Efficacy of mannan-oligosaccharide and live yeast feed additives on performance, rumen morphology, serum biochemical parameters and muscle morphometric characteristics in buffalo calves

The objective of the current study was to assess the effect of dietary supplementations of mannan-oligosaccharide, live yeast, and a combination of these two additives on growth performance, histo-morphology of the rumen, and muscle morphometric attributes in buffalo calves. A total of twenty buffalo calves (average weight of 25 kg) having 3 months of age were distributed according to a complete randomized design. All animals were individually stalled in the shed and were fed ad-libitum. Experimental animals were divided into four groups for 67 days: Control group(without the inclusion of dietary supplementation); MOS group (Mannan oligosaccharide 5 g/clave/day; Yeast group (Live yeast 2g/calve/day) and Mixed group (MOS + Live Yeast 2.5g + 1g )/calve/day. Experimental results revealed that combined supplementation of MOS and Yeast and MOS alone resulted in an increased number of short-chain fatty acids in the rumen as well as ruminal pH (P<0.05). Results showed a significant improvement in average daily gain and FCR of MOS and Mixed supplemented groups (P<0.05). Histomorphological evaluation of rumen mucosal epithelium showed a significant improvement in the mixed-supplemented group (P<0.05) as compared to the yeast-supplemented and control groups. Muscle quality parameters such as meat texture showed significant improvement in MOS and mix-supplemented groups. Histological examination of longissimus dorsi muscle cross-section showed a significantly higher(P<0.05) muscle fiber and muscle fascicle diameter in both MOS and mix-supplemented calves groups. In conclusion, the results of this experiment revealed that the dietary addition of MOS, Live yeast, and their combination have positive effects on growth performance, rumen histology indices, and muscle morphometric features in buffalo calves.Comment: Pages 13, 4 figure

Detection of structural mosaicism from targeted and whole-genome sequencing data.

Structural mosaic abnormalities are large post-zygotic mutations present in a subset of cells and have been implicated in developmental disorders and cancer. Such mutations have been conventionally assessed in clinical diagnostics using cytogenetic or microarray testing. Modern disease studies rely heavily on exome sequencing, yet an adequate method for the detection of structural mosaicism using targeted sequencing data is lacking. Here, we present a method, called MrMosaic, to detect structural mosaic abnormalities using deviations in allele fraction and read coverage from next-generation sequencing data. Whole-exome sequencing (WES) and whole-genome sequencing (WGS) simulations were used to calculate detection performance across a range of mosaic event sizes, types, clonalities, and sequencing depths. The tool was applied to 4911 patients with undiagnosed developmental disorders, and 11 events among nine patients were detected. For eight of these 11 events, mosaicism was observed in saliva but not blood, suggesting that assaying blood alone would miss a large fraction, possibly >50%, of mosaic diagnostic chromosomal rearrangements

Xuetonglactones A–F: Highly Oxidized Lanostane and Cycloartane Triterpenoids From Kadsura heteroclita Roxb. Craib

© Copyright © 2020 Shehla, Li, Cao, Zhao, Jian, Daniyal, Wahab, Khan, Liao, Rahman, Choudhary and Wang. Xuetonglactones A–F (1–6), six unreported highly oxidized lanostane- and cycloartane-type triterpenoids along with 22 known scaffolds (7–28) were isolated from the stems of Kadsura heteroclita (Roxb.) Craib. Compared with previous congeners, xuetonglactone A (1), possesses an unprecedented 20,21-α-epoxide, and xuetonglactone D (4) features an unusual 19-α-hydroperoxyl moiety. The structures and the absolute configurations of the compounds were established by extensive one- and two-dimensional NMR, and electronic circular dichroism (ECD) spectroscopic analysis, with those of 1 and 5 confirmed by single-crystal X-ray diffraction technique. Compounds 1 and 2 exhibited inhibition of iNOS activity in LPS-induced macrophages with IC50 values of 22.0, and 17.0 μg/mL, respectively. While compounds 6, 7, 8, and 24 showed potent cytotoxic activities against human cervical cancer cell lines (HeLa) with the IC50 values of 4.0, 5.8, 5.0, and 6.4 μM, and against human gastric cancer cells (BGC 823) with the IC50 values of 2.0, 5.0, 2.5, and 2.0 μM, respectively. Moreover, plausible biogenetic pathways of (1–6) were also proposed

[Letter] A distinct genotype of XP complementation group A: surprisingly mild phenotype highly prevalent in Northern India/ Pakistan/ Afghanistan

No description supplie

Factors associated with patients’ understanding of their management plan in Tshwane clinics

BACKGROUND: This research focused on patients’ views regarding healthcare services and identified factors associated with understanding of their management plan. AIM: To develop a baseline for patient–clinician collaboration and the extent to which patients felt included and understood their treatment plan. SETTING: Tshwane district (South Africa) public health outpatient clinics. METHOD: Medical students interviewed 447 patients in 22 clinics in Tshwane district. Agreement was measured by the percentage of cases in which patients and clinicians were in accord about a particular aspect of the consultation. RESULTS: About one-third of patients incorrectly answered questions on whether changes in lifestyle or diet were prescribed as part of their treatment. The likelihood that patients understood their plan was associated with seeing the same clinician three or more times; having a consultation in their same or a similar language; patient participation in the diagnosis; and feeling that the clinician had explained their health problems to them. CONCLUSIONS: There is need for greater emphasis on continuity of care, the clinicians’ ability to speak the patient’s language and involving patients in the consultation.PRESENTATION: Cette étude se concentrait sur les points de vue des patients concernant les services de santé et les facteurs identifiés associés à la compréhension de leur plan de prise en charge. OBJECTIF: Développer une référence pour la collaboration entre patients et médecins et déterminer la mesure dans laquelle les patients avaient le sentiment d’être inclus à la préparation de leur plan de prise en charge et de le comprendre. CADRE: Centres médicaux publics accueillant des patients en consultation externe dans le district de Tshwane (Afrique du Sud). METHODE: Des étudiants en médecine ont interrogé 447 patients dans 22 centres médicaux dans le district de Tshwane. La concordance était mesurée par le pourcentage de cas dans lesquels les patients et les médecins étaient d’accord sur un aspect particulier de la consultation. RESULTATS: Environ un tiers des patients n’a pas su répondre correctement aux questions visant à déterminer si des changements dans leur style de vie ou régime alimentaire avaient été prescrits dans le cadre de leur traitement. La probabilité que les patients comprennent leur plan était associée au fait de consulter le même médecin à trois reprises ou plus, de consulter dans leur langue ou dans une langue similaire, de participer au diagnostic, et au sentiment que le médecin leur avait expliqué le problème de santé dont ils souffraient. CONCLUSIONS: Il est nécessaire de mettre davantage l’accent sur la continuité de la prise en charge, la capacité des médecins à parler la langue de leurs patients, et l’implication des patients dans la consultation.The authors are indebted to the 2012 class of fifth-year students who took part in this research. We thank them for their commitment to the project as well as their insightful feedback.J.H. (University of Pretoria) was the project leader. J.H., A.R. and T.R. (University of Pretoria) conceptualised and designed the study. L.F., T.R., N.S. (University of Pretoria) and H.K. and S.M. (University of Pretoria and Foundation for Professional Development) were responsible for experimental and questionnaire design. T.M. (University of Pretoria) made significant conceptual contributions to the article. L.F., T.M. and T.R. conducted the analysis and interpretation of the findings and prepared the manuscript. N.S. contributed to data collection and preparation.http://www.phcfm.orgam201

Graphene oxide incorporated polyether sulfone nanocomposite antifouling ultrafiltration membranes with enhanced hydrophilicity

In this study, the polyether sulfone (PES) based membranes containing various concentrations of graphene oxide (GO), polyvinylpyrrolidone (PVP), and polyethylene glycol (PEG) were synthesized via the phase immersion method. This study aims to evaluate the effect of GO addition on the structural properties and performance of the membranes. The membranes were analyzed by x-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier transforms infrared spectroscopy (FTIR). The FTIR-ATR spectra indicated the presence of hydroxyl and carboxylic acid groups on the surface of GO-incorporated membranes, which improved their dispersion in the polymeric matrix and hydrophilicity. The SEM analysis of the GO-containing PES membranes confirmed the formation of a well-defined finger-like porous structure presenting adequate water flux (95 l.m(-2).h(-1)) and salt rejection (72%) compared to the pristine PES membranes (46 l.m(-2).h(-1) and similar to 35%, respectively). In addition, the significantly large wettability and considerably improved antibacterial characteristic (against S. aureus and E. coli strains) of the GO-PES membranes are considered impressive features.National University of Sciences and Technology (NUST) Research Directorate; HEC; NRPU [6020]6020; Higher Education Commission, Pakistan, HEC; National University of Sciences and Technology, NUS

Delineating the molecular and phenotypic spectrum of the SETD1B-related syndrome

Purpose Pathogenic variants in SETD1B have been associated with a syndromic neurodevelopmental disorder including intellectual disability, language delay, and seizures. To date, clinical features have been described for 11 patients with (likely) pathogenic SETD1B sequence variants. This study aims to further delineate the spectrum of the SETD1B-related syndrome based on characterizing an expanded patient cohort. Methods We perform an in-depth clinical characterization of a cohort of 36 unpublished individuals with SETD1B sequence variants, describing their molecular and phenotypic spectrum. Selected variants were functionally tested using in vitro and genome-wide methylation assays. Results Our data present evidence for a loss-of-function mechanism of SETD1B variants, resulting in a core clinical phenotype of global developmental delay, language delay including regression, intellectual disability, autism and other behavioral issues, and variable epilepsy phenotypes. Developmental delay appeared to precede seizure onset, suggesting SETD1B dysfunction impacts physiological neurodevelopment even in the absence of epileptic activity. Males are significantly overrepresented and more severely affected, and we speculate that sex-linked traits could affect susceptibility to penetrance and the clinical spectrum of SETD1B variants. Conclusion Insights from this extensive cohort will facilitate the counseling regarding the molecular and phenotypic landscape of newly diagnosed patients with the SETD1B-related syndrome

Gain-of-Function R225W Mutation in Human AMPKγ3 Causing Increased Glycogen and Decreased Triglyceride in Skeletal Muscle

BACKGROUND: AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme that is evolutionarily conserved from yeast to mammals and functions to maintain cellular and whole body energy homeostasis. Studies in experimental animals demonstrate that activation of AMPK in skeletal muscle protects against insulin resistance, type 2 diabetes and obesity. The regulatory gamma(3) subunit of AMPK is expressed exclusively in skeletal muscle; however, its importance in controlling overall AMPK activity is unknown. While evidence is emerging that gamma subunit mutations interfere specifically with AMP activation, there remains some controversy regarding the impact of gamma subunit mutations. Here we report the first gain-of-function mutation in the muscle-specific regulatory gamma(3) subunit in humans. METHODS AND FINDINGS: We sequenced the exons and splice junctions of the AMPK gamma(3) gene (PRKAG3) in 761 obese and 759 lean individuals, identifying 87 sequence variants including a novel R225W mutation in subjects from two unrelated families. The gamma(3) R225W mutation is homologous in location to the gamma(2)R302Q mutation in patients with Wolf-Parkinson-White syndrome and to the gamma(3)R225Q mutation originally linked to an increase in muscle glycogen content in purebred Hampshire Rendement Napole (RN-) pigs. We demonstrate in differentiated muscle satellite cells obtained from the vastus lateralis of R225W carriers that the mutation is associated with an approximate doubling of both basal and AMP-activated AMPK activities. Moreover, subjects bearing the R225W mutation exhibit a approximately 90% increase of skeletal muscle glycogen content and a approximately 30% decrease in intramuscular triglyceride (IMTG). CONCLUSIONS: We have identified for the first time a mutation in the skeletal muscle-specific regulatory gamma(3) subunit of AMPK in humans. The gamma(3)R225W mutation has significant functional effects as demonstrated by increases in basal and AMP-activated AMPK activities, increased muscle glycogen and decreased IMTG. Overall, these findings are consistent with an important regulatory role for AMPK gamma(3) in human muscle energy metabolism