Advanced multimodal laser imaging tool for urothelial carcinoma diagnosis (AMPLITUDE)

Bladder cancer (BC) is the eleventh most diagnosed cancer worldwide. The age-standardized incidence rate (per 100 000 person/years) is 9.0 for men and 2.2 for women [1]. Urothelial carcinoma (UC) represents about 90% of all bladder tumors, thus carrying an enormous social and economic burden [2]. UCs are classified in different stages and grades, depending on their invasiveness and on their degree of cytological abnormalities. The key aspect for a positive prognosis is the early and accurate diagnosis of the lesion stage, in order to identify the most aggressive disease forms and treat them promptly. It is well known that tissue metabolism constitutes a basic mechanism, which is at the base of many pathologies, especially BC. Being able to detect and characterize tissue metabolism and molecular fingerprints at the cellular level could be a key aspect in characterizing the pathology and enabling both early detection and therapy monitoring. The new European Union Horizon 2020 project called AMPLITUDE, the ‘Advanced Multimodal Photonics Laser Imaging Tool for Urothelial Diagnosis in Endoscopy’, starting in January 2020, proposes the development of an advanced multi-modal imaging tool exploiting new laser technologies in an approach combining confocal and non-linear imaging to fulfil unmet clinical needs in terms of the specificity and accuracy of urothelial cancer diagnosis and therapy monitoring. The project is coordinated by Tampere University (Finland) and carried out in cooperation with leading European research organizations including Aston Insitute of Photonic Technologies—AIPT (UK), Consiglio Nazionale delle Ricerche—CNR (Italy), Institute of Photonic Sciences—ICFO (Spain), University of Milan-Bicocca, Modus Research and Innovation Ltd. (UK) and University of Florence (Italy), as well as industrial partners: Ampliconyx Oy (Finland), Femtonics Ltd. (Hungary), HC Photonics (Taiwan), and LEONI Fiber Optics GmbH (Germany)

Improving the level of physical development and functional preparedness athletes in sports dancing on the stage of specialized basic training means step aerobics

Purpose: make analyze the impact of the step aerobics on the indicators of physical development and functional preparedness of athletes in Dance Sport on the stage of specialized basic training. Material & Methods: 20 athletes (10 sport duets) aged 14–15 years were divided into two groups: control (CG) and experimental (EG). To achieve the objectives we used methods: theoretical - analysis and synthesis data of scientific and methodological literature; pedagogical methods: pedagogical observation; pedagogical testing; medical and biological methods: anthropometry, functional methods of research; methods of mathematical statistics. Results: after the implementation of a training process means step aerobics significantly improved indicators of cardio-respiratory system of athletes in Dance Sport. Conclusions: materials research to assess allow us to estimate the extent of influence of step aerobics on indicators of physical development and functional preparedness athletes in Dance Sport on the stage of specialized basic training

Herpes simplex virus 1 infection induces morphological changes in brain and liver in BALB/c mice

Introduction: Herpes simplex virus type I (HSV-I) is quite prevalent in the general population. HSV-I exists in a latent form in the nervous system. The effects on other organs, particularly the liver, are not studied enough. The aim of the current work is to study morphological features of systemic or­gan injury caused by HSV-I.Materials and methods: Experiments were conducted on BALB/c line mice weighing 18-20g. The an­imals were infected with mouse-adapted HSV-I in the Institute of Epidemiology and Infectious Dis­eases (Kyiv, Ukraine). On day 30 histological studies of the brain and liver were conducted. The virus in blood serum, brain and liver was assessed by polymerase chain reaction (PCR) and dot enzyme-linked immunosorbent assay (dot-ELISA).Results: Using PCR, dot-ELISA and histological methods the presence of HSV-I and organ damage was confirmed in 100% of samples. Focal infiltration of lymphocytes and monocytes in mice brains was observed in the corpus callosum, brain cortex and hippocampus. The inflammation caused neu­rodegenerative changes leading to reduction of neurons in the studied areas. Herpes infection in liv­er was marked by hyperaemia of hemocapillaries and central veins and local hemorrhages. Some por­tal tracts demonstrated focal accumulation of neutrophils and monocytes. Cytopathological changes (cell dystrophy, hypertrophic nuclei) of hepatocytes were focal or diffuse. The patterns of herpetic liv­er infection are characterized by the absence of tissue basophil response and their absence in the stro­ma as well as by the delayed process of fibrosis.Conclusions: The experimental data showed that HSV-infection is not limited to the neurodegenera­tive changes in the nervous system and can cause systemic damage of organs. Our study demonstrat­ed features of systemic organ damage due to HSV-I viremia. This data also suggests a spread and pen­etration of the infection into the organs following viral damage of the blood vessel wall

Application of multi-layer chitosan-based wound dressings in dentistry

Aim. To experimentally evaluate the efficacy of multi-layer chitosan-based wound dressings developed for dental purposes.Materials and Methods. Over the period from 2018 to 2019, an experimental study was conducted to evaluate the efficacy of multi-layer chitosan-based wound dressings developed for dental practice. Two types of dressings (No. 1c and 2c), the central part of which was modified with the introduction of a 10% iodopyron solution, were compared with the widely-used wound dressings Alvostaz (NKF Omega-Dent LLC, Russia) and Gelatamp (Roeko, Germany). The work involved 26 male Chinchilla rabbits, ten months old and weighing 2500 (±50) g. Evaluation of the efficacy of the wound dressings under study was carried by modelling the conditions of both aseptic and purulent-inflammatory processes (a total of 8 series of experiments). In experiments modelling the purulent-inflammatory process, a Ps. aeruginosa bacterial culture at a concentration of 109 CFU/ml was introduced into the alveolar socket after tooth extraction, 3 days before the application of a wound dressing sample. The dressings under study were applied on the wound for the period of 7 days. During the observation postoperative period, the animals were provided with free access to water and food. The sampling of the material for the study was carried out in the operating room on day 7.Results. According to the experimental results, chitosan-based wound dressings (No. 2c in particular) demonstrated high efficacy in experiments on animals (rabbits). The developed wound dressings perform mechanical protection and exhibit fuse effects, as well as are characterized by biodegradability and the ability to deliver medical agents contained therein to the damaged tissue.Conclusion. The proposed chitosan-based wound dressings showed sufficient efficiency on the modelled wound process in the alveolar socket of an extracted tooth. Dressing No. 2c was recognised to be optimal for infected and purulent wounds. This dressing has a dense outer layer, which structure retains frame functions for a long time, thus providing proper drainage of the pathological focus. Dressing No. 1c was established to be promising in cases without inflammatory processes in the extracted tooth socket, where high adhesion to surrounding tissues is initially required for ensuring the tightness and preservation of a blood clot in the wound

Development of herpetic infection associated with stroke and its correction with acyclovir

Background: Herpes simplex virus (HSV) is prevalent in today’s world population, and there is evidence of potential HSV reactivation in patients with immune deficiency induced by acute stroke. However, the data on the use of antivirals in the setting of stroke are scarce. The aim of this study was to evaluate the reactivation of HSV-1 in patients with stroke, using several methods, and to assess the efficacy of acyclovir in the treatment of experimental stroke. In the employed methodology, PCR and dot-ELISA were used to detect the occurrence of HSV-1 in patients with acute stroke. White mice were infected with HSV-1 and experimental stroke was simulated. The infected mice with stroke were subdivided into two groups: one of them received no treatment, while the other one was treated with acyclovir. The level of HSV-1 reactivation was determined by the methods used in human patients. The brain tissue of experimental animals was also subjected to morphological and morphometrical study. The results of such work reveal that, by the applied serological method, HSV-1 was found in all patients with stroke. Herein, the increased level of HSV-1 was seen in the brain tissue and blood in 100% of the experimental infected animals. However, the use of acyclovir suppressed reproduction of HSV-1. Hence, it can be concluded that clinical and laboratory studies have demonstrated the different sensitivity of Dot-Elisa and PCR, with the former being more sensitive. Moreover, the use of acyclovir in the experiment inhibited viral reproduction and further development of viral infection. Still, chemic lesions in the brain persisted

Abstracts of the First Eurasian Conference; The Coronavirus Pandemic and Critical ICT Infrastructure

While the world is struggling with COVID-19, the ICT industry seeks to play a constructive role in combating the spread of the virus. This book contains the abstracts of the papers presented at The First Eurasian Conference; The Coronavirus Pandemic and Critical ICT Infrastructure (PANDEMIC-ICT-2020) organized by AMIR Technical Services LLC, Tbilisi, Georgia held on November 28-30, 2020. Conference Title: The Coronavirus Pandemic and Critical ICT InfrastructureConference Acronym: PANDEMIC-ICT-2020Conference Date: 28-30 November 2020Conference Location: Online (Virtual Mode)Conference Organizer: AMIR Technical Services LLC, Tbilisi, Georgi