959 research outputs found
Automatic trinocular 3D reconstruction of coronary artery centerlines from rotational X-ray angiography
Dilepton production in proton-proton collisions at BEVALAC energies
The dilepton production in elementary reactions at
BEVALAC energies GeV is investigated. The calculations
include direct decays of the vector mesons , , and , Dalitz decays of the -, -, -, -, and -mesons, and of the baryon resonances
. The subthreshold vector meson production cross sections in
collisions are treated in a way sufficient to avoid double counting with the
inclusive vector meson production. The vector meson dominance model for the
transition form factors of the resonance Dalitz decays is
used in an extended form to ensure correct asymptotics which are in agreement
with the quark counting rules. Such a modification gives an unified and
consistent description of both radiative decays and meson decays.
The effect of multiple pion production on the experimental efficiency for the
detection of the dilepton pairs is studied. We find the dilepton yield in
reasonable agreement with the experimental data for the set of intermediate
energies whereas at the highest energy GeV the number of
dilepton pairs is likely to be overestimated experimentally in the mass range
MeV.Comment: 25 pages (IOP style), 5 figures, revised manuscript accepted for
publication in JP
Novel role for the innate immune receptor toll-like receptor 4 (TLR4) in the regulation of the wnt signaling pathway and photoreceptor apoptosis
Recent evidence has implicated innate immunity in regulating neuronal survival in the brain during stroke and other neurodegenerations. Photoreceptors are specialized light-detecting neurons in the retina that are essential for vision. In this study, we investigated the role of the innate immunity receptor TLR4 in photoreceptors. TLR4 activation by lipopolysaccharide (LPS) significantly reduced the survival of cultured mouse photoreceptors exposed to oxidative stress. With respect to mechanism, TLR4 suppressed Wnt signaling, decreased phosphorylation and activation of the Wnt receptor LRP6, and blocked the protective effect of the Wnt3a ligand. Paradoxically, TLR4 activation prior to oxidative injury protected photoreceptors, in a phenomenon known as preconditioning. Expression of TNFΞ± and its receptors TNFR1 and TNFR2 decreased during preconditioning, and preconditioning was mimicked by TNFΞ± antagonists, but was independent of Wnt signaling. Therefore, TLR4 is a novel regulator of photoreceptor survival that acts through the Wnt and TNFΞ± pathways. Β© 2012 Yi et al
Plx1 is required for chromosomal DNA replication under stressful conditions
Polo-like kinase (Plk)1 is required for mitosis progression. However, although Plk1 is expressed throughout the cell cycle, its function during S-phase is unknown. Using Xenopus laevis egg extracts, we demonstrate that Plx1, the Xenopus orthologue of Plk1, is required for DNA replication in the presence of stalled replication forks induced by aphidicolin, etoposide or reduced levels of DNA-bound Mcm complexes. Plx1 binds to chromatin and suppresses the ATM/ATR-dependent intra-S-phase checkpoint that inhibits origin firing. This allows Cdc45 loading and derepression of DNA replication initiation. Checkpoint activation increases Plx1 binding to the Mcm complex through its Polo box domain. Plx1 recruitment to chromatin is independent of checkpoint mediators Tipin and Claspin. Instead, ATR-dependent phosphorylation of serine 92 of Mcm2 is required for the recruitment of Plx1 to chromatin and for the recovery of DNA replication under stress. Depletion of Plx1 leads to accumulation of chromosomal breakage that is prevented by the addition of recombinant Plx1. These data suggest that Plx1 promotes genome stability by regulating DNA replication under stressful conditions
Automatic trinocular 3D reconstruction of coronary artery centerlines from rotational X-ray angiography
International audienceWe present a method for fully automatic 3D reconstruction of coronary artery centerlines using three X-ray angiogram projections from a single rotating monoplane acquisition. The reconstruction method consists of three steps: (1) filtering and segmenting the images using a multiscale analysis, (2) matching points in two of the segmented images using the information from the third image, and (3) reconstructing in 3D the matched points. This method needs good calibration of the system geometry and requires breatheld acquisitions. The final algorithm is formulated as an energy minimization problem that we solve using dynamic programming optimization. This method provides a fast and automatic way to compute 3D models of vessels centerlines. It has been applied to both phantoms, for validation purposes, and patient data sets
Orientational ordering of commensurate Fe(CO)5 monolayers on graphite
URL:http://link.aps.org/doi/10.1103/PhysRevB.27.5864
DOI:10.1103/PhysRevB.27.5864Elastic neutron diffraction and MΓΆssbauer spectroscopy have been used to study the structure, orientational-disordering (OD) transition, and melting of an Fe(CO)5 submonolayer adsorbed on Grafoil. The OD transition occurs between 150 and 167 K from a two-sublattice (β7Γβ21) structure to a nearly (β7Γβ7) phase in which the molecules are believed to rotate about the surface normal. MΓΆssbauer spectra exhibit a more abrupt variation with temperature near melting at 170 K than through the OD transition.This work was supported by NSF under Grants No. DMR-7905958 and No. INT-8012228, Israel-U.S. Binational Science Foundation Grant No. 2687, and the Danish Research Counci
Tissue Microenvironments Define and Get Reinforced by Macrophage Phenotypes in Homeostasis or during Inflammation, Repair and Fibrosis
Current macrophage phenotype classifications are based on distinct in vitro culture conditions that do not adequately mirror complex tissue environments. In vivo monocyte progenitors populate all tissues for immune surveillance which supports the maintenance of homeostasis as well as regaining homeostasis after injury. Here we propose to classify macrophage phenotypes according to prototypical tissue environments, e.g. as they occur during homeostasis as well as during the different phases of (dermal) wound healing. In tissue necrosis and/or infection, damage- and/or pathogen-associated molecular patterns induce proinflammatory macrophages by Toll-like receptors or inflammasomes. Such classically activated macrophages contribute to further tissue inflammation and damage. Apoptotic cells and antiinflammatory cytokines dominate in postinflammatory tissues which induce macrophages to produce more antiinflammatory mediators. Similarly, tumor-associated macrophages also confer immunosuppression in tumor stroma. Insufficient parenchymal healing despite abundant growth factors pushes macrophages to gain a profibrotic phenotype and promote fibrocyte recruitment which both enforce tissue scarring. Ischemic scars are largely devoid of cytokines and growth factors so that fibrolytic macrophages that predominantly secrete proteases digest the excess extracellular matrix. Together, macrophages stabilize their surrounding tissue microenvironments by adapting different phenotypes as feed-forward mechanisms to maintain tissue homeostasis or regain it following injury. Furthermore, macrophage heterogeneity in healthy or injured tissues mirrors spatial and temporal differences in microenvironments during the various stages of tissue injury and repair. Copyright (C) 2012 S. Karger AG, Base
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