Deterministic preparation of a tunable few-fermion system

This thesis reports on the preparation of a tunable few-fermion system using ultracold 6Li atoms in an optical dipole trap. We prepare ground state systems consisting of 1 to 10 fermions with fidelities of ~90%. This system has the unique property that key parameters such as particle number, inter-particle interactions and external confining potential are tunable. We use this model system to explore two interacting atoms confined in a one-dimensional potential. For increasing repulsion we measure a decrease of the tunneling time of one atom through a barrier which is created by tilting the potential. From the measured tunneling time we calculate the interaction energy of the system using the WKB technique. This requires detailed knowledge of the confining potential, which we obtain by controlling the motional quantum state of a single atom in the trap. To increase the preparation fidelity of the few-particle systems a high-resolution objective has been designed during this thesis. It will allow us to explore tunable quantum systems in two and three dimensions confined in arbitrary potentials. Because of its great tunability our model system is uniquely suited to explore strongly correlated few-fermion systems, which is one of the major challenges of modern physics

Radio Frequency Association of Efimov Trimers

The quantum-mechanical three-body problem is one of the fundamental challenges of few-body physics. When the two-body interactions become resonant, an infinite series of universal three-body bound states is predicted to occur, whose properties are determined by the strength of the two-body interactions. We report on the association and direct observation of a trimer state consisting of three distinguishable fermions using radio-frequency (RF) spectroscopy. The measurements of its binding energy are consistent with theoretical predictions which include non-universal corrections.Comment: 12 pages, 6 figure

Together is Better: mRNA Co-Encapsulation in Lipoplexes is Required to Obtain Ratiometric Co-Delivery and Protein Expression on the Single Cell Level

Liposomes can efficiently deliver messenger RNA (mRNA) into cells. When mRNA cocktails encoding different proteins are needed, a considerable challenge is to efficiently deliver all mRNAs into the cytosol of each individual cell. In this work, two methods are explored to co-deliver varying ratiometric doses of mRNA encoding red (R) or green (G) fluorescent proteins and it is found that packaging mRNAs into the same lipoplexes (mingle-lipoplexes) is crucial to efficiently deliver multiple mRNA types into the cytosol of individual cells according to the pre-defined ratio. A mixture of lipoplexes containing only one mRNA type (single-lipoplexes), however, seem to follow the “first come – first serve” principle, resulting in a large variation of R/G uptake and expression levels for individual cells leading to ratiometric dosing only on the population level, but rarely on the single-cell level. These experimental observations are quantitatively explained by a theoretical framework based on the stochasticity of mRNA uptake in cells and endosomal escape of mingle- and single-lipoplexes, respectively. Furthermore, the findings are confirmed in 3D retinal organoids and zebrafish embryos, where mingle-lipoplexes outperformed single-lipoplexes to reliably bring both mRNA types into single cells. This benefits applications that require a strict control of protein expression in individual cells

A minimal-complexity light-sheet microscope maps network activity in 3D neuronal systems

Abstract In vitro systems mimicking brain regions, brain organoids, are revolutionizing the neuroscience field. However, characterization of their electrical activity has remained a challenge as it requires readout at millisecond timescale in 3D at single-neuron resolution. While custom-built microscopes used with genetically encoded sensors are now opening this door, a full 3D characterization of organoid neural activity has not been performed yet, limited by the combined complexity of the optical and the biological system. Here, we introduce an accessible minimalistic light-sheet microscope to the neuroscience community. Designed as an add-on to a standard inverted microscope it can be assembled within one day. In contrast to existing simplistic setups, our platform is suited to record volumetric calcium traces. We successfully extracted 4D calcium traces at high temporal resolution by using a lightweight piezo stage to allow for 5 Hz volumetric scanning combined with a processing pipeline for true 3D neuronal trace segmentation. As a proof of principle, we created a 3D connectivity map of a stem cell derived neuron spheroid by imaging its activity. Our fast, low complexity setup empowers researchers to study the formation of neuronal networks in vitro for fundamental and neurodegeneration research