Roselle (Hibiscus sabdariffa L.) seeds as unconventional nutritional source

The composition of roselle seed from oil, protein, ash, fiber, fatty acids and amino acids was determined and compared in three cultivars in order to use it as an unconventional nutritional source. Aswan cv. occupies the highest significant rank in protein (31.51), oil (23.70) and fiber (4.87%) contents. Aswan and Sewa cvs. had the highest significant unsaturated fatty acid composition, especially oleic and linoleic acids, with oleic acid having values of 36.22 and 33.34% and linoleic acid, 14.95 and 15.10% values. Protein of Aswan cv. had the highest significant values of seven essential amino acids and four non-essential amino acids, especially lysine and phenylalanine.Key words: Amino acids, fatty acids, nutrition, oil, protein, roselle, unconventional crops

Active Power Filter Design by a Novel Approach of Multi-Objective Optimization

This paper presents an innovative active power filter design method to simultaneously compensate the current harmonics and reactive power of a nonlinear load. The power filter integrates a passive power filter which is a RL low-pass filter placed in series with the load, and an active power filter which comprises an RL in series with an IGBT based voltage source converter. The filter is assumed to inject a current into the connection node of the load and grid to eliminate current harmonics and its reactive part. The voltage source converter is placed in a hysteresis feedback control loop to generate a harmonic current. The bandwidth and output amplitude of the hysteresis controller are optimized with the inductance of RL filters. Three objective functions are considered in the optimization problem, which include minimizing of current total harmonic distortion, maximizing of power factor, and minimizing of the IGBT bridge current. For solving the optimization problem, two well-known multi-objective evolutionary algorithms are applied, namely, non-dominated sorting genetic algorithm-II (NSGA-II) and Strength Pareto Evolutionary Algorithm 2 (SPEA2). Test results showed that the SPEA2 technique exhibited a better performance in comparison to NSGA-II relative to the objectives

Sustainability of Scientific Journals in the Developing World With Special Reference to Bangladesh

The study explores sustainability of scientific journal publication in Bangladesh. Forty-three journal editors were interviewed, and 66 current journals were physically examined for production quality, regularity of publication, and availability at concerned libraries. Findings revealed that 68% of the journals were published late, 30% had inconsistencies in typesetting, and 14% were indexed. Most journals were found either excellent or of good quality in terms of printing (85%), binding (77%), paper (92%), and graphic reproduction (76%). Most journals were not available in major libraries under study. Of the 43 editors, 28 (35%) reported a cost recovery of 1-45% from subscriptions, advertisements, and sales. About 74.4% of the editors did not consider their journals at risk. Although 86% of the editors were confident that their journals would be sustained in the long run, 37.3% could not give any convincing logic in support of their statement. Major problems include lack of skilled staff, finance, quality articles and institutional support, and lengthy peer review process. Only one journal editor was found to be a full-time editor having training in editing and publication. One-half (51%) of the editors reported have training in editing, while four had publication training. Most editors (79%) were interested in acquiring training in editing and publication. Institutional support and backup, enthusiasm and zeal of editors, unmet need for standard local journals, constant flow of funds and articles, and skilled manpower are instrumental for sustainability of science journals in Bangladesh

Identification of p53-target genes in human papillomavirus-associated head and neck cancer by integrative bioinformatics analysis

Introduction: Head and neck cancer (HNC) is a highly prevalent and heterogeneous malignancy. Although extensive efforts have been made to advance its treatment, the prognosis remained poor with increased mortality. Human papillomaviruses (HPV) have been associated with high risk in HNC. TP53, a tumor suppressor, is the most frequently altered gene in HNC, therefore, investigating its target genes for the identification of novel biomarkers or therapeutic targets in HPV-related HNC progression is highly recommended. Methods: Transcriptomic profiles from three independent gene expression omnibus (GEO) datasets, including 44 HPV+ and 70 HPV- HNC patients, were subjected to integrative statistical and Bioinformatics analyses. For the top-selected marker, further in-silico validation in TCGA and GTEx databases and experimental validation in 65 (51 HPV- and 14 HPV+) subjects with histologically confirmed head and neck squamous cell carcinoma (HNSCC) have been performed. Results: A total of 498 differentially expressed genes (DEGs) were identified including 291 up-regulated genes and 207 down-regulated genes in HPV+ compared to HPV- HNSCC patients. Functional annotations and gene set enrichment analysis (GSEA) showed that the up-regulated genes were significantly involved in p53-related pathways. The integrative analysis between the Hub-genes identified in the complex protein-protein network and the top frequent genes resulting from GSEA showed an intriguing correlation with five biomarkers which are EZH2, MDM2, PCNA, STAT5A and TYMS. Importantly, the MDM2 gene showed the highest gene expression difference between HPV+ and HPV- HNSCC (Average log2FC = 1.89). Further in-silico validation in a large HNSCC cohort from TCGA and GTEx databases confirmed the over-expression of MDM2 in HPV+ compared to HPV- HNSCC patients (p = 2.39E-05). IHC scoring showed that MDM2 protein expression was significantly higher in HPV+ compared to HPV- HNSCC patients (p = 0.031). Discussion: Our findings showed evidence that over-expression of MDM2, proto-oncogene, may affect the occurrence and proliferation of HPV-associated HNSCC by disturbing the p53-target genes and consequently the p53-related pathways

DKK3’s protective role in prostate cancer is partly due to the modulation of immune-related pathways

While it is considered one of the most common cancers and the leading cause of death in men worldwide, prognostic stratification and treatment modalities are still limited for patients with prostate cancer (PCa). Recently, the introduction of genomic profiling and the use of new techniques like next-generation sequencing (NGS) in many cancers provide novel tools for the discovery of new molecular targets that might improve our understanding of the genomic aberrations in PCa and the discovery of novel prognostic and therapeutic targets. In this study, we investigated the possible mechanisms through which Dickkopf-3 (DKK3) produces its possible protective role in PCa using NGS in both the DKK3 overexpression PCa cell line (PC3) model and our patient cohort consisting of nine PCa and five benign prostatic hyperplasia. Interestingly, our results have shown that DKK3 transfection-modulated genes are involved in the regulation of cell motility, senescence-associated secretory phenotype (SASP), and cytokine signaling in the immune system, as well as in the regulation of adaptive immune response. Further analysis of our NGS using our in vitro model revealed the presence of 36 differentially expressed genes (DEGs) between DKK3 transfected cells and PC3 empty vector. In addition, both CP and ACE2 genes were differentially expressed not only between the transfected and empty groups but also between the transfected and Mock cells. The top common DEGs between the DKK3 overexpression cell line and our patient cohort are the following: IL32, IRAK1, RIOK1, HIST1H2BB, SNORA31, AKR1B1, ACE2, and CP. The upregulated genes including IL32, HIST1H2BB, and SNORA31 showed tumor suppressor functions in various cancers including PCa. On the other hand, both IRAK1 and RIOK1 were downregulated and involved in tumor initiation, tumor progression, poor outcome, and radiotherapy resistance. Together, our results highlighted the possible role of the DKK3-related genes in protecting against PCa initiation and progression

PhyloToAST: Bioinformatics Tools for Species-Level Analysis and Visualization of Complex Microbial Datasets

The 16S rRNA gene is widely used for taxonomic profiling of microbial ecosystems; and recent advances in sequencing chemistry have allowed extremely large numbers of sequences to be generated from minimal amounts of biological samples. Analysis speed and resolution of data to species-level taxa are two important factors in large-scale explorations of complex microbiomes using 16S sequencing. We present here new software, Phylogenetic Tools for Analysis of Species-level Taxa (PhyloToAST), that completely integrates with the QIIME pipeline to improve analysis speed, reduce primer bias (requiring two sequencing primers), enhance species-level analysis, and add new visualization tools. The code is free and open source, and can be accessed at http://phylotoast.org

Emerging wireless communication technologies in Iraqi government: Exploring cloud, edge, and fog computing

This study aims to structure the implementation of a governmental cloud of things (CoT), edge computing (EC), and fog computing in Iraq in the context of sustainable wireless communication. A base of literature was built that included any challenges, opportunities, and best practices relevant to these innovative technologies to set up the background for this paper. A concept model was created that included core components (cognitive technologies and fog computing), key processes (resource analysis, infrastructure design), and stakeholders (governments, industry, community). A strategic methodology made up of stakeholder involvement, capacity building, and pilot projects was used in the project. Concerning IoT planned deployment and services provision, network infrastructure was put in place to support the devices and a higher level of security measures were recommended. Using scenario hypothesis, MATLAB simulator was employed to simulate data value distribution as well as received power distribution based on different institutions for 12 months. Monitoring and evaluation should be followed to measure performance indicators and effects on this process. Continuously improvement strategies were the highlight of the session which further stimulated innovations. Acquainted projects will be put in the function to extend the range of activities by including additional government agencies, regions, or sectors. Reporting of the collected data and funding will be done with stakeholders to share and pool knowledge

Disaster management in Bangladesh: developing an effective emergency supply chain network

YesThis study has addressed and identified the problems in managing the existing emergency supply chain of Bangladesh in all phases of operation in terms of the primary drivers of the supply chain. It has also attempted to conceptualize and suggest an effective emergency supply chain. In this context, a thorough field investigation in several districts was conducted among the employees of the organizations sharing common information with similar protocols and implications (interoperable). Information was collected from the employees of all the participating organizations involved in disaster management through a semi-structured questionnaire based survey. The respondents addressed and illustrated several interconnected reasons which are inhibiting proper forecasting, procurement, storage, identification of affected people, and distribution. The respondents pointed out that the mismatching of objectives in the different organizations resulted in non-interoperability among the participating organizations. These issues are related to the malfunctioning of management with multidimensional organizational conflicts. Reflecting those issues, an emergency supply chain for disaster management is proposed in this stud