Agricultural land abandonment in Bulgaria: a long-term remote sensing perspective, 1950–1980

Agricultural land abandonment is a globally significant threat to the sustenance of economic, ecological, and social balance. Although the driving forces behind it can be multifold and versatile, rural depopulation and urbanization are significant contributors to agricultural land abandonment. In our chosen case study, focusing on two locations, Ruen and Stamboliyski, within the Plovdiv region of Bulgaria, we use aerial photographs and satellite imagery dating from the 1950s until 1980, in connection with official population census data, to assess the magnitude of agricultural abandonment for the first time from a remote sensing perspective. We use multi-modal data obtained from historical aerial and satellite images to accurately identify Land Use Land Cover changes. We suggest using the rubber sheeting method for the geometric correction of multi-modal data obtained from aerial photos and Key Hole missions. Our approach helps with precise sub-pixel alignment of related datasets. We implemented an iterative object-based classification approach to accurately map LULC distribution and quantify spatio-temporal changes from historical panchromatic images, which could be applied to similar images of different geographical regions

The role of phosphatidylinositol-3 kinase/AKT and ERK-1/2 pathways in the neuroprotective activity of lithium

..

Phosphorylation of PI3K/Akt at Thr308, but not phosphorylation of MAPK kinase, mediates lithium-induced neuroprotection against cerebral ischemia in mice

Lithium, in addition to its effect on acute and long-term bipolar disorder, is involved in neuroprotection after ischemic stroke. Yet, its mechanism of action is still poorly understood, which was only limited to its modulatory effect on GSK pathway. Therefore, we initially analyzed the dose-dependent effects of lithium on neurological deficits, infarct volume, brain edema and blood-brain barrier integrity, along with neuronal injury and survival in mice subjected to focal cerebral ischemia. Thereafter, we investigated the involvement of the PI3K/Akt and MEK signal transduction pathways and their components. Our observations revealed that 2 mmol/kg lithium significantly improved post-ischemic brain tissue survival. Although, 2 mmol/kg lithium had no negative effect on brain microcirculation, 5 and 20 mmol/kg lithium reduced brain perfusion. Furthermore, supratherapeutic dose of lithium in 20 mmol/kg lead to animal death. In addition, improvement of brain perfusion with L-arginine, did not change the effect of 5 mmol/kg lithium on brain injury. Additionally, post-stroke blood-brain barrier leakage, hemodynamic impairment and apoptosis have been reversed by lithium treatment. Interestingly, lithium-induced neuroprotection was associated with increased phosphorylation of Akt at Thr308 and suppressed GSK-3 beta phosphorylation at Ser9 residue. Lithium upregulated Erk-2 and downregulated JNK-2 phosphorylation. To distinguish whether neuroprotective effects of lithium are modulated by PI3K/Akt or MEK, we sequentially blocked these pathways and demonstrated that the neuroprotective activity of lithium persisted during MEK/ERK inhibition, whereas PI3K/Akt inhibition abolished neuroprotection. Collectively, we demonstrated lithium exerts its post-stroke neuroprotective activity via the PI3K/Akt pathway, specifically via Akt phosphorylation at Thr308, but not via MEK/ERK.Turkish Academy of Sciences (TUBA); Istanbul Medipol UniversityThis research was supported by The Turkish Academy of Sciences (TUBA) and Istanbul Medipol University

Automated Testing of Simulation Software in the Aviation Industry : An Experience Report

An industry-academia collaboration developed a test automation framework for aviation simulation software. The technology has been successfully deployed in several test teams.</p

The protein expression profile of old and young mice after cerebral ischemia

..