Development of a marker panel for genotyping of domestic soybean cultivars for genes controlling the duration of vegetation and response to photoperiod

Soybean, Glycine max L., is one of the most important agricultural crops grown in a wide range of latitude. In this regard, in soybean breeding, it is necessary to pay attention to the set of genes that control the transition to the flowering stage, which will make it possible to adapt genotypes to local growing conditions as accurately as possible. The possibilities of soybean breeding for this trait have now significantly expanded due to identification of the main genes (E1–E4, GmFT2a, GmFT5a) that control the processes of flowering and maturation in soybean, depending on the day length. The aim of this work was to develop a panel of markers for these genes, which could be used for a rapid and efficient genotyping of domestic soybean cultivars and selection of plant material based on sensitivity to photoperiod and the duration of vegetation. Combinations of 10 primers, both previously developed and our own, were tested to identify different alleles of the E1–E4, GmFT2a, and GmFT5a genes using 10 soybean cultivars from different maturity groups. As a result, 5 combinations of dominant and recessive alleles for the E1–E4 genes were identified: (1) e1-nl(e1-as)/ e2-ns/e3-tr(e3-fs)/e4; (2) e1-as/e2-ns/e3-tr/E4; (3) e1-as/e2-ns/E3-Ha/e4; (4) E1/e2-ns/e3-tr/E4; (5) e1-nl/e2-ns/E3-Ha/E4. The studied cultivars contained the most common alleles of the GmFT2a and GmFT5a genes, with the exception of the ‘Cassidi’ cultivar having a rare dominant allele GmFT5a-H4. The degree of earliness of cultivars positively correlated with the number of recessive genes E1–E4, which is consistent with the data of foreign authors on different sets of cultivars from Japan and North China. Thus, the developed panel of markers ca

Meteoprotective properties of melaxen in old and middle aged patients with ischemic heart disease in combination with arterial hypertension

We studied 102 patients (mean age 60.1±3.3 years) with arterial hypertension (AH) II-III stage, grade 2-3 and ischemic heart disease (exertional angina functional class (FC) I-II, postinfarction cardiosclerosis). The control group of patients received traditional therapy (TT): beta -blockers, calcium channel blockers, ACE inhibitors, antiplatelet agents, diuretics and nitrate

First search for double-beta decay of 184Os and 192Os

A search for double-beta decay of osmium has been realized for the first time with the help of an ultra-low background HPGe gamma detector at the underground Gran Sasso National Laboratories of the INFN (Italy). After 2741 h of data taking with a 173 g ultra-pure osmium sample limits on double-beta processes in 184Os have been established at the level of T_{1/2} about 10^{14}-10^{17} yr. Possible resonant double-electron captures in 184Os were searched for with a sensitivity T_{1/2} about 10^{16} yr. A half-life limit T_{1/2} > 5.3 10^{19} yr was set for the double-beta decay of 192Os to the first excited level of 192Pt. The radiopurity of the osmium sample has been investigated and radionuclides 137Cs, 185Os and 207Bi were detected in the sample, while activities of 40K, 60Co, 226Ra and 232Th were limited at the mBq/kg level.Comment: 12 pages, 7 figures, 2 table

Search for 2\beta\ decays of 96Ru and 104Ru by ultra-low background HPGe gamma spectrometry at LNGS: final results

An experiment to search for double beta decay processes in 96Ru and 104Ru, which are accompanied by gamma rays, has been realized in the underground Gran Sasso National Laboratories of the I.N.F.N. (Italy). Ruthenium samples with masses of about (0.5-0.7) kg were measured with the help of ultra-low background high purity Ge gamma ray spectrometry. After 2162 h of data taking the samples were deeply purified to reduce the internal contamination of 40K. The last part of the data has been accumulated over 5479 h. New improved half life limits on 2\beta+/\epsilon \beta+/2\epsilon\ processes in 96Ru have been established on the level of 10^{20} yr, in particular for decays to the ground state of 96Mo: T1/2(2\nu 2\beta+) > 1.4 10^{20} yr, T1/2(2\nu \epsilon\beta+) > 8.0 10^{19} yr and T1/2(0\nu 2K) > 1.0 10^{21} yr (all limits are at 90% C.L.). The resonant neutrinoless double electron captures to the 2700.2 keV and 2712.7 keV excited states of 96Mo are restricted as: T1/2(0\nu KL) > 2.0 10^{20} yr and T1/2(0\nu 2L) > 3.6 10^{20} yr, respectively. Various two neutrino and neutrinoless 2\beta\ half lives of 96Ru have been estimated in the framework of the QRPA approach. In addition, the T1/2 limit for 0\nu 2\beta- transitions of 104Ru to the first excited state of 104Pd has been set as > 6.5 10^{20} yr.Comment: 14 pages, 5 figures, 2 tables; version accepted for publication on Phys. Rev.

Justification of Preventive Antiparasitic Measures on Recreational Waters

Aim: the visibility of the results of scientific rationale and development of antiparastic events on a specific model of recreational water bodies of Kharkiv region with emphasis the necessity of accounting the problem of condition monitoring of sand on the beaches. Materials and methods: the state of pollution of the sand was estimated according to the following criteria: salts of heavy metals, organic compounds, pathogens (E. coli, Enterococcus, Shigella, and Salmonella), the presence of worms dangerous to humans. Laboratory study of sand were conducted before and after the swimming season. Results. According to the results of the research conducted in the period 2010-2014 it was found that out of 2333 samples of sand (soil) made in the locations of beaches on parasitological research, 173 - discovered threat to human health helminths (7,41%)

Planar and 3D fibrous polyaniline-based materials for memristive elements

We discuss the effect of structure formation of Langmuir polyaniline layers on the performance of memristive thin-film elements as well as the morphology and conductivity of electrospinned PANI–PEO nonwovens

Search for Rare Nuclear Decays with HPGe Detectors at the STELLA Facility of the LNGS

Results on the search for rare nuclear decays with the ultra low background facility STELLA at the LNGS using gamma ray spectrometry are presented. In particular, the best T1/2 limits were obtained for double beta processes in 96Ru and 104Ru. Several isotopes, which potentially decay through different double beta decay channels, including also possible resonant double electron captures, were investigated for the first time (156Dy, 158Dy, 184Os, 192Os, 190Pt, 198Pt). Search for resonant absorption of solar 7Li axions in a LiF crystal gave the best limit for the mass of 7Li axions (< 8.6 keV). Rare alpha decay of 190Pt to the first excited level of 186Os (Eexc = 137.2 keV) was observed for the first time.Comment: 4 pages, contribution to the MEDEX'13 Conference, corrected list of author