Estimation of Inertial Parameters

This report contains a study on inertial parameter estimation for application in robot control. Symbolic manipulation using Maple is applied to support the estimation efforts

Weber, Beck y creación feminista de sentido en la escuela

En las sociedades actuales se ha producido de manera generalizada una pérdida de sentido de las instituciones. Por un lado, a través de algunas de las aportaciones de autores de la teoría sociológica clásica como Weber y contemporánea como Beck, analizamos la pérdida de sentido que se da en las sociedades contemporáneas hacia la institución educativa. Por otro lado, analizamos algunas de las acciones que los sujetos llevan a cabo y que aportan de nuevo sentido a la escuela. Para ello partimos del feminismo dialógico y de las reivindicaciones de las mujeres gitanas que toman la educación como elemento clave. En tanto que sujetos de su propia transformación, las mujeres gitanas se han convertido en el motor de cambio de su propia cultura y en el eje del movimiento feminista gitano

Modelling the effect of prebiotics, probiotics and other functional additives on the growth, feed intake and feed conversion of European sea bass (Dicentrarchus labrax) juveniles

Dietary supplementation of aquafeeds with functional additives is a commonly employed strategy in order to reduce the potential negative effects associated to fishmeal (FM) and fish oil (FO) replacement by alternative protein and oil sources. Nevertheless, the wide variety of functional ingredients with different bioactive properties hinders the selection of appropriate dietary supplementation strategies on feed formulation. The present study aimed to develop an observational multiple-linear regression (MLR) model to identify the effects of a variety of functional ingredients supplementation on European sea bass juveniles (Dicentrarchus labrax) growth performance and feed utilization. A literature survey was conducted gathering a total of 61 dietary treatments. The functional ingredients were classified in three main groups, namely, “probiotics”, “prebiotics” or “others” (including plant derived compounds such as essential oils, extracts and powders). Three different MLR were obtained and validated, allowing to describe the effects of functional ingredients supplementation on fish specific growth rate (SGR) (with a final R-squared (R2) = 0.96, adjusted R-squared (adj R2) = 0.92 and a p-value= 7.21E- 08)), fish feed intake (FI) (R2 = 0.97, adj R2 = 0.95 and a p-value= 5.42E-12)) and fish feed conversion ratio (FCR) (R2 = 0.90, adj R2 = 0.80 and a p-value= 2.02E-05)). MLR model trimming, allowed the detection of a significant positive correlation (cor) between dietary prebiotics supplementation and SGR (cor= 0.32, p-value= 8.52E-04). On the contrary, prebiotic supplementation presented a negative correlation with fish FI (cor= − 0.44, p-value= 6.27E-05) and FCR (cor= − 0.41, p-value= 8.96E-05).This work was founded by the European Union’s Horizon 2020 research and innovation program under grant agreement no. 818367; AquaIMPACT—Genomic and nutritional innovations for genetically superior farmed fish to improve efficiency in European aquaculture. Authors want to thank the University of Las Palmas de Gran Canaria (ULPGC) for the funding for Antonio Serradell through the call “ERASMUS + PRACTICAS ´ (SMT)” 2020-2021.info:eu-repo/semantics/publishedVersio

Dietary phytogenics and galactomannan oligosaccharides in low fish meal and fish oil-based diets for European sea bass (Dicentrarchus labrax) juveniles: effects on gill structure and health and implications on oxidative stress status.

An effective replacement for fish meal (FM) and fish oil (FO) based on plant-based raw materials in the feed of marine fish species is necessary for the sustainability of the aquaculture sector. However, the use of plant-based raw materials to replace FM and FO has been associated with several negative health effects, some of which are related to oxidative stress processes that can induce functional and morphological alterations in mucosal tissues. This study aimed to evaluate the effects of dietary oligosaccharides of plant origin (5,000 ppm; galactomannan oligosaccharides, GMOS) and a phytogenic feed additive (200 ppm; garlic oil and labiatae plant extract mixture, PHYTO) on the oxidative stress status and mucosal health of the gills of juvenile European sea bass (Dicentrarchus labrax). The experimental diets, low FM and FO diets (10%FM/6%FO) were supplemented with GMOS from plant origin and PHYTO for 63 days. GMOS and PHYTO did not significantly affect feed utilization, fish growth, and survival. GMOS and PHYTO downregulated the expression of b-act, sod, gpx, cat, and gr in the gills of the fish compared with that in fish fed the control diet. The expression of hsp70 and ocln was upregulated and downregulated, respectively, in the GMOS group compared with that in the control group, whereas the expression of zo-1 was downregulated in the PHYTO group compared with that in the GMOS group. The morphological, histopathological, immunohistochemical, and biochemical parameters of the fish gills were mostly unaffected by GMOS and PHYTO. However, the PHYTO group had lower incidence of lamellar fusion than did the control group after 63 days. Although the tissular distribution of goblet cells was unaffected by GMOS and PHYTO, goblet cell size showed a decreasing trend ( 1211%) in the GMOS group. GMOS and PHYTO significantly reduced the concentration of PCNA+ in the epithelium of the gills. The above findings indicated that GMOS and PHYTO in low FM/FO-based diets protected the gill epithelia of D. labrax from oxidative stress by modulating the expression of oxidative enzyme-related genes and reducing the density of PCNA+ cells in the gills of the fish

Dietary phytogenics and galactomannan oligosaccharides in low fish meal and fish oil-based diets for European sea bass (Dicentrarchus labrax) juveniles: Effects on gut health and implications on in vivo gut bacterial translocation

European sea bass were fed four low FM/FO (10%/6%) diets containing galactomannan oligosaccharides (GMOS), a mixture of garlic oil and labiatae plants oils (PHYTO), or a combination of both functional products (GMOSPHYTO) for 63 days before exposing the fish to an intestinal Vibrio anguillarum infection combined with crowding stress. In order to evaluate functional diets efficacy in terms of gut health maintenance, structural, cellular, and immune intestinal status were evaluated by optical and electron microscopy and gene expression analyses. A semi-automated software was adapted to determine variations in goblet cell area and mucosal mucus coverage during the challenge test. Feeding with functional diets did not affect growth performance; however, PHYTO and GMOS dietary inclusion reduced European sea bass susceptibility to V. anguillarum after 7 days of challenge testing. Rectum (post-ileorectal valve) showed longer (p = 0.001) folds than posterior gut (pre-ileorectal valve), whereas posterior gut had thicker submucosa (p = 0.001) and higher mucus coverage as a result of an increased cell density than rectum. Functional diets did not affect mucosal fold length or the grade of granulocytes and lymphocytes infiltration in either intestinal segment. However, the posterior gut fold area covered by goblet cells was smaller in fish fed GMOS (F = 14.53; p = 0.001) and PHYTO (F = 5.52; p = 0.019) than for the other diets. PHYTO (F = 3.95; p = 0.049) reduced posterior gut goblet cell size and increased rodlet cell density (F = 3.604; p = 0.068). Dietary GMOS reduced submucosal thickness (F = 51.31; p = 0.001) and increased rodlet cell density (F = 3.604; p = 0.068) in rectum. Structural TEM analyses revealed a normal intestinal morphological pattern, but the use of GMOS increased rectum microvilli length, whereas the use of PHYTO increased (p 640.10) Ocln, N-Cad and Cad-17 posterior gut gene expression. After bacterial intestinal inoculation, posterior gut of fish fed PHYTO responded in a more controlled and belated way in terms of goblet cell size and mucus coverage in comparison to other treatments. For rectum, the pattern of response was similar for all dietary treatments, however fish fed GMOS maintained goblet cell size along the challenge test

Genotype x nutrition interactions in European sea bass (Dicentrarchus labrax): effects on gut health and intestinal microbiota

One of the main objectives of the present study was an effective replacement of dietary fishmeal/fish oil (FM/FO) by new raw materials without negatively affecting European sea bass (Dicentrarchus labrax) performance and health status within a selection breeding context. The genomic selection of this species is still in its infancy if compared to salmonids and in particular, the role of D.labrax genome in shaping the gut microbiome, has been scarcely investigated. Wildtype (WT) and selected (GS) sea bass were fed with two diets: a control (20% FM/ 5–9% FO), and a “future” (F) diet, in which FM was decreased to 10% being replaced by poultry meal, whereas FO was completely replaced by a blend of rapeseed, poultry, and algae oils. The morphological evaluation of the intestine revealed a well-organized folding pattern and a conserved gut epithelial barrier for all fish groups. Despite a basal level of inflammation in the proximal intestine of WT fish, no differences were observed neither in the morphometric characteristics of goblet cells nor in the expression of GALT-related genes in response to fish genotype or diet. At distal intestine, WT fish showed a higher inflammatory status and larger goblet cells than GS fish and within the same genotype, fish fed the F diet had in general larger goblet cells. In distal intestine, a significant effect was found on the expression of 3 out of 7 target GALT genes. In particular, the expression of cytokines il-1β, tnf-α, and il-10 was different, showing an interaction effect diet x genotype. Diet had a lower influence upon gut bacterial composition than genotype. Indeed, regardless of the diet, WT fish showed higher species richness than GS genotype and this could be a direct consequence of selective breeding for multiple traits selection including growth, external morphology for lower abdominal fat deposition and adaptation to multiple and successive feed sources and composition across generations of selection. Furthermore, the gut microbiota of GS fish shared a reduced individual variability, indicating an enhanced capacity to cope with changes in diet composition. The less changes of GS sea bass at the level of gut bacterial composition in cumulating data collected with the feeds, demonstrate a capacity to reshape their microbiota thus better adapting to the diet, but with no negative impact on their growth performances, and even a better growth. A significant genotype effect was found for specific bacterial taxa, such as Paracoccus genus and other genera belonging to Moraxellaceae family, which were enriched in WT fish, regardless of the diet. Interestingly, the relative abundance of Paracoccus genus was positively correlated with higher proinflammatory cytokine il-1β expression found in distal intestine of wildtype sea bass

Functional additives in a selected european sea bass (Dicentrarchus labrax) genotype: effects on the stress response and gill antioxidant response to hydrogen peroxide (H2O2) treatment.

Husbandry practices in aquaculture production may lead to stress processes and oxidative stress damages on fish tissues. Functional ingredients have profiled as suitable candidates for reinforcing the fish antioxidant response and stress tolerance. In addition, selective breeding strategies have also demonstrated a correlation between fish growth and stress reactiveness, which may be a key component in species domestication. The present study evaluates the potential of three different functional additives for gill endogenous antioxidant capacity and stress relief in a growth selected genotype of European sea bass (Dicentrarchus labrax) juveniles fed low-FM/FO diets. For this purpose, after 72 days of a feeding trial, all fish were subjected to an oxidative stress challenge consisting of a 1 h bath exposure to hydrogen peroxide (H2O2 ) at a total concentration of 50 ppm. The functional additives induced a better recovery from the stress process, with a higher reduction in fish circulating plasma cortisol 24 h after oxidative stress. In addition, the functional additives induced higher catalase gill gene expression in response to the oxidative stress insult

Nutritional innovations in superior European sea bass (Dicentrarchus labrax) genotypes: implications on fish performance and feed utilization.

The sustainable development of the aquaculture industry relies on the use of alternative conventional and emergent raw materials that contributes to a circular economy and to reduce the dependency on fish meals and fish oils coming from oceanic fish populations. Additionally, the genetic selection of farmed fish that can display higher growth and feed utilization when fed alternative feeds, is pointed out to be a complementary valuable tool to facilitate the implementation of circular economy approaches. The main purpose of the present study was to determine the effectiveness of genetic selection for growth in European sea bass, in response to a challenge with an alternative diet that aimed to partially replaced fishmeal (FM) by poultry meal (PM) and totally replace fish oil (FO) by a blend of poultry oil (PO) with a novel microalgae oil. The two families of fish juveniles were obtained by in vitro fertilization of selected for a multi-trait including high growth (genetically selected, GS) or nonselected (wild type, WT) broodstocks and then were nutritionally challenged with a control diet that mirrored a standard commercial diet with fishmeal (20%) and fish oil (7%), or a Future diet that partially replaced the FM by PM and totally replaced the FO by a blend of rapeseed oil, PO, and a novel DHA rich-algal oil. From the second month of feeding until the end of the trial, European sea bass that was selected since for 7 generations performed better in terms of growth than the wild-type genotype, possibly related with an apparent favored feed and nutrient utilization. Furthermore, selection decreased the perivisceral fat and increased the nutritional value of flesh by increasing DHA (in g/ 100 g flesh) and ARA contents. In contrast, the dietary treatment showed little effect on fish growth performance, denoting the successful partial replacement of FM by PM and the total replacement of FO by a blend of poultry oil and an emergent microalgal oil. However, Future diet tended to reduce the ADCs of some amino acids, as well as showed an additive effect to genotype in increasing the n-3 PUFA of flesh. Altogether, our data demonstrate that multi-trait genetic selection of European sea bass improve fish plasticity to cope with the variations of ingredients in alternative feeds with low FM/FO

Caracterización y actividad catalítica de bionanocatalizadores de platino soportados sobre sistemas proteínas de capa-s/poliuretano

Las subunidades de proteínas de capa-S poseen la capacidad de autoensamblarse sobre distintas superficies formando arreglos en la escala nanométrica. Este aspecto disparó el interés por el empleo de estas proteínas en la construcción biomolecular con prometedoras aplicaciones nanobiotecnológicas