Using non-smooth multi-domain dynamics to improve the safety on haul roads in surface mining

The paper presents a preliminary numerical study aimed to improve the safety on haul roads in surface mining. The interaction and collision between granular berms and ultra-class haul trucks are investigated by using non-smooth multi-domain dynamics. The haul truck is modelled as a rigid multibody system and the granular berm as a distribution of rigid particles using the discrete element method. A non-smooth dynamics approach is applied to enable stable and time-efficient simulation of the full system with strong coupling. The numerical model is first calibrated using full-scale data from experimental tests and then applied to investigate the collision between the haul truck and granular berms of different geometry under various approach conditions

Direct measurement of TRPV4 and PIEZO1 activity reveals multiple mechanotransduction pathways in chondrocytes

The joints of mammals are lined with cartilage, comprised of individual chondrocytes embedded in a specialized extracellular matrix. Chondrocytes experience a complex mechanical environment and respond to changing mechanical loads in order to maintain cartilage homeostasis. It has been proposed that mechanically gated ion channels are of functional importance in chondrocyte mechanotransduction; however, direct evidence of mechanical current activation in these cells has been lacking. We have used high-speed pressure clamp and elastomeric pillar arrays to apply distinct mechanical stimuli to primary murine chondrocytes, stretch of the membrane and deflection of cell-substrate contacts points, respectively. Both TRPV4 and PIEZO1 channels contribute to currents activated by stimuli applied at cell-substrate contacts but only PIEZO1 mediates stretch-activated currents. These data demonstrate that there are separate, but overlapping, mechanoelectrical transduction pathways in chondrocytes

Resonant interaction between gravitational waves, electromagnetic waves and plasma flows

In magnetized plasmas gravitational and electromagnetic waves may interact coherently and exchange energy between themselves and with plasma flows. We derive the wave interaction equations for these processes in the case of waves propagating perpendicular or parallel to the plasma background magnetic field. In the latter case, the electromagnetic waves are taken to be circularly polarized waves of arbitrary amplitude. We allow for a background drift flow of the plasma components which increases the number of possible evolution scenarios. The interaction equations are solved analytically and the characteristic time scales for conversion between gravitational and electromagnetic waves are found. In particular, it is shown that in the presence of a drift flow there are explosive instabilities resulting in the generation of gravitational and electromagnetic waves. Conversely, we show that energetic waves can interact to accelerate particles and thereby \emph{produce} a drift flow. The relevance of these results for astrophysical and cosmological plasmas is discussed.Comment: 12 pages, 1 figure, typos corrected and numerical example adde

Collagen organization within the cartilage of TRPV4(-/-) mice studied with two-photon microscopy and polarized second harmonic generation

The polymodal channel TRPV4 has been shown to regulate development and maintenance of cartilage. Here we investigate whether TRPV4 activity regulates the early deposition and structure of collagen matrix in the femoral head cartilage by comparing the 3D morphology and the sub-micrometer organization of the collagen matrix between wild type and TRPV4(-/-) mice pups four to five days old. Two-photon microscopy can be used to conduct label-free imaging of cartilage, as collagen generates a second harmonic signal (second harmonic generation [SHG]) under pulsed infrared excitation. In one set of measurements, we use circularly polarized laser light to reconstruct the 3D morphology of the femoral head cartilage and to measure the tissue thickness. Second, by rotating the direction of the linearly polarized light and using polarized SHG detection, we investigate the sub-micrometer orientation of collagen fibers in the cartilage. At this developmental stage, we cannot detect statistically significant differences between the two mice strains, although a tendency toward a more random orientation of collagen fibers and a higher thickness of the whole cartilage seems to characterize the TRPV4(-/-) mice. We discuss possible reasons for these observations

Charged multifluids in general relativity

The exact 1+3 covariant dynamical fluid equations for a multi-component plasma, together with Maxwell's equations are presented in such a way as to make them suitable for a gauge-invariant analysis of linear density and velocity perturbations of the Friedmann-Robertson-Walker model. In the case where the matter is described by a two component plasma where thermal effects are neglected, a mode representing high-frequency plasma oscillations is found in addition to the standard growing and decaying gravitational instability picture. Further applications of these equations are also discussed.Comment: 14 pages (example added), to appear in Class. Quantum Gra

Transverse Wave Propagation in Relativistic Two-fluid Plasmas in de Sitter Space

We investigate transverse electromagnetic waves propagating in a plasma in the de Sitter space. Using the 3+1 formalism we derive the relativistic two-fluid equations to take account of the effects due to the horizon and describe the set of simultaneous linear equations for the perturbations. We use a local approximation to investigate the one-dimensional radial propagation of Alfv\'en and high frequency electromagnetic waves and solve the dispersion relation for these waves numerically.Comment: 19 pages, 12 figure

Cyclotron damping and Faraday rotation of gravitational waves

We study the propagation of gravitational waves in a collisionless plasma with an external magnetic field parallel to the direction of propagation. Due to resonant interaction with the plasma particles the gravitational wave experiences cyclotron damping or growth, the latter case being possible if the distribution function for any of the particle species deviates from thermodynamical equilibrium. Furthermore, we examine how the damping and dispersion depends on temperature and on the ratio between the cyclotron- and gravitational wave frequency. The presence of the magnetic field leads to different dispersion relations for different polarizations, which in turn imply Faraday rotation of gravitational waves.Comment: 15 pages, 3 figures. Accepted for publication in Phys. Rev.

Are your covariates under control? How normalization can re-introduce covariate effects

Many statistical tests rely on the assumption that the residuals of a model are normally distributed. Rank-based inverse normal transformation (INT) of the dependent variable is one of the most popular approaches to satisfy the normality assumption. Studies regularly adjust for covariates and then normalize the residuals. This study investigated the effect of regressing covariates against the dependent variable and then applying rank-based INT to the residuals. The correlation between the dependent variable and covariates at each stage of processing was assessed. An alternative approach was tested of applying rank-based INT to the dependent variable before regressing covariates was tested. Analyses based on both simulated and real data examples demonstrated that applying rank-based INT to the dependent variable residuals after regressing out covariates re-introduces a linear correlation between the dependent variable and covariates in almost all situations. This will increase type-1 errors and reduce power. Our proposed alternative approach, where rank-based INT was applied prior to controlling for covariate effects, gave residuals that were normally distributed and linearly uncorrelated with covariates. This approach is therefore recommended

Modulation of the gut microbiota of Pacific white shrimp ( Penaeus vannamei Boone, 1931) by dietary inclusion of a functional yeast cell wall‐based additive

Several eco-friendly natural substances can enhance the shrimp immune defence system therby acting as a prophylactic agent in feed additives. Agents such as (1, 3)-(1, 6)-D β-glucan and complex mannan-oligosaccharides located in yeast cell walls present such immunomodulatory and potential prebiotic properties. The aim of our study was to evaluate the effect of a commercial yeast cell wall extract (YCW) on shrimp performance and health status, and influence on gut microbiota. Juvenile Penaeus vannamei (Boone, 1931) were raised at an intensive shrimp farm and fed with two different diet inclusions levels of YCW, that is, 0.5% and 1.0%, in addition to a yeast free control group. After 102 days, animals were sampled, and standard nutrition performance parameters were measured. Additionally, the phylogenetic profile and composition of shrimp gut microbiota were evaluated. Animal performance, including growth and survival, was significantly better on animals fed with YCW than the control group. Furthermore, beneficial bacteria phylotypes were stimulated by the presence of YCW, positively modulating the gut microbiota, with emphasis on 1.0% YCW treatment. Therefore, YCW can be regarded as a prophylactic functional agent in the intensive rearing of juvenile P. vannamei thus improving animal performance and contributing to a healthy intestinal microbiota