29 research outputs found
The interaction between XBP1 and eNOS contributes to endothelial cell migration
Trabalho final de mestrado integrado em Medicina ĂĄrea cientĂfica de Urologia, apresentado ĂĄ Faculdade de Medicina da Universidade de CoimbraINTRODUĂĂO: Nas Ășltimas dĂ©cadas a nefrectomia parcial tem assumido um papel de
destaque no tratamento do carcinoma de células renais. Esta técnica, também denominada
cirurgia poupadora de nefrĂłnios, permite remover o tumor na totalidade, preservando o
parĂȘnquima renal adjacente. As suas indicaçÔes tornaram-se mais abrangentes Ă medida que
os resultados se mostraram cada vez mais promissores. Assim, actualmente a nefrectomia
parcial é o tratamento padrão do carcinoma de células renais, em detrimento da tradicional
nefrectomia radical. Discute-se agora qual das duas, nefrectomia parcial aberta ou
laparoscópica, é a melhor opção.
OBJECTIVOS: Rever as evidĂȘncias cientĂficas relativas ao tratamento do carcinoma de
células renais pela nefrectomia parcial, aberta e laparoscópica, definindo o papel actual de
cada uma delas nesta ĂĄrea.
MĂTODOS: Pesquisa na PubMed e Medline de artigos relativos a nefrectomia radical e
nefrectomia parcial aberta e laparoscĂłpica, publicados entre 1997 e 2009. Consulta das
âGuidelinesâ da Associação Europeia de Urologia.
DESENVOLVIMENTO: A nefrectomia parcial mostra resultados oncolĂłgicos
semelhantes aos da nefrectomia radical, permitindo uma menor morbilidade renal a longo
prazo. A nefrectomia parcial aberta Ă© agora o tratamento recomendado pelas âGuidelinesâ da
Associação Europeia de Urologia para o tratamento de tumores com diùmetro inferior a 4 cm,
limitados ao rim, mesmo quando o rim contralateral é normal. As indicaçÔes poderão ainda
ser alargadas para tumores atĂ© 7 cm de diĂąmetro, dependendo das caracterĂsticas do tumor. A
nefrectomia parcial laparoscópica, embora tecnicamente mais exigente, é uma boa opção de
tratamento em centros com experiĂȘncia na realização da tĂ©cnica, revelando bons resultados a
curto prazo.
CONCLUSĂES: A nefrectomia parcial aberta Ă© actualmente o tratamento padrĂŁo do
carcinoma de células renais. A nefrectomia parcial laparoscópica revela resultados a curto
prazo semelhantes aos da nefrectomia parcial aberta, contudo sĂŁo necessĂĄrios estudos
confirmando esses resultados a longo prazoINTRODUCTION: In the last decades partial nephrectomy have had an important role in
the treatment of renal cell carcinoma. This surgery, also known as nephron-sparing surgery,
allows to remove the tumor and to preserve the surrounding healthy renal parenchyma.
Moreover, with the evolution of the technique its indications got more comprehensive. So,
nowadays, partial nephrectomy is the standard treatment for renal cell carcinoma, instead of
the traditional radical nephrectomy. There is now a discussion between which of the two,
open or laparoscopic partial nephrectomy, is the best option.
OBJECTIVES: Review the scientific evidences related to the treatment of renal cell
carcinoma through open and laparoscopic partial nephrectomy, defining the actual role of
both in this area.
METHODS: Research articles related to radical nephrectomy, open and laparoscopic
partial nephrectomies at PubMed and Medline publications between 1997 and 2009. Consult
of the Guidelines of European Urology Association.
DEVELOPMENT: Partial nephrectomy shows oncological outcomes similar to those of
radical nephrectomy with less renal morbidity at long-term follow-up. Open partial
nephrectomy is now the recommended treatment by the Guidelines of European Urology
Association for the treatment of tumors less than 4 cm, limited to the kidney, even when the
other kidney is normal. Indications can also include tumors less than 7 cm, depending on the
characteristics of the tumor. Laparoscopic partial nephrectomy, although technically more
demanding, is a good option in specialized centers and reveals similar results in a short-term.
CONCLUSIONS: Nowadays open partial nephrectomy is the standard treatment of renal
cell carcinoma. Laparoscopic partial shows similar results as open surgery in a short-term,
however, more studies are needed to confirm this results at long-term.
Key-words: Open partial nephrectomy, laparoscopic partial nephrectomy, renal cell
carcinoma, indications, results
Chemical ordering suppresses large-scale electronic phase separation in doped manganites
For strongly correlated oxides, it has been a long-standing issue regarding the role of the chemical ordering of the dopants on the physical properties. Here, using unit cell by unit cell superlattice growth technique, we determine the role of chemical ordering of the Pr dopant in a colossal magnetoresistant (La1-yPry)1-xCaxMnO3 (LPCMO) system, which has been well known for its large length-scale electronic phase separation phenomena. Our experimental results show that the chemical ordering of Pr leads to marked reduction of the length scale of electronic phase separations. Moreover, compared with the conventional Pr-disordered LPCMO system, the Pr-ordered LPCMO system has a metalâinsulator transition that is ~100 K higher because the ferromagnetic metallic phase is more dominant at all temperatures below the Curie temperature
Effects of lysate/tissue storage at â80°C on subsequently extracted EVs of epithelial ovarian cancer tissue origins
Summary: Small extracellular vesicles (sEVs) and large extracellular vesicles (lEVs), play vital roles in intercellular communication. We optimized a method that extracts EVs from epithelial ovarian cancer (EOC) tissues for the purpose of investigating whether cryopreservation of EOC tissues affects the phenotypes, contents, and biological functions of extracted EVs. EV morphology, the number and size distribution of EVs, and EV-related markers were analyzed. Storage of lysates at â80°C decreased lEV yield and increased sEV yield, whereas storage of tissues at â80°C increased both sEV and lEV yields; neither changed the morphology or particle mass ratio of EVs. The two cryopreservation groups retained over 90% of proteins and 80% of miRNAs detected in the âfreshâ group. EVs extracted following lysate/tissue storage at â80°C could also promote angiogenesis and invasive migration ability in human endothelial cells. Cryopreserved EOC tissue may benefit clinical applications for studies of tissue-derived EVs, especially EV proteins-related ones
Synthesis of Benzimidazo[1,2â<i>c</i>]quinazolines via Metal-Free Intramolecular CâH Amination Reaction
A series
of benzimidazoÂ[1,2-<i>c</i>]Âquinazolines have
been synthesized via phenyliodineÂ(III) diacetate (PIDA)-mediated intramolecular
CâH bond cycloamination reaction. This method results in a
direct oxidative CâN bond formation in a complex molecule by
using a metal-free protocol. A plausible reaction mechanism was described
on the basis of the experiments. Some new compounds were evaluated
for their antitumor activity against HUH 7 and SK-HEP-1 hepatocarcinoma
cell line. Among the compounds screened, <b>4m</b> was found
to be the most active compound against HUH 7
RovM and CsrA Negatively Regulate Urease Expression in Yersinia pseudotuberculosis
Urease acts as an important acid resistance system and virulence factor that is widespread among microorganisms. RovM is a global regulator that regulates a series of genes and pathways including acid survival systems in the enteric bacterium Yersinia pseudotuberculosis (Yptb). However, whether RovM regulates the urease activity in Yptb was still unknown. In this study, by using qualitative and quantitative urease assays, we show that the urease expression responds to nutrient conditions and the RovM protein represses urease expression by binding to its promoter. A previously reported positive regulator OmpR activates urease activity but RovM plays a dominant role in different nutrient conditions. In addition, carbon storage regulator system A (CsrA), the upstream regulator of RovM, dramatically down-regulates urease activity possibly by its binding to the Shine-Dalgarno (SD) sequence of the mRNA encoding the urease. In conclusion, this study demonstrates that urease activity is strictly controlled by nutrient conditions and is down-regulated by the CsrA-RovM pathway
Pure Chloride 2D/3D Heterostructure Passivation for Efficient and Stable Perovskite Solar Cells
To date, organicâinorganic hybrid perovskite solar cells (PSCs) have reached a certified efficiency of 25.7%, showing their great potential in industrial commercialization. However, defects at the surface and grain boundaries hinder their device performance and longâterm stability. Herein, longâchain dodecylammonium halides (DACl, DABr, and DAI) to treat the perovskite surface and improve the device performance are introduced. It is found that the three passivators can all form 2D perovskites but with different halide compositions. The DAClâtreated perovskite forms a pure chloride DA2PbCl4 2D perovskite, while the DABr and DAIâtreated surfaces form a pure iodide DA2PbI4 2D perovskite. Compared with the DA2PbI4 layer, it is found that the DA2PbCl4 passivation layer can more effectively passivate defects, improve carrier separation at the perovskite surface, and optimize the energy alignment between the perovskite film and hole transport layer. As a result, a champion power conversion efficiency of 23.91% is achieved for the DAClâtreated PSCs. Moreover, the device maintains around 95% of its initial efficiency after 1000âh storage under relative humidity of 10% at 25â°C
Stem cell-based therapeutic strategies for rotator cuff tendinopathy
Rotator cuff tendinopathy is a common musculoskeletal disorder that imposes significant health and economic burden. Stem cell therapy has brought hope for tendon healing in patients with final stage rotator cuff tendinopathy. Some clinical trials have confirmed the effectiveness of stem cell therapy for rotator cuff tendinopathy, but its application has not been promoted and approved. There are still many issues that should be solved prior to using stem cell therapy in clinical applications. The optimal source and dose of stem cells for rotator cuff tendinopathy should be determined. We also proposed novel prospective approaches that can overcome cell population heterogeneity and standardize patient types for stem cell applications. The translational potential of this article: This review explores the optimal sources of stem cells for rotator cuff tendinopathy and the principles for selecting stem cell dosages. Key strategies are provided for stem cell population standardization and recipient selection
The Type VI Secretion System Engages a Redox-Regulated Dual-Functional Heme Transporter for Zinc Acquisition
The type VI secretion system was recently reported to be involved in zinc acquisition, but the underlying mechanism remains unclear. Here, we report that Burkholderia thailandensis T6SS4 is involved in zinc acquisition via secretion of a zinc-scavenging protein, TseZ, that interacts with the outer membrane heme transporter HmuR. We find that HmuR is a redox-regulated dual-functional transporter that transports heme iron under normal conditions but zinc upon sensing extracellular oxidative stress, triggered by formation of an intramolecular disulfide bond. Acting as the first line of defense against oxidative stress, HmuR not only guarantees an immediate response to the changing environment but also provides a fine-tuned mechanism that allows a gradual response to perceived stress. The T6SS/HmuR-mediated active zinc transport system is also involved in bacterial virulence and contact-independent bacterial competition. We describe a sophisticated bacterial zinc acquisition mechanism affording insights into the role of metal ion transport systems