Ex Vitro Rooting and Survival of Regenerated Shoots ‎from three Fig (Ficus carica L.) Genotypes

The aim of this study was to optimize the rooting of regenerated shoots from nodal segments of three genotypes of fig named: ‘Bargchenari’, ‘Dehdez’ and ‘Runu’. For that purpose, the bottom of shoots (about 5 mm) was dipped in Indole-3-butyric acid (IBA) solution of different concentrations: 500, 1000, 1500, 2000 mg L-1 and control for 5 seconds and then cultured in sand medium. The test was performed in a completely randomized design with at least 5 replications. In ‘Runu’, the highest rooting percentage and the number of roots per shoot (100% and 15.67, respectively) were observed in concentration of 2000 mg L-1 ‎IBA, whereas, ‘Bargchenari’ and ‘Dehdez’ produced the highest rooting percentage and the root number per shoot at concentrations of 1000 and 1500 mg L-1 IBA respectively. Three months after transferring rooted shoots to soil mixtures, ‘Bargchenari’ genotype showed the highest survival rate (80%), shoot length (2.5 cm) and leaf number (4.65 leaves per shoot). In relation to survival percentage, the best medium was perlite, but plantlets showed better growth on perlite + peat medium

Ex Vitro Rooting and Survival of Regenerated Shoots ‎from three Fig (Ficus carica L.) Genotypes

The aim of this study was to optimize the rooting of regenerated shoots from nodal segments of three genotypes of fig named: ‘Bargchenari’, ‘Dehdez’ and ‘Runu’. For that purpose, the bottom of shoots (about 5 mm) was dipped in Indole-3-butyric acid (IBA) solution of different concentrations: 500, 1000, 1500, 2000 mg L-1 and control for 5 seconds and then cultured in sand medium. The test was performed in a completely randomized design with at least 5 replications. In ‘Runu’, the highest rooting percentage and the number of roots per shoot (100% and 15.67, respectively) were observed in concentration of 2000 mg L-1 ‎IBA, whereas, ‘Bargchenari’ and ‘Dehdez’ produced the highest rooting percentage and the root number per shoot at concentrations of 1000 and 1500 mg L-1 IBA respectively. Three months after transferring rooted shoots to soil mixtures, ‘Bargchenari’ genotype showed the highest survival rate (80%), shoot length (2.5 cm) and leaf number (4.65 leaves per shoot). In relation to survival percentage, the best medium was perlite, but plantlets showed better growth on perlite + peat medium

Hospital-based prospective study of pertussis in infants and close contacts in Tehran, Iran

International audienceBackground : Pertussis remain a global health concern, especially in infants too young to initiate their vaccination. Effective vaccination and high coverage limit the circulation of the pathogen, yet duration of protection is limited and boosters are recommended during a lifetime. In Iran, boosters are given at 18 months and 6 years old using whole pertussis vaccines for which efficacy is not known, and pertussis surveillance is scant with only sporadic biological diagnosis. Burden of pertussis is not well understood and local data are needed. Methods : Hospital-based prospective study implementing molecular laboratory testing in infants aged ≤6 months and presenting ≥5 days of cough associated to one pertussis-like symptom in Tehran. Household and non-household contact cases of positive infants were evaluated by comprehensive pertussis diagnosis (molecular testing and serology) regardless of clinical signs. Clinical evaluation and source of infection were described. Results : A total of 247 infants and 130 contact cases were enrolled. Pertussis diagnosis result was obtained for 199 infants and 104 contact cases. Infant population was mostly < 3 months old (79.9%; 157/199) and unvaccinated (62.3%; 124/199), 20.1% (40/199) of them were confirmed having B. pertussis infection. Greater cough duration and lymphocyte counts were the only symptoms associated to positivity. Half of the contact cases (51.0%; 53/104) had a B. pertussis infection, median age was 31 years old. A proportion of 28.3% (15/53) positive contacts did not report any symptom. However, 67.9% (36/53) and 3.8% (2/53) of them reported cough at inclusion or during the study, including 20.8% (11/53) who started coughing ≥7 days before infant cough onset. Overall, only five samples were successfully cultured. Conclusion : These data evidenced the significant prevalence of pertussis infection among paucy or poorly symptomatic contacts of infants with pertussis infection. Widespread usage of molecular testing should be implemented to identify B. pertussis infections