Amendment to the recommendation for a Regulation of the Council concluding on Agreement between the European Economic Community and Macao on trade in textile products. COM (76) 572 final, 4 November 1976

Background:Adolescents are the most frequent fast food consumers. This unhealthy behavior lead to overweight that is associated with diabetes mellitus, hypertension, and heart diseases. The present study aimed to determine factors associated with behavior of fast food consumption (FFC) of female high school students based on the Theory of Planned Behavior (TPB) in the city of Tonekanon, north of Iran.Methods:The present cross-sectional study was conducted on 197 female students studying at high school. The constructs of the theory of planned behavior including attitude, subjective norms, perceived behavioral control, as well as intention and behavior of fast food consumption were assessed using a pre-tested psychometric questionnaire. Spearman correlation coefficient and linear regression method were used to test the study hypotheses.Results:The constructs of attitude, subjective norms, and perceived behavioral control were accounted for 37.8% in variation of fast food consumption intention. The construct of subjective norms and perceived behavior control was significantly associated with intention of FFC. Intention of FFC was significantly associated with behavior (B coefficient=0.76, P-value=0.001) and predicted 63.8 % of the variance of fast food consumption behavior.Conclusion:The present study showed that the TPB is a powerful theory in predicting FFC behavior. Subjective norms followed by perceived behavioral control were the strongest predictors of FFC intention

Diversidade genética para padrões de gliadinas de raças locais de trigo duro no Noroeste do Iran e no Azerbaijão

The objective of this study was to identify gliadin band patterns and the extent of genetic diversity in durum wheat genotypes from Northwestern Iran and the Republic of Azerbaijan. Gliadins from 46 landraces and four cultivars were evaluated through acid PAGE analyses. Sixty-six polymorphic bands and 81 patterns were identified. Twenty-four different motility bands and 22 patterns were found in the ω gliadin region with 14 polymorph bands and 20 patterns for α and γ gliadins, and 14 bands and 19 different patterns for β gliadins. The combination of these patterns generated 38 and 39 combinations for Gli-1 and Gli-2 loci, respectively. The genetic diversity index (H) was higher for α gliadins (0.924), followed by ω and γ gliadins (0.899 and 0.878, respectively), and for β gliadin patterns (0.866). Extensive polymorphism (H = 0.875) was observed in four gliadin pattern regions, with higher genetic diversity in the Iranian landraces than in the Azerbaijani ones. Each genotype had special identifying patterns in the gliadin acid PAGE analysis, and cluster analysis based on Jaccard's similarity coefficients formed six groups. Gliadin has a simple, repeatable and economic analysis, and can be used in genetic studies.O objetivo deste trabalho foi identificar padrões de bandas de gliadinas e a extenção da variabilidade genética em genótipos de trigo duro do Noroeste do Iran e da República do Azerbaijão. Gliadinas de 46 raças locais e de quatro cultivares foram avaliadas por meio de análises "acid PAGE". Sessenta e seis bandas polimórficas e 81 padrões foram identificados. Foram encontradas 24 bandas de mobilidade e 22 padrões na região da gliadina ω, com 14 bandas polimorfas e 20 padrões para as gliadinas α e γ , e 14 bandas e 19 padrões diferentes para as gliadinas β. A combinação desses padrões gerou 38 e 39 combinações, para os locos Gli-1 e Gli-2, respectivamente. O índice de diversidade genética (H) foi maior para as gliadinas α (0,924), seguidas pelas gliadinas ω e γ (0,899 e 0,878, respectivamente), e pela gliadina β (0,866). Alto polimorfismo (H = 0,875) foi observado em quatro regiões dos padrões de gliadinas obtidos, com diversidade genética maior nas raças locais iranianas do que nas do Azerbaijão. Cada genótipo apresentou padrões de identificação especiais na análise "acid PAGE" de gliadinas, e a análise de agrupamento baseada no coeficiente de similaridade de Jaccard formou seis grupos. A gliadina é de análise simples, repetível e econômica, e pode ser usada em estudos genéticos

Synthesis of zero-valent iron nanoparticles via electrical wire explosion for efficient removal of heavy metals

In this paper, electrical wire explosion (EWE) was used as a simple, clean, in situ method to synthesise nano zero-valent iron (nZVI) particles in liquid mediums. The structural and physical properties of the synthesised nZVI particles were characterised by x-ray diffraction, Brunauer–Emmett–Teller analysis, field emission scanning electron microscopy and transmission electron microscopy. The synthesised spherical nanoparticles, at a specific size of 10–50 nm, possessed large surface areas of approximately 13.27 m/g, reinforcing the significant advantages of EWE, that is, less aggregation and in situ production of nanoparticles with enhanced removal efficacy, for groundwater treatment. After 1 h the adsorption capacity for Cr(VI), Ni(II), and Cd(II) (Q = C/C (mg/g)) at pH 4 was approximately 143.4 (89.0%), 137.5 (79.0%), and 132.6 mg/g (72.0%), respectively. The effect of temperature was also examined at initial metal ions concentration of 70 mg/L, nZVI dosage of 0.2 g/L and pH 4. The results showed 45°C was the optimum temperature for adsorption of Cr(VI), Ni(II), and Cd(II) ions, and over a 60-min period at this temperature adsorption capacity increased to 153.56 (95.3%), 158.91 (91.3%), and 163.90 mg/g (89.0%), respectively

<i>O</i>-prenylated 3-carboxycoumarins as a novel class of 15-LOX-1 inhibitors

<div><p>Allyloxy, Isopentenyloxy, geranyloxy and farnesyloxy derivatives of 3-carboxycoumarin, at position 5, 6, 7, and 8, were synthesized and their inhibitory potency against human 15-lipoxygenase-1 (human 15-LOX-1) were determined. Among the synthetic coumarins, <i>O</i>-allyl and <i>O</i>-isopentenyl derivatives demonstrated no considerable lipoxygenase inhibition while <i>O</i>-geranyl and <i>O</i>-farnesyl derivatives demonstrated potent inhibitory activity. 5-farnesyloxy-3-carboxycoumarin demonstrated the most potent inhibitory activity by IC₅₀ = 0.74 μM while 6-farnesyloxy-3-carboxycoumarin was the weakest inhibitor among farnesyl analogs (IC₅₀ = 10.4 μM). Bonding affinity of the designed molecular structures toward 15-LOX-1 3D structure complexed with RS75091, as potent 15-LOX-1 inhibitor, was studied by utilizing docking analysis. There was a direct relationship between lipoxygenase inhibitory potency and prenyl length chain. The ability of the prenyl portion to fill the lipophilic pocket which is formed by Ile663, Ala404, Arg403, Ile400, Ile173 and Phe167 side chains can explain the observed relationship. Similarity rate between the docked models and complexed form of RS75091, from point of view of configuration and conformation, could explain inhibitory potency variation between each prenyloxy substitution of 3-carboxycoumarins.</p></div

General procedure for the synthesis of <i>O</i>-prenylated-3-carboxycoumarins.

<p>General procedure for the synthesis of <i>O</i>-prenylated-3-carboxycoumarins.</p

Inhibitory assessment data of the synthetic compounds in comparison with 4-methyl-2-(4-methylpiperazinyl)pyrimido[4, 5-b]benzothiazine (4-MMPB) against human 15-LOX-1.

<p>The data are shown as ± SD (n = 3).</p

Superimposition of consensus structures of compounds 4c (pink stick) and 4d (green stick) from LFC on RS75091 (yellow stick) in rabbit 15-LOX-1 active site (left). Superimposition of consensus structure of 4d (green stick) from LFC on consensus structure of 4d' (brown stick) from similar cluster (right).

<p>In both figures, Ile663 is not shown.</p

Michaelis-Menten (left) and Lineweaver-Burk (right) plots of human 15-LOX-1 inhibition by 4d.

<p>The Y-intercept average (1/V_max) of the Lineweaver-Burk plot is 376 ± 45 min.Abs^-1 and K_M = 8.73 ± 0.43 μM. The error bars are stated as ± SD (n = 4).</p

Data of the docking analyses results: Ki of the most populated cluster (Ki_MPC), average of all the lowest Ki from each cluster (Ki_LEC), average Ki of all the conformers (Ki_AC) and average Ki of a cluster in which lactone portion of coumarin directed towards Fe-OH core (Ki_LFC).

<p>N = number of conformers. The data are shown as ± SEM.</p