Vibrational Spectroscopies and Chemometry for Nondestructive Identification and Differentiation of Painting Binders

A comprehensive dataset of vibrational spectra of different natural organic binding media is presented and discussed. The binding media were applied on a glass substrate and analyzed after three months of natural ageing. The combination of Raman and FT-NIR spectroscopies allows for an improved identification of these materials as Raman technique is more informative about the skeletal vibrations, while FT-NIR spectroscopy is more sensitive to the substituents and polar groups. The experimental results are initially discussed in the framework of current spectral assignment. Then, multivariate analysis (PCA) is applied leading to differentiation among the samples. The two major principal components allow for a complete separation of the different classes of organic materials. Further differentiation within the same class is possible thanks to the secondary components. The loadings obtained from PCA are discussed on the basis of the spectral assignment leading to clear understanding of the physical basis of this differentiation process

Studio mediante spettroscopia in riflettanza non invasiva dei pigmenti utilizzati dal pittore Federico Zandomeneghi sul dipinto "Bastimento allo scalo" (Galleria d’Arte Moderna di Firenze Palazzo Pitti)

La tecnica impiegata per la caratterizzazione del materiale pittorico si basa sull’analisi di spettri di riflettanza acquisiti in maniera non invasiva. Con questo termine (spettri di riflettanza), si definiscono grafici in cui l’intensità della radiazione retro-diffusa dalla superficie investigata è riportata sull’asse delle ordinate in funzione della lunghezza d’onda della radiazione inviata sull’area di misura. Il valore dell’intensità, riportato come percentuale di luce diffusa (riflessa) dalla superficie investigata, è rapportato ad un bianco di riferimento, che si assume diffondente la radiazione incidente al 100% su tutto l’intervallo spettrale considerato

Benefits of glucocorticoids in non-ambulant boys/men with Duchenne muscular dystrophy: A multicentric longitudinal study using the Performance of Upper Limb test

The aim of this study was to establish the possible effect of glucocorticoid treatment on upper limb function in a cohort of 91 non-ambulant DMD boys and adults of age between 11 and 26 years. All 91 were assessed using the Performance of Upper Limb test. Forty-eight were still on glucocorticoid after loss of ambulation, 25 stopped steroids at the time they lost ambulation and 18 were GC naive or had steroids while ambulant for less than a year. At baseline the total scores ranged between 0 and 74 (mean 41.20). The mean total scores were 47.92 in the glucocorticoid group, 36 in those who stopped at loss of ambulation and 30.5 in the naive group (p <0.001). The 12-month changes ranged between -20 and 4 (mean -4.4). The mean changes were -3.79 in the glucocorticoid group, -5.52 in those who stopped at loss of ambulation and -4.44 in the naive group. This was more obvious in the patients between 12 and 18 years and at shoulder and elbow levels. Our findings suggest that continuing glucocorticoids throughout teenage years and adulthood after loss of ambulation appears to have a beneficial effect on upper limb function. (C) 2015 The Authors. Published by Elsevier B.V

The clinical effectiveness of an integrated multidisciplinary evidence-based program to prevent intraoperative pressure injuries in high-risk children undergoing long-duration surgical procedures: a quality improvement study

The prevention of hospital-acquired pressure injuries (HAPIs) in children undergoing long-duration surgical procedures is of critical importance due to the potential for catastrophic sequelae of these generally preventable injuries for the child and their family. Long-duration surgical procedures in children have the potential to result in high rates of HAPI due to physiological factors and the difficulty or impossibility of repositioning these patients intraoperatively. We developed and implemented a multi-modal, multi-disciplinary translational HAPI prevention quality improvement program at a large European Paediatric University Teaching Hospital. The intervention comprised the establishment of wound prevention teams, modified HAPI risk assessment tools, specific education, and the use of prophylactic dressings and fluidized positioners during long-duration surgical procedures. As part of the evaluation of the effectiveness of the program in reducing intraoperative HAPI, we conducted a prospective cohort study of 200 children undergoing long-duration surgical procedures and compared their outcomes with a matched historical cohort of 200 children who had undergone similar surgery the previous year. The findings demonstrated a reduction in HAPI in the intervention cohort of 80% (p &lt; 0.01) compared to the comparator group when controlling for age, pathology, comorbidity, and surgical duration. We believe that the findings demonstrate that it is possible to significantly decrease HAPI incidence in these highly vulnerable children by using an evidence-based, multi-modal, multidisciplinary HAPI prevention strategy

Valuing unfamiliar Mediterranean deep-sea ecosystems using visual Q-methodology

Monetary valuation of non-market environmental goods and services such as marine ecosystems is a difficult task, usually approached by stated preference methods. Valuation results are often unstable since preferences for unfamiliar, often highly abstract and complex environmental goods depend on the level of previous knowledge of the participant stakeholders and the information provided to them. In this paper, Q methodology has been applied to explore subjective perspectives on Mediterranean deep-sea, which is among the least explored environment in the world. The participant sample consisted in eight Ph.D. students, half of which with a Marine Life Sciences degree. They were asked to perform a Q sorting experiment, and rank a Q sample of thirty-six underwater photographs of the marine wildlife, landscapes, and ecosystems in the Mediterranean deep sea. Photographs were sorted by each topic according to a subjective priority relative to: a) a personal overall view; b) their perception of the potential interest for fishermen; and c) as if they were fishermen. The study revealed three distinct discourses on the subjective importance of deep-sea ecosystems in the Mediterranean Sea: “Noah's Ark Fans”, “Ecosystem Functions Supporters” and “Deep Coral Lovers”. Data analysis showed that the main differences between factors could be related to the experience and the cultural background of the participants. This study improved our knowledge about individuals’ perceptions on Mediterranean deep-sea ecosystems and represents a preliminary step for their monetary valuation

Estimating preferences for Mediterranean deep-sea ecosystem services: a discrete choice experiment

The deep sea represents Earth’s largest (but least explored) biome. It is increasingly affected by anthropogenic stressors and climate change, which threaten the provision of essential ecosystem services. The monetary value of these benefits has rarely been assessed. High biodiversity is hosted in the deep sea and, more generally, in the oceans. This paper uses a hypothetical choice experiment to investigate Italian households’ preferences for deep- sea ecosystem services. The data show wide heterogeneity of the preferences for preserving the Mediterranean deep sea. Many respondents indicate that they would refuse to pay to support the protection of biodiversity and scientific research in this remote and unfamiliar environment. Overall, global warming was of little concern for most respondents, who would not be willing to pay to limit the increase in global temperatures. High income and formal education positively influenced the willingness of the respondents to donate to Non-Governmental Organization’s initiatives to support the Mediterranean deep sea. Deep-water corals appear more ‘charismatic’ to respondents than submarine canyons among deep-sea habitats

Is erythropoietin gene a modifier factor in amyotrophic lateral sclerosis?

To investigate the role of erythropoietin (EPO) as genetic determinant in the susceptibility to sporadic amyotrophic lateral sclerosis (SALS). We sequenced a 259-bp region spanning the 3'hypoxia-responsive element of the EPO gene in 222 Italian SALS patients and 204 healthy subjects, matched for age and ethnic origin. No potentially causative variation was detected in SALS subjects; in addition, two polymorphic variants (namely C3434T and G3544T) showed the same genotype and haplotype frequencies in patients and controls. Conversely, a weak but significant association between G3544T and age of disease onset was observed (p=0.04). Overall, our data argue against the hypothesis of EPO as a genetic risk factor for motor neuron dysfunction, at least in Italian population. However, further studies on larger cohort of patients are needed to confirm the evidence of EPO gene as modifier factor

Long term natural history data in ambulant boys with duchenne muscular dystrophy: 36-month changes

The 6 minute walk test has been recently chosen as the primary outcome measure in international multicenter clinical trials in Duchenne muscular dystrophy ambulant patients. The aim of the study was to assess the spectrum of changes at 3 years in the individual measures, their correlation with steroid treatment, age and 6 minute walk test values at baseline. Ninety-six patients from 11 centers were assessed at baseline and 12, 24 and 36 months after baseline using the 6 minute walk test and the North Star Ambulatory Assessment. Three boys (3%) lost the ability to perform the 6 minute walk test within 12 months, another 13 between 12 and 24 months (14%) and 11 between 24 and 36 months (12%). The 6 minute walk test showed an average overall decline of -15.8 (SD 77.3) m at 12 months, of -58.9 (SD 125.7) m at 24 months and -104.22 (SD 146.2) m at 36 months. The changes were significantly different in the two baseline age groups and according to the baseline 6 minute walk test values (below and above 350 m) (p<0.001). The changes were also significantly different according to steroid treatment (p = 0.01). Similar findings were found for the North Star Ambulatory Assessment. These are the first 36 month longitudinal data using the 6 minute walk test and North Star Ambulatory Assessment in Duchenne muscular dystrophy. Our findings will help not only to have a better idea of the progression of the disorder but also provide reference data that can be used to compare with the results of the long term extension studies that are becoming available