Application of technologies of atomic force microscope investigation for evaluation of structure and properties of blood cells’ surfaces

With application of techniques of atomic force microscope investigation, we studied properties and topography of blood cells’ surfaces in patients with acute lymphoblastic and myeloblastic leucosis. It was stated that development of acute forms of lymphoblastic and myeloblastic types of proliferation is accompanied with reduction of stiffness of cell surface and increase of surface potentia

Evaluation of the elastic properties and topography of leukocytes’ surface in patients with type 2 diabetes mellitus using atomic force microscope

The aim of study was to examine some morphometrical parameters (height, diameter) of the leukocytes (white blood cells - WBCs), their specific surface morphology (globular prominences and depression in WBCs) as well as their local elastic properties (Young’s modulus) in healthy persons and in patients with type 2 diabetes mellitus (T2DM) by means of the atomic force microscopy (AFM). Morphological and morphometrical parameters of human leukocytes were evaluated by AFM in tapped mod

Study of the properties of doxorubicin-resistant cells affected by acute leucosis

The stiffness of cell membrane was found to be one of the factors determining resistance of a cell in vitro to antibiotic doxorubicin action.The data obtained have important prognostic value in studying drug resistance of tumour blood cells and can be used as objective markers of efficiency of the antitumor therap

ОПРЕДЕЛЕНИЕ ПАССАЖИРОПОТОКОВ В РЕГИОНАЛЬНОЙ ТРАНСПОРТНОЙ СИСТЕМЕ НА ОСНОВЕ МОДИФИЦИРОВАННЫХ ГРАВИТАЦИОННЫХ МОДЕЛЕЙ

The paper considers a transportation system of  one poorly developed region of the Eastern Siberia where it is planned to make investments for improvement of the corresponding infrastructure. The efficient investment disbursement presupposes study of the existing demand for transport services in the field of passenger traffic.  Results of the observations can be used for development planning of a road network and a bus service system.Due to lack of direct data on the existing demand efforts have been made to obtain its model description while using modified gravity models that make it possible to estimate  volumes of population transportation which is carried out between residential areas by public transport buses and private vehicles. The given models have permitted to make analysis of more than 5000 populated locality pairs for 86 residential areas where the population constitutes more than 80 % and its passenger flow formation component in equal to 60%. Traffic flows between these settlement pairs have been estimated with the help of gravity models with various attraction functions and Arrowsmith method for provision of transportation balances. The most adequate results have been obtained while using an exponential attraction function for individual vehicles and a power attraction function  for passenger route auto transport. Such approach is consistent with the preference of the route auto transport in case of long-distance transport service. A tree-like structure of the existing traffic system has given the possibility to avoid consideration of  alternative routes in case of passenger transportation and directly calculate transport flows for certain road sections. Comparative analysis of the transport flows using the proposed methodology and the existing partial data of the executed observations reveals  satisfactory coincidence of empirical data for the main part of the traffic system. The obtained results demonstrate an efficiency of the described approach and the presented data can be used as a benchmarking tool for transport planning.Рассмотрена транспортная система одного из слабоосвоенных регионов Восточной Сибири, для которого планируются инвестиции в развитие соответствующей инфраструктуры. Эффективное использование инвестируемых средств предполагает изучение имеющегося спроса на транспортные услуги в области пассажирских перевозок, результаты которого могут быть использованы в планировании развития дорожной сети и системы автобусного сообщения.В связи с отсутствием прямых данных об имеющемся спросе предпринята попытка его модельного описания с помощью модифицированных гравитационных моделей для определения объемов перемещения населения с использованием маршрутных автобусов и индивидуальных автомобилей между населенными пунктами. Всего в модели рассмотрено более 5000 корреспондирующих пар населенных пунктов для 86 населенных пунктов, где проживало свыше 80 % всего населения региона, из которых выделена потокообразующая часть в обьеме 60 %. Перевозки в корреспондирующих парах определяли на основе гравитационных моделей с различными функциями притяжения и использованием метода Эрроусмита для удовлетворения транспортных балансов. Наиболее адекватные результаты получены с помощью экспоненциальной функции притяжения для индивидуальных автомобилей и степенной – для маршрутизируемого пассажирского автотранспорта, что вполне согласуется с предпочтением маршрутизируемого автотранспорта для дальних перевозок. Древовидная структура имеющейся дорожной сети позволила избежать рассмотрения альтернативных маршрутов при перемещении пассажиров и непосредственно рассчитать транспортные потоки на определенных участках. Сравнение рассчитанных на этой основе транспортных потоков с имеющимися частичными данными наблюдений демонстрирует удовлетворительное совпадение на основном массиве эмпирических данных. Полученные результаты показывают эффективность подобного подхода, а сами данные могут служить ориентиром для транспортного планирования.

Application of technologies of atomic force microscope investigation for evaluation of structure and properties of blood cells’ surfaces

With application of techniques of atomic force microscope investigation, we studied properties and topography of blood cells’ surfaces in patients with acute lymphoblastic and myeloblastic leucosis. It was stated that development of acute forms of lymphoblastic and myeloblastic types of proliferation is accompanied with reduction of stiffness of cell surface and increase of surface potentia

Evaluation of the elastic properties and topography of leukocytes’ surface in patients with type 2 diabetes mellitus using atomic force microscope

The aim of study was to examine some morphometrical parameters (height, diameter) of the leukocytes (white blood cells - WBCs), their specific surface morphology (globular prominences and depression in WBCs) as well as their local elastic properties (Young’s modulus) in healthy persons and in patients with type 2 diabetes mellitus (T2DM) by means of the atomic force microscopy (AFM). Morphological and morphometrical parameters of human leukocytes were evaluated by AFM in tapped mod

The changes of ultrastructure and mechanical properties of lymphocytes' plasmalemma under the influence of chemotherapy

Atomic force microscopy has established the changes of mechanical properties of lymphocytes' membranes from patients with acute and chronic lymphoblastic leukaemia. The stiffness of cell membrane was increased under influence chemotherapy along with the ultrastructural organisation of the lymphocyte's surface was different between patients with acute and chronic leukaemi