Hematological Parameters for COVID-19 Cases

Over the past few decades, the Corona virus has emerged as a significant global health concern, leading to severe respiratory illness and long-term health complications. Thoroughly analyzing hematology indices helps medical professionals develop personalized treatment plans and deliver specialized care to patients in critical conditions. Identifying potentially life-threatening complications, such as disseminated intravascular coagulation, early on and intervening effectively can greatly enhance the patient\u27s prognosis. According to a source, there is a reference to a specific point or piece of information. Therefore, the objective of this study was to assess the HT-P and their relationship with the clinical profile, enabling clinicians to determine the severity and prognosis of patients with COVID-19. Therefore, our study has led us to the conclusion that closely monitoring these levels is beneficial for effective patient management. By understanding the relationships between these parameters and clinical profiles, healthcare professionals are able to evaluate the severity and prognosis of individuals with COVID-19 illnesses. DOI: https://doi.org/10.52710/seejph.49

Uterine inversion: a shocking aftermath of mismanaged third stage of labour

Background: To study the incidence, causes, clinical presentations, management and maternal morbidity and mortality associated with acute puerperal inversion of uterus.Methods: This retrospective study was conducted in Department of Obstetrics and Gynecology, G.S.V.M. Medical College, Kanpur, from March 2008 to March 2013. All the women who developed acute puerperal inversion of uterus either in or outside our hospital were included in the study.Results: Majority of women presenting with inversion belonged to age group 20-35 years, were multigravida (68.2%), came from rural set-up (81.4%) and were unbooked (81.8%). Majority of deliveries complicated by inversion took place at home (50%), delivered by dais (46%). Mismanaged 3rd stage of labour proved to be an important factor (36.4%) leading to inversion uterus followed by atonicity of uterus. 90% of women suffered due to delay in management at PHC and CHC, 68%women succumbed to delay in diagnosis at the place of delivery by untrained staff, whereas 45% women suffered due to delay in transport facility. 13.63% women suffered from sepsis in post-operative period, 22.72% from renal failure whereas 18.18% died.Conclusion: Proper education and training regarding active management of third stage of labour, diagnosis and management of uterine inversion should be imparted to traditional birth attendants, so that this potentially life-threatening obstetric emergency could be averted

Supporting Collaborative Health Tracking in the Hospital: Patients' Perspectives

The hospital setting creates a high-stakes environment where patients' lives depend on accurate tracking of health data. Despite recent work emphasizing the importance of patients' engagement in their own health care, less is known about how patients track their health and care in the hospital. Through interviews and design probes, we investigated hospitalized patients' tracking activity and analyzed our results using the stage-based personal informatics model. We used this model to understand how to support the tracking needs of hospitalized patients at each stage. In this paper, we discuss hospitalized patients' needs for collaboratively tracking their health with their care team. We suggest future extensions of the stage-based model to accommodate collaborative tracking situations, such as hospitals, where data is collected, analyzed, and acted on by multiple people. Our findings uncover new directions for HCI research and highlight ways to support patients in tracking their care and improving patient safety

Genome-wide identification and expression analysis of the NHX gene family under salt stress in wheat (Triticum aestivum L)

Salt stress affects plant growth and development, resulting in the loss of crop yield across the world, and sodium-proton antiporters (NHXs) are one of the genes known to promote salt tolerance in transgenic plants. In this study, we conducted a comprehensive genome-wide analysis and expression profile of NHX genes in wheat under salinity stress. We identified 30 TaNHX genes in wheat based on the Na+/H+ exchanger domain, with all genes containing an amiloride motif except one, a known for inhibiting Na+ ions in plants. Phylogenetic analysis classified these genes into three classes with subfamilies: 12 were localized in vacuoles, while 18 were in the endoplasmic reticulum and plasma membrane. Promoter analysis revealed stress-related cis-acting elements, indicating their potential role in abiotic stress tolerance. The non-synonymous (Ka)/synonymous (Ks) ratios highlighted that the majority of TaNHX genes experienced robust purifying selection throughout their evolutionary history. Transcriptomis data analysis and qRT-PCR demonstrated distinct expression patterns for TaNHX genes across various tissues when subjected to salt stress. Additionally, we predicted 20 different miRNA candidates targeting the identified TaNHX genes. Protein-protein interaction prediction revealed NHX6’s involvement in the SOS1 pathway, while NHX1 gene exhibit proton antiporter activity. Molecular dynamics (MD) simulations were also conducted to examine the interactions of TaNHX1, TaNHX2, and TaNHX3. These results represent a significant advancement in our understanding of the molecular mechanisms governing Na+ transporters. This may also offer promising avenues for future studies aimed at unraveling the intricate details of their biological roles and applications

The Right To Information Act, 2005 And The Approach Of The Judiciary

On international right to know day an organization name as center for law & democracy issued a ranking for Right to information Act across the world and Indian Right to information Act got 4th position in the list. The very first RTI legislation was passed in Sweden which known as freedom of information Act 1776. In 1948 UN general Assembly passed a resolution and declares that right to information is fundamental human right. At present almost 111 countries have their own Right to information Ac

Prevalence Of Tuberculin Skin Test Positivity In Patients Of Early Rheumatoid Arthritis- Study from a tertiary care centre in North India.

Objective: To assess the prevalence of tuberculin skin test (TST) positivity in early rheumatoid arthritis patients (< 6months disease duration) using Tuberculin sensitivity testing in a TB endemic country. Method: Included in this cross-sectional study were 200 patients of early rheumatoid arthritis divided into three groups- treatment naïve, patients on methotrexate only and methotrexate plus low dose corticosteroids. Results: 200 early RA patients and 60 healthy controls were included in this study. The median age of the study population was 43 years (IQR 33-51) with a mean disease duration of 3.4 ± 2.1 months. 54 patients (27%) with early RA and 22 healthy control (36.7%) had TST positive (p=0.1). Conclusion: Tuberculin positivity is low among patients with early RA as compared to the general population. The use of low dose steroids or methotrexate doesn’t affect the tuberculin anergy

Stroke genetics informs drug discovery and risk prediction across ancestries

Previous genome-wide association studies (GWASs) of stroke — the second leading cause of death worldwide — were conducted predominantly in populations of European ancestry1,2. Here, in cross-ancestry GWAS meta-analyses of 110,182 patients who have had a stroke (five ancestries, 33% non-European) and 1,503,898 control individuals, we identify association signals for stroke and its subtypes at 89 (61 new) independent loci: 60 in primary inverse-variance-weighted analyses and 29 in secondary meta-regression and multitrait analyses. On the basis of internal cross-ancestry validation and an independent follow-up in 89,084 additional cases of stroke (30% non-European) and 1,013,843 control individuals, 87% of the primary stroke risk loci and 60% of the secondary stroke risk loci were replicated (P < 0.05). Effect sizes were highly correlated across ancestries. Cross-ancestry fine-mapping, in silico mutagenesis analysis3, and transcriptome-wide and proteome-wide association analyses revealed putative causal genes (such as SH3PXD2A and FURIN) and variants (such as at GRK5 and NOS3). Using a three-pronged approach4, we provide genetic evidence for putative drug effects, highlighting F11, KLKB1, PROC, GP1BA, LAMC2 and VCAM1 as possible targets, with drugs already under investigation for stroke for F11 and PROC. A polygenic score integrating cross-ancestry and ancestry-specific stroke GWASs with vascular-risk factor GWASs (integrative polygenic scores) strongly predicted ischaemic stroke in populations of European, East Asian and African ancestry5. Stroke genetic risk scores were predictive of ischaemic stroke independent of clinical risk factors in 52,600 clinical-trial participants with cardiometabolic disease. Our results provide insights to inform biology, reveal potential drug targets and derive genetic risk prediction tools across ancestries

Urolithin-A mitigates synaptic dysfunctions associated with epilepsy through VDAC1 inhibition: A novel therapeutic approach

Urolithin-A mitigates synaptic dysfunctions associated with epilepsy through VDAC1 inhibition: A novel therapeutic approach Epilepsies are a group of neurological disorders with a prevalence of 70 million people worldwide and are characterized by spontaneous, unprovoked, and variably synchronized seizures. Despite being presented with distinct aetiologies, epileptic seizures occur due to imbalances in excitation/inhibition (E/I) activity that disrupts neuronal ensemble activity. A large group of anti-epileptic medications (AEMs) available in the market constitutes a symptomatic approach by targeting the E/I imbalance involved in seizure generation and propagation, mediated by ion channels and neurotransmitter receptors. However, these drugs produce undesirable secondary effects and are ineffective in treating 30% of patients. Therefore, the development of new adjuvant drugs is required that act on other novel components of underlying pathogenesis: oxidation, mitochondrial dysfunctions, inflammation, apoptosis, and activity-dependent synaptic modifications. Such novel approaches might provide a homeostatic boost to the neuronal network and restore physiological imbalances. Recently, interest in compounds of natural origin for anti-epileptic potential has renewed, owing to less toxicity, safer profiles, and the probability of finding novel targets. Thus, identifying safer and tolerable natural products targeting the novel downstream mediators represents a promising treatment approach against epilepsy. While the pathogenesis of epilepsy is highly complex, with multiple stage-specific levels of anatomic, transcriptomic, and circuitry changes, aberrations in the synaptic transmission represent an essential common hallmark of initiation, epileptogenesis, and chronic illness phases of epilepsy. Studies on the protein-protein interactions have identified Punica granatum (Pomegranate) as one of the several natural products targeting synaptic proteins with anti-epileptic treatment potential. Additionally, ellagic acid (the primary polyphenolic precursor of UA found in Pomegranate) has been shown to exert anti-epileptic effects in epileptic models of mice. However, parental extracts undergo reduced bioavailability in the plasma; therefore, several studies have shown that the beneficial effects of the precursors are primarily mediated by gut microbial catabolite product, Urolithin-A (UA). UA supplementation has been shown to benefit central nervous system (CNS) disorders such as Parkinson’s and Alzheimer’s disease, attenuating oxidative stress in neuronal cells; however, its effects on epilepsy remain elusive. In this study, we show that the UA exerts anti-epileptic effects with disease-modifying potential resulting in fewer seizure-susceptible neuronal activity in different experimental models of epilepsy with diverse aetiologies. Anti-epileptic results were scored based on behavioral paradigms and studies of synaptic transmission at both structural and functional levels in the sub-acute picrotoxin-induced chemoconvulsive and chronic genetic models with a gain of function in sodium channel bang senseless (bss1). UA administration had no effects on the epileptiform firing induced in acute brain slices of mice, indicating that the anti-epileptic effects of UA are, indeed, through target mediators of the underlying pathogenesis of seizures; therefore, slow in action. Furthermore, the results were validated in the Pentylenetetrazol (PTZ)-induced chronic kindling mouse model. Extracellular and whole-cell patch clamp electrophysiology studies have shown that the effects mediated by UA were restricted to the post-synaptic level without affecting pre-synaptic functions. Using a combination of affinity purification with synthesized UA-attached beads and molecular-docking studies, we identified a complex of voltage-dependent anion channel-1 (VDAC-1) and β-Tubulin as the direct interactor of UA in the mouse brain. Transcriptomics studies in Drosophila and mice revealed disruption in the regulation of several downstream targets hinting at VDAC-1 inhibition. Finally, VDAC-1 knockdown in the sub-acute epileptic model of Drosophila mimics the effects of UA. Thus, our study provides an exciting and novel platform for developing effective and potent anti-epileptic therapy of Urolithin-A mediated modulation of VDAC1

Effect of Different Concentration of Major and Minor Nutrients on the Growth and Yield of Strawberry (Fragaria x ananassa L.) Grown under Aeroponic System

Strawberry is a perennial fruit crop of high importance in the market. It is highly recommended both as table and processed fruit due its nutrient content. The growth period of strawberry is short and hence doesn’t require much time for its growth and development. Aeroponic on the other hand seems to provide a better hand in cultivation of annual species in controlled conditions in a meticulous way.&nbsp; A field experiment was conducted at Horticulture Research Farm, Department of Horticulture, Naini Agriculture Institute, Sam Higginbottom university of agriculture, technology and science, Prayagraj, U.P during the year 2022-2023. The experiment comprised of eight different treatments with one control T1(3g NPK + 16g micronutrient), T2(6g NPK+14g micronutrient) T3(9g NPK+12g micronutrient), T4(12g NPK+10g micronutrient), T5(15g NPK+8g micronutrient) T6(18g NPK+6g micronutrient), T7(21g NPK+4g micronutrient), T8(24g NPK+2g micronutrient), T0 control (water). Based on the outcome of the current experiment, it can be concluded that treatment T6- (18gNPK+6gmicronutrient) produced the greatest results. It was determined to have the greatest growth traits with highest - Plant height(19.74cm), Number of leaves per plant (25.90), Plant spread(26.43cm) and Root length(28.35cm). It was also found best in terms of yield and yield attributing characters i.e., Total no flowers per plant (31.73), Fruit set (68.2 %) No of fruits per plant (21.58), Fruit weight (39.77g) Average yield (0.86kg)

A study of MHD fluid with second order slip and thermal flow over a nonlinear stretching sheet

An electrically conducted viscous incompressible nanofluid flow caused by the nonlinear stretching surface with stagnation flow has been investigated numerically. The effect of Brownian motion and thermophoresis on the nanofluid is also incorporated. The governing partial differential equations with nonlinear second order boundary conditions are solved by the fourth order Runge-Kutta technique using MATLAB programming. The effect of the radiation parameter (Rd), stretching parameter (n), Brownian motion parameter (Nb), thermophoresis parameter (Nt) on temperature, velocity and mass transfer are shown graphically. The influence of some of these parameters on the local Nusselt number (−′(0)) and local Sherwood number (−′(0)) are shown by the graphs