanism of XLNet from segment-level to utterance-level in order to better model the conversational data. Second, we introduce dialog-aware self-attention in replacement of the vanilla self-attention in XLNet to capture useful intra- and inter-speaker dependencies. Extensive experiments are conducted on four ERC benchmarks with mainstream models presented for comparison. The experimental results show that the proposed model outperforms the baselines on all the datasets. Several other experiments such as ablation study and error analysis are also conducted and the results confirm the role of the critical modules of DialogXL.Comment: Accepted by AAAI 2021 main conferenc

Quantitative proteomic analysis of sphere-forming stem-like oral cancer cells

INTRODUCTION: The purpose of this study is to identify target proteins that may play important functional roles in oral cancer stem-like cells (CSCs) using mass spectrometry-based quantitative proteomics. METHODS: Sphere-formation assays were performed on highly invasive UM1 and lowly invasive UM2 oral cancer cell lines, which were derived from the same tongue squamous cell carcinoma, to enrich CSCs. Quantitative proteomic analysis of CSC-like and non-CSC UM1 cells was carried out using tandem mass tagging and two-dimensional liquid chromatography with Orbitrap mass spectrometry. RESULTS: CSC-like cancer cells were found to be present in the highly invasive UM1 cell line but absent in the lowly invasive UM2 cell line. Stem cell markers SOX2, OCT4, SOX9 and CD44 were up-regulated, whereas HIF-1 alpha and PGK-1 were down-regulated in CSC-like UM1 cells versus non-CSC UM1 cells. Quantitative proteomic analysis indicated that many proteins in cell cycle, metabolism, G protein signal transduction, translational elongation, development, and RNA splicing pathways were differentially expressed between the two cell phenotypes. Both CREB-1-binding protein (CBP) and phosphorylated CREB-1 were found to be significantly over-expressed in CSC-like UM1 cells. CONCLUSIONS: CSC-like cells can be enriched from the highly invasive UM1 oral cancer cell line but not from the lowly invasive UM2 oral cancer cell line. There are significant proteomic alterations between CSC-like and non-CSC UM1 cells. In particular, CBP and phosphorylated CREB-1 were significantly up-regulated in CSC-like UM1 cells versus non-CSC UM1 cells, suggesting that the CREB pathway is activated in the CSC-like cells