The Order Calculation in the Engineering Company

Import 23/07/2015Diplomová práce je zpracovávána ve společnosti Hagemann a.s. Celá práce se zabývá kalkulací veřejné zakázky Ministerstva vnitra ČR na dodání speciálně upravených policejních vozidel pro monitoring reálných hrozeb. Diplomová práce je rozdělena na dvě základní části, a to teoretickou část a praktickou část. První z částí je popsána problematika veřejných zakázek a hospodářská činnost v podniku. V druhé části je proveden rozbor předmětu veřejné zakázky. Dále byl zpracován návrh postupu plnění zakázky v prostorách společnosti, na které navazuje cenová kalkulace. V závěru práce jsou výsledky práce vyhodnoceny.Master thesis is processed in the company Hagemann a.s. The thesis deals with the calculation of public procurement from Ministry of the Interior to supply police vehicles specially adapted for monitoring real threats. The thesis is divided into two parts, the theoretical and practical part. The first part describes the issue of public procurement and economic activities in the company. The second part is analyzing subject of the contract. Further was processed a proposal for the execution of the contract in the company's premises, which is followed by a calculation and types of costs. In conclusion, the results of the work are evaluated.345 - Katedra mechanické technologievýborn

Genetic Structure of Wild Bonobo Populations: Diversity of Mitochondrial DNA and Geographical Distribution

<div><p>Bonobos (<i>Pan paniscus</i>) inhabit regions south of the Congo River including all areas between its southerly tributaries. To investigate the genetic diversity and evolutionary relationship among bonobo populations, we sequenced mitochondrial DNA from 376 fecal samples collected in seven study populations located within the eastern and western limits of the species’ range. In 136 effective samples from different individuals (range: 7–37 per population), we distinguished 54 haplotypes in six clades (A1, A2, B1, B2, C, D), which included a newly identified clade (D). MtDNA haplotypes were regionally clustered; 83 percent of haplotypes were locality-specific. The distribution of haplotypes across populations and the genetic diversity within populations thus showed highly geographical patterns. Using population distance measures, seven populations were categorized in three clusters: the east, central, and west cohorts. Although further elucidation of historical changes in the geological setting is required, the geographical patterns of genetic diversity seem to be shaped by paleoenvironmental changes during the Pleistocene. The present day riverine barriers appeared to have a weak effect on gene flow among populations, except for the Lomami River, which separates the TL2 population from the others. The central cohort preserves a high genetic diversity, and two unique clades of haplotypes were found in the Wamba/Iyondji populations in the central cohort and in the TL2 population in the eastern cohort respectively. This knowledge may contribute to the planning of bonobo conservation.</p> </div

Relation between genetic distance (F_ST) and geographical indices.

<p>Each pair of seven populations, in all 21 pairs, is dotted as a different symbol according to combination of cohorts. (a) Geographical distance between two populations was measured as a straight line. (b) Geographical distance was measured by detouring headwater of big tributaries or lakes. (c) Number of tributaries on the straight-line between two populations. *p<0.05, ***p<0.001.</p

Genetic diversity for each cohort.

<p>Genetic diversity for each cohort.</p

Calculations of AIC using GLM for single factor models.

<p>F_ST was used as a response variable and Gaussian (identity) was used as a family (link function). Signs in parenthesis mean direction to increase F_ST.</p

Possible ancestral ranges (%) for each node in the phylogenetic tree indicated by RASP.

<p>Possible ancestral ranges (%) for each node in the phylogenetic tree indicated by RASP.</p

Genetic diversity of mtDNA haplotypes within seven populations of bonobos in DRC.

<p>Genetic diversity of mtDNA haplotypes within seven populations of bonobos in DRC.</p

Calculations of AIC using GLM for two-factor models.

<p>F_ST was used as a response variable and Gaussian (identity) was used as a family (link function). Signs in parenthesis mean direction to increase F_ST.</p

Comparison of geographical structure of populations by assessments with AMOVA.

<p>Comparison of geographical structure of populations by assessments with AMOVA.</p

Genetic distances among three cohorts.

<p>Three circles mean east, central and west cohort respectively. Numeric values in double-headed arrows indicate F_ST distances among three cohorts calculated by UPGMA (the left tree).</p