A cellulose-based bioassay for the colorimetric detection of pathogen DNA

Cellulose-paper-based colorimetric bioassays may be used at the point of sampling without sophisticated equipment. This study reports the development of a colorimetric bioassay based on cellulose that can detect pathogen DNA. The detection was based on covalently attached single-stranded DNA probes and visual analysis. A cellulose surface functionalized with tosyl groups was prepared by the N,N-dimethylacetamide-lithium chloride method. Tosylation of cellulose was confirmed by scanning electron microscopy, Fourier transform infrared spectroscopy and elemental analysis. Sulfhydryl-modified oligonucleotide probes complementary to a segment of the DNA sequence IS6110 of Mycobacterium tuberculosis were covalently immobilized on the tosylated cellulose. On hybridization of biotin-labelled DNA oligonucleotides with these probes, a colorimetric signal was obtained with streptavidin-conjugated horseradish peroxidase catalysing the oxidation of tetramethylbenzamidine by H2O2. The colour intensity was significantly reduced when the bioassay was subjected to DNA oligonucleotide of randomized base composition. Initial experiments have shown a sensitivity of 0.1 μM. A high probe immobilization efficiency (more than 90 %) was observed with a detection limit of 0.1 μM, corresponding to an absolute amount of 10 pmol. The detection of M. tuberculosis DNA was demonstrated using this technique coupled with PCR for biotinylation of the DNA. This work shows the potential use of tosylated cellulose as the basis for point-of-sampling bioassays.Peer reviewedFinal Accepted Versio

In silico structural evaluation of short cationic antimicrobial peptides

© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Cationic peptides with antimicrobial properties are ubiquitous in nature and have been studied for many years in an attempt to design novel antibiotics. However, very few molecules are used in the clinic so far, sometimes due to their complexity but, mostly, as a consequence of the unfavorable pharmacokinetic profile associated with peptides. The aim of this work is to investigate cationic peptides in order to identify common structural features which could be useful for the design of small peptides or peptido-mimetics with improved drug-like properties and activity against Gram negative bacteria. Two sets of cationic peptides (AMPs) with known antimicrobial activity have been investigated. The first reference set comprised molecules with experimentally-known conformations available in the protein databank (PDB), and the second one was composed of short peptides active against Gram negative bacteria but with no significant structural information available. The predicted structures of the peptides from the first set were in excellent agreement with those experimentally-observed, which allowed analysis of the structural features of the second group using computationally-derived conformations. The peptide conformations, either experimentally available or predicted, were clustered in an “all vs. all” fashion and the most populated clusters were then analyzed. It was confirmed that these peptides tend to assume an amphipathic conformation regardless of the environment. It was also observed that positively-charged amino acid residues can often be found next to aromatic residues. Finally, a protocol was evaluated for the investigation of the behavior of short cationic peptides in the presence of a membrane-like environment such as dodecylphosphocholine (DPC) micelles. The results presented herein introduce a promising approach to inform the design of novel short peptides with a potential antimicrobial activity.Peer reviewedFinal Published versio

Difficult and frustrating? Caring for those with chronic and complex conditions

Caring for people with chronic and complex health needs has become an ever-larger part of everyday nursing. The World Health Organization has identified common and preventable risk factors that are the primary cause of chronic illness worldwide—hypertension, tobacco use, hyperglycaemia, physical inactivity, and overweight or obesity. Nurses now need an expanded range of skills and expertise that will enable them to work effectively with people whose chronic and frequently complex health issues may be seen as difficult and challenging. This article invites you to think about your experiences of caring for people with chronic and complex conditions. Before you read any further, take a moment to think about your response to some initial questions. How would you describe your attempts to work with people with chronic and complex health needs? Do you find yourself struggling to understand when your patient doesn’t appear to be following professional health advice? What do you hear your colleagues say about chronic illness

Supporting the nationally mandated transition to competency-based nursing curricula in Egypt

Egypt has a substantive and rich history in nursing education with continuous evolution over many years. A government commitment to further enhance the capacity of the nursing workforce recently required all nursing curricula to be upgraded and presented in a competency-based format. In response to this directive, lead agencies in Egypt, including the Educational Development Fund and the Nursing Sector Group of the Supreme Council of Universities, have garnered internal and external nursing expertise to provide guidance in the curriculum reform process. The authors of this presentation are currently working in close partnership with several Egyptian nursing education providers supporting the transition to competency-based nursing education. Shifting from bio-medical style syllabi to competency-based curricula is a time intensive and multi-faceted process. Successful transition requires a base knowledge of the fundamentals of competency-based programme design and its associated pedagogies. An understanding of relevant definitions, curriculum development processes, mandatory curriculum inclusions, and assessment techniques are core requirements to the development processes. This presentation provides an oversight of these terminologies and processes. The presentation provides insights to policy makers and educators embarking on similar nursing curriculum reform and nursing workforce capacity development initiatives

A framework for cyclical nursing curriculum review across multi-site and multi-country campuses

Cyclical curriculum review and continuous course improvement is an unarguable measure of quality nursing education. Higher education and professional regulators across the Middle East and North Africa (MENA) and African (Afro) regions are increasingly mandating cyclical review within accreditation and programme approval standards. Many nursing education providers in the region operate multi-site and multi-country campuses. A key challenge is to ensure that programmes keep pace with international standards in nursing education, and, that inter-campus drift does not occur in programme content and quality. Cyclical review offers a process whereby programmes can be updated to current best practice and consistent cross-campus standards can be maintained. The review process described in this presentation arises from a Aga Khan University project designed to review the status of courses within its post registration RN to BScN programme. The University operates four direct delivery sites in Kenya, Tanzania, Uganda and Pakistan and provides mentoring support to programmes in Upper Egypt and Mozambique. The project sought to assess currency of course content and develop a nursing course review framework for use by any educational provider involved in multi-campus programme delivery. The approach engaged an expert review team including internal and globally sourced external experts. The team involved subject experts and curriculum development and pedagoloical experts. Key foci of the review framework are described including; expected student outcomes, learning objectives, content inclusions, assessment items, and, teaching/learning resources. The review framework is of practical use to other educators involved in the review of course modules across multi-campus, multi-state, multi-emirate and/or multi-country settings

Public health service options for affordable and accessible noncommunicable disease and related chronic disease prevention and management

Globally, nations are confronted with the challenge of providing affordable health services to populations with increasing levels of noncommunicable and chronic disease. Paradoxically, many nations can both celebrate increases in life expectancy and bemoan parallel increases in chronic disease prevalence. Simply put, despite living longer, not all of that time is spent in good health. Combined with factors such as rising levels of obesity and related noncommunicable disease, the demand for health services is requiring nations to consider new models of affordable health care. Given the level of disease burden, all staff, not just doctors, need to be part of the solution and encouraged to innovate and deliver better and more affordable health care, particularly preventative primary health care services. This paper draws attention to a range of exemplars to encourage and stimulate readers to think beyond traditional models of primary health service delivery. Examples include nurse-led, allied health-led, and student-led clinics; student-assisted services; and community empowerment models. These are reported for the interest of policy makers and health service managers involved in preventative and primary health service redesign initiatives

Public health service options for affordable and accessible noncommunicable disease and related chronic disease prevention and management

Globally, nations are confronted with the challenge of providing affordable health services to populations with increasing levels of noncommunicable and chronic disease. Paradoxically, many nations can both celebrate increases in life expectancy and bemoan parallel increases in chronic disease prevalence. Simply put, despite living longer, not all of that time is spent in good health. Combined with factors such as rising levels of obesity and related noncommunicable disease, the demand for health services is requiring nations to consider new models of affordable health care. Given the level of disease burden, all staff, not just doctors, need to be part of the solution and encouraged to innovate and deliver better and more affordable health care, particularly preventative primary health care services. This paper draws attention to a range of exemplars to encourage and stimulate readers to think beyond traditional models of primary health service delivery. Examples include nurse-led, allied health-led, and student-led clinics; student-assisted services; and community empowerment models. These are reported for the interest of policy makers and health service managers involved in preventative and primary health service redesign initiatives

Qualitative Research: A pathway to gain an understanding of UAE mothers’ experiences and perceptions of managing a child newly diagnosed with Type 1 diabetes

A childhood diagnosis of Type 1 diabetes is a life changing event for both the child and his/her family. The impact is experienced strongly by mothers who most frequently fulfil the primary caregiver role for children12 yrs old and younger. Studies which have sought to understand mothers’ experiences and perceptions of managing a child newly diagnosed with Type 1 diabetes are mostly questionnaire-based and few, if any, explore the issues within the uniqueness of a Middle-Eastern context. The qualitative research methods described in this discussion have been designed to gain a better understanding of UAE mothers’ experience in managing a child newly diagnosed with Type 1 diabetes. The intended research will provide the health service entity with the opportunity to review existing services and further enhance the service and nursing care provided to this growing clientele group

The role and value of nurses in care provision: Views and expectations of Emirati Nationals in the western region of Abu Dhabi, United Arab Emirates

Background: Reliable health user information is needed to effectively organize nursing services and deliver quality patientcentered care. Nurse leaders of Al Gharbia Health Services (AGHS) noted that although budget data and general workforce information were readily available, detailed information about patient experiences, views, and expectations was lacking. Objective: This study aimed to obtain preliminary data regarding health and nursing service expectations of Emirati nationals in the remote western region of Abu Dhabi, United Arab Emirates (UAE). Methods: This study used qualitative descriptive methodology. Semi-structured interviews were conducted with a sample of Emirati families who had recently accessed AGHS. Interviews focused on service user understanding and expectations of the role of nurses, nursing services, and the health service overall. Results: Emirati nationals’ views and expectations regarding nurses’ role and function in the healthcare team were highlighted. Participants’ expectations and the attributes they valued were distributed across two distinct areas: clinical competence and the “softer” skills of compassion, caring, and respect. Conclusions: These findings facilitate understanding of the expectations of Emirati health service users. Importantly, the results counter regional perceptions of nursing under-valued as a servant role across the UAE and broader Gulf Cooperation Council region. The findings may inform a range of activities including: a large-scale survey regarding the views and expectations of Emirati health service users; pre-service nursing education and nursing in service development; and the recruitment of Emirati nationals to the nursing profession. Further investigation regarding perceptions and valuing of nursing within the Emirati community is warranted

Case-Based Insights: Arab Muslim Mothers’ Experiences of Managing a Child Newly Diagnosed With Type 1 Diabetes Mellitus

Mothers frequently fulfill the role of primary caregiver for children diagnosed with type 1 diabetes mellitus (T1DM). A T1DM diagnosis has a significant impact on the child and the wider family unit. The objective is to develop understanding of mothers’ experiences caring for children diagnosed with T1DM in the cultural context of the Middle East to facilitate enhanced health service provision and support. This study used a qualitative design. Data were collected in individual semistructured interviews. Participants were mothers of Arabic descent and Muslim belief who had a child diagnosed with T1DM within the last 12 months. All mothers were registered at the health service where this research was conducted and resident in the United Arab Emirates at the time of this study. COREQ guidelines informed reporting of the research and findings. Participating mothers described initial reactions of shock and disbelief, followed by transition to near ordinary and near normal (85% normal) family functioning. Family, culture, and faith emerged as critical supports in the whirlwind daily challenge of balancing the multiple demands and competing needs of the newly diagnosed child and the broader family. This study is the first of its kind from countries comprising the Gulf Cooperation Council. The findings provide insight into the challenges and support needs of mothers caring for children newly diagnosed with T1DM in an Arab Muslim context. The findings also provide a basis for enhancing health service support and suggest themes to inform further research