14 research outputs found
Umbelliprenin-coated Fe3O4 magnetite nanoparticles: Antiproliferation evaluation on human Fibrosarcoma cell line (HT-1080)
The potential applications of Fe3O4 magnetite nanoparticles (MNPs) in nanomedicine as drug delivery systems
are well known. In this study we prepared umbelliprenin-coated Fe3O4 MNPs and evaluated the
antiproliferative effect of combination in vitro. After synthesis of Fe3O4 MNPs, particles were characterized
by transmission electron microscopy, energy-dispersive spectroscopy, and X-ray diffraction spectroscopy
techniques. The natural candidate compound — umbelliprenin— was isolated and identified and
umbelliprenin-coated Fe3O4 MNPs were prepared, using precipitation method. The surface chemistry of
umbelliprenin-coated Fe3O4 MNPs as well as their thermal decomposition characteristics was examined using
Fourier transform infrared spectroscopy and Thermogravimetric Analyzer equipment, respectively. HT-1080
cells were cultured until the logarithmic phase of growth, and MTT assay was successfully carried out to
evaluate the possible cytotoxic effects of umbelliprenin-coated Fe3O4 MNPs in viable cells in vitro. The
results demonstrated that umbelliprenin has moderate antiproliferative effects with IC50 value of 50 μg/mL.
However, the combination of umbelliprenin and Fe3O4 MNPs showed the IC50 value of 9 μg/mL. In other
words, cell proliferation decreased to the remarkably-low proportion of 45% after treating cells with
umbelliprenin-coated Fe3O4 MNPs. This suggests that with the aid of nanoparticles as carriers, natural
products may have even broader range of medical applications in future