Draft Genome Sequence of Antarctic Methanogen Enriched from Dry Valley Permafrost

A genomic reconstruction belonging to the genus Methanosarcina was assembled from metagenomic data from a methane-producing enrichment of Antarctic permafrost. This is the first methanogen genome reported from permafrost of the Dry Valleys and can help shed light on future climate-affected methane dynamics

Ruthenibacterium lactatiformans gen. nov., sp.nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces

Two novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces of healthy human subjects. The strains, designated as 585-1T and 668, were characterized by mesophilic fermentative metabolism, production of d-lactic acid, succinic acid and acetic acid as end products of d-glucose fermentation, prevalence of C18 : 1 ω9, C18 : 1 ω9 aldehyde, C16 : 0 and C16 : 1 ω7c fatty acids, presence of glycine, glutamic acid, lysine, alanine and aspartic acid in the petidoglycan peptide moiety and lack of respiratory quinones. Whole genome sequencing revealed the DNA G+C content was 56.4–56.6 mol%. The complete 16S rRNA gene sequences of the two strains shared 91.7/91.6 % similarity with Anaerofilum pentosovorans FaeT, 91.3/91.2 % with Gemmiger formicilis ATCC 27749T and 88.9/88.8 % with Faecalibacterium prausnitzii ATCC 27768T. On the basis of chemotaxonomic and genomic properties it was concluded that the strains represent a novel species in a new genus within the family Ruminococcaceae , for which the name Ruthenibacterium lactatiformans gen. nov., sp. nov. is proposed. The type strain of Ruthenibacterium lactatiformans is 585-1T (=DSM 100348T=VKM B-2901T)

АССОЦИАЦИИ БИОМОЛЕКУЛ, СВЯЗАННЫХ С СЕКРЕТОРНОЙ АКТИВНОСТЬЮ ВИСЦЕРАЛЬНЫХ АДИПОЦИТОВ, С ЭЛЕКТРОФИЗИОЛОГИЧЕСКИМИ ПРИЗНАКАМИ МЕТАБОЛИЧЕСКИХ НАРУШЕНИЙ МИОКАРДА У ЛИЦ ДО 45 ЛЕТ

Highlights ECG signs of metabolic cardiomyopathies in men aged 25–44 years, regardless of age and waist circumference, are directly associated with the serum adipsin concentration and inversely associated with the concentration of glucagon-like peptide 1 in the blood.ECG signs of metabolic cardiomyopathies in women aged 25–44 years are directly associated with the serum c-peptide concentration and inversely associated with the concentration of glucose-dependent insulinotropic polypeptide 1 in the blood. AbstractAim. To study the relationship between electrophysiological signs of metabolic cardiomyopathy (MC) and biomolecules associated with the secretory activity of visceral adipocytes in persons aged 25–44 years residing in Novosibirsk.Methods. The study included 1 198 subjects. A single-stage survey of a random sample of the 25–44-year-old population of Novosibirsk (49,7% men, 50,3% women) was conducted. During the examination, among other things, a resting 12-lead ECGs was recorded, and later the ECGs were classified according to Minnesota Code categories. Five ECG signs of MC were analysed: 1) ST-segment displaced above baseline (ST-segment elevation); 2) ST-segment displaced below baseline (ST-segment depression); 3) T wave changes; 4) TV1>TV6 patterns and 5) ECG signs of left ventricular hypertrophy. Serum concentrations of biomolecules associated with the secretory activity of visceral adipocytes were determined by multiplex analysis.Results. The results of multivariate logistic regression analysis showed that the ECG signs of MC in men aged 25–44 years, regardless of age and waist circumference, are directly associated with the serum adipsin concentration (Exp B 1,039, 95% CI 1,002–1,077, p = 0.039) and inversely associated with the concentration of glucagon-like peptide 1 in the blood (Exp B 0.999, 95% CI 0.998–1,000, p = 0.042). ECG signs of MC in women aged 25–44 years are directly associated with the serum peptide concentration (Exp B 1,439, 95% CI 1,082–1,915, p = 0.012) and inversely associated with the concentration of glucose-dependent insulinotropic polypeptide in the blood (Exp B 0.986, 95% CI 0.978–0.995, p = 0.001).Conclusion. The results obtained reflect the significant influence of these biomolecules associated with the secretory activity of visceral adipocytes on the occurrence of ECG signs of MC in young people aged 25–44 years.Основные положенияЭКГ-признаки метаболических нарушений миокарда у мужчин 25–44 лет независимо от возраста и окружности талии прямо ассоциированы с концентрацией в крови адипсина и обратно – с концентрацией глюкагоноподобного пептида 1.ЭКГ-признаки метаболических нарушений миокарда у женщин 25–44 лет прямо ассоциированы с концентрацией в крови C-пептида и обратно – с концентрацией глюкозозависимого инсулинотропного полипептида 1. Цель. Изучить ассоциации электрофизиологических признаков метаболических нарушений миокарда (МНМ) с биомолекулами, связанными с секреторной активностью висцеральных адипоцитов, у лиц 25–44 лет, проживающих в Новосибирске.Материалы и методы. Проведено одномоментное обследование случайной выборки населения Новосибирска в возрасте 25–44 лет (1 198 человек, 49,7% мужчин и 50,3% женщин). При обследовании в том числе проведена запись электрокардиограммы (ЭКГ) в покое в 12 стандартных отведениях с последующим кодированием по Миннесотскому коду. Проанализированы пять ЭКГ-признаков МНМ: смещение сегмента ST выше изолинии, депрессия сегмента ST ниже изолинии, изменения зубца Т, синдром TV1>TV6 и ЭКГ-признаки гипертрофии левого желудочка. В крови обследованных методом мультиплексного анализа определены концентрации биомолекул, ассоциированных с секреторной активностью висцеральных адипоцитов.Результаты. Результаты многофакторного логистического регрессионного анализа показали, что ЭКГ-признаки МНМ у мужчин 25–44 лет независимо от возраста и окружности талии прямо ассоциированы с концентрацией в крови адипсина (Exp B 1,039, 95% доверительный интервал (ДИ) 1,002–1,077; p = 0,039) и обратно – с концентрацией в крови глюкагоноподобного пептида 1 (Exp B 0,999, 95% ДИ 0,998–1,000; p = 0,042). Наличие ЭКГ-признаков МНМ у женщин 25–44 лет прямо ассоциировано с концентрацией в крови С-пептида (Exp B 1,439, 95% ДИ 1,082–1,915; p = 0,012) и обратно – с концентрацией в крови глюкозозависимого инсулинотропного полипептида (Exp B 0,986, 95% ДИ 0,978–0,995; p = 0,001).Заключение. Полученные результаты отражают значимое влияние биомолекул, ассоциированных с секреторной активностью висцеральных адипоцитов, на возникновение у людей 25–44 лет ЭКГ-признаков МНМ

Combination of searches for heavy spin-1 resonances using 139 fb−1 of proton-proton collision data at s = 13 TeV with the ATLAS detector

A combination of searches for new heavy spin-1 resonances decaying into different pairings of W, Z, or Higgs bosons, as well as directly into leptons or quarks, is presented. The data sample used corresponds to 139 fb−1 of proton-proton collisions at = 13 TeV collected during 2015–2018 with the ATLAS detector at the CERN Large Hadron Collider. Analyses selecting quark pairs (qq, bb, , and tb) or third-generation leptons (τν and ττ) are included in this kind of combination for the first time. A simplified model predicting a spin-1 heavy vector-boson triplet is used. Cross-section limits are set at the 95% confidence level and are compared with predictions for the benchmark model. These limits are also expressed in terms of constraints on couplings of the heavy vector-boson triplet to quarks, leptons, and the Higgs boson. The complementarity of the various analyses increases the sensitivity to new physics, and the resulting constraints are stronger than those from any individual analysis considered. The data exclude a heavy vector-boson triplet with mass below 5.8 TeV in a weakly coupled scenario, below 4.4 TeV in a strongly coupled scenario, and up to 1.5 TeV in the case of production via vector-boson fusion

Combination of searches for heavy spin-1 resonances using 139 fb−1 of proton-proton collision data at √s = 13 TeV with the ATLAS detector

A combination of searches for new heavy spin-1 resonances decaying into different pairings of W, Z, or Higgs bosons, as well as directly into leptons or quarks, is presented. The data sample used corresponds to 139 fb−1 of proton-proton collisions at √s = 13 TeV collected during 2015–2018 with the ATLAS detector at the CERN Large Hadron Collider. Analyses selecting quark pairs (qq, bb, tt¯, and tb) or third-generation leptons (τν and ττ) are included in this kind of combination for the first time. A simplified model predicting a spin-1 heavy vector-boson triplet is used. Cross-section limits are set at the 95% confidence level and are compared with predictions for the benchmark model. These limits are also expressed in terms of constraints on couplings of the heavy vector-boson triplet to quarks, leptons, and the Higgs boson. The complementarity of the various analyses increases the sensitivity to new physics, and the resulting constraints are stronger than those from any individual analysis considered. The data exclude a heavy vector-boson triplet with mass below 5.8 TeV in a weakly coupled scenario, below 4.4 TeV in a strongly coupled scenario, and up to 1.5 TeV in the case of production via vector-boson fusion

Combination of searches for heavy spin-1 resonances using 139 fb−1 of proton-proton collision data at √s = 13 TeV with the ATLAS detector

A combination of searches for new heavy spin-1 resonances decaying into diferent pairings of W, Z, or Higgs bosons, as well as directly into leptons or quarks, is presented. The data sample used corresponds to 139 fb−1 of proton-proton collisions at √s = 13 TeV collected during 2015–2018 with the ATLAS detector at the CERN Large Hadron Collider. Analyses selecting quark pairs (qq, bb, tt¯, and tb) or third-generation leptons (τν and τ τ ) are included in this kind of combination for the frst time. A simplifed model predicting a spin-1 heavy vector-boson triplet is used. Cross-section limits are set at the 95% confdence level and are compared with predictions for the benchmark model. These limits are also expressed in terms of constraints on couplings of the heavy vector-boson triplet to quarks, leptons, and the Higgs boson. The complementarity of the various analyses increases the sensitivity to new physics, and the resulting constraints are stronger than those from any individual analysis considered. The data exclude a heavy vector-boson triplet with mass below 5.8 TeV in a weakly coupled scenario, below 4.4 TeV in a strongly coupled scenario, and up to 1.5 TeV in the case of production via vector-boson fusion