Translation of Jiuzhaigou Tourism Websites From the Perspective of Eco-translatology

Taking Jiuzhaigou tourism website as an example and Eco-translatology as guidance theory, this paper analyzes the tourism text from language, cultural and communicative dimensions to obtain suggestions for tourism text translation. In translating tourism text, translator should simplify language and adjust sequence of text in language dimension, add supplementary information of culture elements in cultural dimension, and use more second person pronoun and interrogative sentences in communicative dimension. Thus, the translation can be more acceptable to target readers so as to achieve commercial purpose of tourism text

Spatio-Temporal Usage Patterns of Dockless Bike-Sharing Service Linking to a Metro Station: A Case Study in Shanghai, China

The dockless bike-sharing (DLBS) system serves as a link between metro stations and travelers\u27 destinations (or originations). This paper aims to uncover spatio-temporal usage patterns of dockless bike-sharing service linking to metro stations for supporting scientific planning and management of the dockless bike-sharing system. A powerful visualization tool was used to analyze the differences in usage patterns in workdays and weekends. The travel distance distributions of using dockless bike-sharing near metro stations were investigated to shed light on the service area of the dockless bike-sharing system. Agglomerative hierarchical clustering was applied to analyze differences in usage patterns of metro stations located in different areas. The results show that the usage patterns of dockless bike-sharing on weekends are different from those on workdays. The average travel distance using the dockless bike-sharing system at weekends is significantly larger than that of workdays. The travel distance distribution could be nicely fitted by the Frechet distribution of the Generalized Extreme Value (GEV) distribution family. The usage characteristics of shared bikes are correlated with land use and population density around metro stations. No matter in urban or suburban areas, there is a great demand for bike-sharing in densely populated areas with intensive land development, such as university towns in suburban areas. This study improves the understandings regarding the usage patterns of the DLBS system serving as a link between the final destinations (or originations) and metro stations. The results can be helpful to the operation and demand management of DLBS. \ua9 2020 by the authors

Study on the Cooling Capacity of Different Quenchant

AbstractHeat treatment is one method to improve mechanical properties of metal, but each heat treatment method has its advantages and disadvantages, therefore, different requirements regarding size, shape, and properties with respect to different heat treatment processes should be considered. The traditional liquid quenchant are clear water and quench oil. Gas quenching is a relatively new process with several important advantages, such as minimal environmental impact, clean products, and ability to control the cooling locally and temporally for best product properties. To meet the high cooling rates required for quenching, the cooling gas must flow at very high velocities, but still the cooling capacity of gas is weakness. In order to increase the cooling capacity of gas, the spray water is added during gas quenching. In this paper, the GCr15 steel is as the research object, the cooling capacity of clear water, quench oil, nitrogen and nitrogen-spray water are studied through comparison of temperature difference and cooling velocity of the specimen

Generation and Bioenergetic Profiles of Cybrids with East Asian mtDNA Haplogroups

Human mitochondrial DNA (mtDNA) variants and haplogroups may contribute to susceptibility to various diseases and pathological conditions, but the underlying mechanisms are not well understood. To address this issue, we established a cytoplasmic hybrid (cybrid) system to investigate the role of mtDNA haplogroups in human disease; specifically, we examined the effects of East Asian mtDNA genetic backgrounds on oxidative phosphorylation (OxPhos). We found that mtDNA single nucleotide polymorphisms such as m.489T>C, m.10398A>G, m.10400C>T, m.C16223T, and m.T16362C affected mitochondrial function at the level of mtDNA, mtRNA, or the OxPhos complex. Macrohaplogroup M exhibited higher respiratory activity than haplogroup N owing to its higher mtDNA content, mtRNA transcript levels, and complex III abundance. Additionally, haplogroup M had higher reactive oxygen species levels and NAD+/NADH ratios than haplogroup N, suggesting difference in mitonuclear interactions. Notably, subhaplogroups G2, B4, and F1 appeared to contribute significantly to the differences between haplogroups M and N. Thus, our cybrid-based system can provide insight into the mechanistic basis for the role of mtDNA haplogroups in human diseases and the effect of mtDNA variants on mitochondrial OxPhos function. In addition, studies of mitonuclear interaction using this system can reveal predisposition to certain diseases conferred by variations in mtDNA

Dietary Lysozyme Alters Sow’s Gut Microbiota, Serum Immunity and Milk Metabolite Profile

The aim of current study was to determine variations in sow’s gut microbiota, serum immunity, and milk metabolite profile mediated by lysozyme supplementation. Twenty-four pregnant sows were assigned to a control group without supplementation and two treatments with 0.5 kg/t and 1.0 kg/t lysozyme provided in formula feed for 21 days (n = 8 per treatment). Microbiota analysis and metagenomic predictions were based on 16s RNA high-throughput sequencing. Milk metabolome was assessed by untargeted liquid chromatography tandem mass spectrometry. Serum biochemical indicators and immunoglobulins were also determined. Gut microbial diversity of sows receiving 1.0 kg/t lysozyme treatment was significantly reduced after the trial. Spirochaetes, Euryarchaeota, and Actinobacteria significantly increased while Firmicutes showed a remarkable reduction in 1.0 kg/t group compared with control. Lysozyme addition rebuilt sow’s gut microbiota to beneficial composition identified by reduced richness of Escherichia coli and increased abundance of Lactobacillus amylovorus. Accordingly, microbial metabolic functions including pyrimidine metabolism, purine metabolism, and amino acid related enzymes were significantly up-regulated in 1.0 kg/t group. Microbial metabolic phenotypes like the richness of Gram-positive bacteria and oxidative stress tolerance were also significantly reduced by lysozyme treatment. Serum alanine transaminase (ALT) activity and IgA levels were significantly down-regulated in the 1.0 kg/t group compared with control, but IgM levels showed a significantly increase in 1.0 kg/t group. Milk metabolites such as L-glutamine, creatine, and L-arginine showed significantly dose-dependent changes after treatment. Overall, lysozyme supplementation could effectively improve the composition, metabolic functions, and phenotypes of sow’s gut microbiota and it also benefit sows with better serum immunity and milk composition. This research could provide theoretical support for further application of lysozyme in promoting animal gut health and prevent pathogenic infections in livestock production

Dynamic measurement of gas volume fraction in a CO2 pipeline through capacitive sensing and data driven modelling

Gas volume fraction (GVF) measurement of gas-liquid two-phase CO2 flow is essential in the deployment of carbon capture and storage (CCS) technology. This paper presents a new method to measure the GVF of two-phase CO2 flow using a 12-electrode capacitive sensor. Three data driven models, based on back-propagation neural network (BPNN), radial basis function neural network (RBFNN) and least-squares support vector machine (LS-SVM), respectively, are established using the capacitance data. In the data pre-processing stage, copula functions are applied to select feature variables and generate training datasets for the data driven models. Experiments were conducted on a CO2 gas-liquid two-phase flow rig under steady-state flow conditions with the mass flowrate of liquid CO2 ranging from 200 kg/h to 3100 kg/h and the GVF from 0% to 84%. Due to the flexible operations of the power generation utility with CCS capabilities, dynamic experiments with rapid changes in the GVF were also carried out on the test rig to evaluate the real-time performance of the data driven models. Measurement results under steady-state flow conditions demonstrate that the RBFNN yields relative errors within ±7% and outperforms the other two models. The results under dynamic flow conditions illustrate that the RBFNN can follow the rapid changes in the GVF with an error within ±16%

Generation of dual-gRNA library for combinatorial CRISPR screening of synthetic lethal gene pairs

Combinatorial CRISPR screening is useful for investigating synthetic lethality (SL) gene pairs. Here, we detail the steps for dual-gRNA library construction, with the introduction of two backbones, LentiGuide_DKO and LentiCRISPR_DKO. We describe steps fo

A New Allele of the SPIKE1 Locus Reveals Distinct Regulation of Trichome and Pavement Cell Development and Plant Growth

The single-celled trichomes of Arabidopsis thaliana have long served as an elegant model for elucidating the mechanisms of cell differentiation and morphogenesis due to their unique growth patterns. To identify new components in the genetic network that governs trichome development, we carried out exhaustive screens for additional Arabidopsis mutants with altered trichome morphology. Here, we report one mutant, aberrantly branched trichome1-1 (abt1-1), with a reduced trichome branching phenotype. After positional cloning, a point mutation in the SPIKE1 (SPK1) gene was identified in abt1-1. Further genetic complementation experiments confirmed that abt1-1 is a new allele of SPK1, so abt1-1 was renamed as spk1-7 according to the literatures. spk1-7 and two other spk1 mutant alleles, covering a spectrum of phenotypic severity, highlighted the distinct responses of developmental programs to different SPK1 mutations. Although null spk1 mutants are lethal and show defects in plant stature, trichome and epidermal pavement cell development, only trichome branching is affected in spk1-7. Surprisingly, we found that SPK1 is involved in the positioning of nuclei in the trichome cells. Lastly, through double mutant analysis, we found the coordinated regulation of trichome branching between SPK1 and two other trichome branching regulators, ANGUSTIFOLIA (AN) and ZWICHEL (ZWI). SPK1 might serve for the precise positioning of trichome nuclei, while AN and ZWI contribute to the formation of branch points through governing the cMTs dynamics. In summary, this study presented a fully viable new mutant allele of SPK1 and shed new light on the regulation of trichome branching and other developmental processes by SPK1