Anti-oxidant, DNA-damage protection and anti-cancer properties of n-butanol extract of the endemic Perralderia coronopifolia

This study was designed to evaluate in vitro the total phenolic, flavonoid content, anti-oxidant activity, oxidative DNA-damage protection and anti-cancer activity of n-butanol extract from Perralderia coronopifolia, endemic plant. DNA protection capacity was performed using 46966 plasmid DNA against UV-photolysis of H2O2-induced oxidative damage. The anti-prolifer-ative effects of extract were performed on HeLa and C6 cells. Experimental results showed that extract had convenient number of phenolic and flavonoids. Furthermore, it provided strong anti-oxidant, reducing power, hydrogen peroxide and higher DPPH· scavenging ability with IC50= 7.02 ± 0.02 µg/mL. As it shown an oxidative plasmid DNA-damage inhibition against UV-photolysis of H2O2, an anti-cancer activity athigher concentrations in cells. The data obtained that P. coronopifolia extract could be useful in human protection against infection and degenerative illness. Video Component of Methodology: Metal chelating activity:   2 min 56 sec   Full Screen   Alternat

Chemical constituents, in vitro antioxidant and antiproliferative activities of Perralderia coronopifolia Coss. subsp. eu-coronopifolia M. var. typica M. extract

Phytochemical investigations of extracts from the aerial parts (leaves and flowers) of Perralderia coronopifolia Cosson resulted in the isolation of nine secondary metabolites corresponding to three flavonoids: rhamnazin, chrysosplenol D, and (2R, 3R) taxifolin, two monoterpene glycosides: myrtenol- β-Dglucopyranoside- 6'-O-acetate and myrtenol β-D-glucopyranoside, a disaccharide: sucrose and three di-Ocaffeoylquinic acid derivatives : methyl 3, 5-di-O-caffeoyl quinate and methyl 3,4-di-O-caffeoyl quinate as a mixture and 1,5-di-O-caffeoylquinic acid. The structures were identified by spectroscopic methods such as 1H and 13C NMR, COSY, HSQC and HMBC experiments, HRESI-MS and comparison with literature data. Myrtenol-β-Dglucopyranoside-6'-O-acetate was isolated in pure and native state for the first time. The other compounds are new for the genus Perralderia Cosson. The ethyl acetate extract showed a high antioxidant effect, especially DPPH radical scavenging activity with IC50=7.01±0.28µg/mL) compared to ascorbic acid (IC50= 5±0.1µg/mL. This extract also showed antiproliferative activity against HeLa (human cervix carcinoma) and C6 (rat brain tumor) cells.Peer Reviewe