Additional education in the system of professional training of university students

The article reflects the results of the implementation of a theoretical and experimental approach to understanding the role of additional education in the professional development of university students. Considering additional education as an element of the continuing education system, we believe that it is the activation of the development of additional education programs for university students, as well as the creation of conditions for effective implementation in the educational process, that will allow us to realize the main task of further education, as defined in the Law of the Russian Federation “On Education”, - education of a creative person, ready for self-development and self-determination. At the same time, the study we organized showed that only a little more than 50% of the students who took part in our study are positively oriented towards receiving additional education. In order to stimulate students' interest in obtaining additional education, including, within the framework of optimizing their professional development, we propose to focus on “immersing” students in research activities

The Effect of the Implementation of E-Invoice 3.0 and Taxable Person Compliance to Value Added Tax Revenue at KPP Pratama Pasar Rebo

Compliance of Taxable Person (PKP) is a complicated problem related to the implementation of an e-invoice application, especially the knowledge and perceptions of those who consider that making electronic-based tax invoices is difficult to do. This study aims to determine and analyze the effect of the e-invoice 3.0 application and Taxable Person compliance on the increase in VAT revenues. To obtain the research sample, the author used a non-probability sampling technique with the convenience sampling method. The results showed that e-invoice 3.0 implementation and Taxable person compliance simultaneously had a positive and significant effect on VAT revenues with a value of 113,819. The author provides suggestions for the Primary Tax Office of Pasar Rebo, Jakarta to continue improving its services in providing information and campaign related to the use of e-invoice 3.0 to Taxable person to improve their compliance and also to increase the amount of tax revenue which can affect VAT revenues

Innovational methods of development of intellectual labor for economy’s security

The notion “development of intellectual labor for the purpose of economy’s security” is viewed as development of society’s intellectual potential that includes the protected socio-economic information, developed by a person or a group of persons. The social factors that reduce economic security and their consequences in economy are given, namely: negative dynamics of implementing new progressive technologies into production, insufficient coordination of work in the sphere of innovational development, etc. The forms of intellectual development of human resources (intellectual development of personality, control over intellectual information) are offered, which bring the country’s economy to competitiveness and security. The traditional and innovational methods of intellectual labor development are studied (studying in universities and colleges, increase of personnel’s qualification in view of academic degrees (Ph.D., doctor of economics), as well as receipt of economic information through Internet resources, scientific publication, statistical information, etc.), as well as the methods of development of IT services and methods of prevention of intellectual diversions and violation of information confidentiality. It is offered to implement the program of equal initial possibilities for intellectual development of human resources in view of access to higher education, creative activities, as well as legal protection for everyone, etc. Analysis of implementation of innovational methods of intellectual labor development supposes planning activities in view of development of intellectual labor for the purpose of the region’s economy’s security.peer-reviewe

Манипуляция как форма проявления насилия В семье: эмпирический подход к рассмотрению проблемы

The article reflects the results of an empirical understanding of the problem of the manipulative interaction of children in relation to their parents. Based on the results of using the method of theoretical analysis and appealing to the works of both foreign - E. Bern, F. Zimbardo, R. Krauta, D. Price, M. Smith, R. Cialdini, E. Shostroma, and domestic – Afanasieva V.A., Bravoslavska S.V., Dotsenko E.L., Kara-Murza S., Kozachek O.V., Orlova L.V., Chesnokova O.B., Scherban E. and many other scientists, we believe that manipulation as a behavioral manifestation of people towards each other, can be considered not only as a form of influence and pressure, but also as a manifestation of violence. Of particular relevance are issues related to the manipulative interaction of parents in relation to their children and children in relation to their parents (the work of Bern E., Zimbardo F., Kraut R., Price D., Smith M., Shostroma E., Caldini R., etc.); Afanasieva V.A., Bravoslavska S.V., Dotsenko E.L., Kozachek O.V., Kara-Murza S., Orlova L.V., Chesnokova OB, Scherban E., etc.). Based on our analysis of research in the field of interest, we formulate the assumption that children manipulators grow in those families in which parents actively use this form of influence on others. In order to confirm or refute this assumption, we have developed a monitoring program, the implementation of which suggests that our hypothesis is confirmed. It is in those families that use ineffective contacts and forms of interaction with their children (and manipulation, we refer to this category of contacts), children grow up as manipulators, actively applying this form of violent influence on their parents.El artículo refleja los resultados de una comprensión empírica del problema de la interacción manipuladora de los niños en relación con sus padres. Basado en los resultados del uso del método de análisis teórico y atractivo para los trabajos de ambos extranjeros: E. Bern, F. Zimbardo, R. Krauta, D. Price, M. Smith, R. Cialdini, E. Shostroma y domésticos. - Afanasieva VA, Bravoslavska SV, Dotsenko EL, Kara-Murza S., Kozachek OV, Orlova LV, Chesnokova OB, Scherban E. y muchos otros científicos, creemos que la manipulación como una manifestación conductual de las personas entre sí, puede considerarse no solo como una forma de influencia y presión, sino también como una manifestación de violencia. De particular relevancia son los temas relacionados con la interacción manipuladora de los padres en relación con sus hijos y los niños en relación con sus padres (el trabajo de Bern E., Zimbardo F., Kraut R., Price D., Smith M., Shostroma E ., Caldini R., etc.); Afanasieva V.A., Bravoslavska S.V., Dotsenko E.L., Kozachek O.V., Kara-Murza S., Orlova L.V., Chesnokova OB, Scherban E., etc.). Con base en nuestro análisis de investigación en el campo de interés, formulamos la suposición de que los manipuladores de niños crecen en aquellas familias en las que los padres usan activamente esta forma de influencia en los demás. Para confirmar o refutar esta suposición, hemos desarrollado un programa de monitoreo, cuya implementación sugiere que nuestra hipótesis está confirmada. Es en esas familias que usan contactos ineficaces y formas de interacción con sus hijos (y manipulación, nos referimos a esta categoría de contactos), los niños crecen como manipuladores, aplicando activamente esta forma de influencia violenta en sus padres.В статье отражены результаты эмпирического осмысления проблемы манипулятивного взаимодействия детей по отношению к своим родителям. Основываясь на результатах использования метода теоретического анализа и апеллируя к работам как зарубежных-Э. Берн, Ф. Зимбардо, Р. краута, Д. Прайс, М. Смит, Р. Чалдини, Е. Шостром, так и отечественных-Афанасьева В. А., Бравославская С. В., Доценко Е. Л., Кара-Мурза С., Козачек О. В., Орлова Л. В., Чеснокова О. Б., Щербань Е. и многие другие ученые, мы считаем, что манипуляция как поведенческое проявление людей по отношению друг к другу, может рассматриваться не только как форма влияния и давления, но и как проявление насилия. Особую актуальность приобретают вопросы, связанные с манипулятивным взаимодействием родителей по отношению к своим детям и детей по отношению к своим родителям (работы Берна Э., Зимбардо Ф., краута Р., Прайса Д., Смита М., Шострома Е., Кальдини р. и др.); Афанасьева В. А., Бравославская С. В., Доценко Е. Л., Козачек О. В., Кара-Мурза С., Орлова Л. В., Чеснокова О. Б., Щербань Е. и др.). На основе проведенного нами анализа исследований в интересующей нас области сформулировано предположение о том, что дети-манипуляторы растут в тех семьях, в которых родители активно используют эту форму воздействия на окружающих. Для того, чтобы подтвердить или опровергнуть это предположение, мы разработали программу мониторинга, реализация которого предполагает, что наша гипотеза подтверждается. Именно в тех семьях, которые используют неэффективные контакты и формы взаимодействия со своими детьми (а манипуляцию мы относим к этой категории контактов), дети растут как манипуляторы, активно применяя эту форму насильственного воздействия на своих родителей

Derivatives of 9-phosphorylated acridine as butyrylcholinesterase inhibitors with antioxidant activity and the ability to inhibit β-amyloid self-aggregation: potential therapeutic agents for Alzheimer’s disease

We investigated the inhibitory activities of novel 9-phosphoryl-9,10-dihydroacridines and 9-phosphorylacridines against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and carboxylesterase (CES). We also studied the abilities of the new compounds to interfere with the self-aggregation of β-amyloid (Aβ42) in the thioflavin test as well as their antioxidant activities in the ABTS and FRAP assays. We used molecular docking, molecular dynamics simulations, and quantum-chemical calculations to explain experimental results. All new compounds weakly inhibited AChE and off-target CES. Dihydroacridines with aryl substituents in the phosphoryl moiety inhibited BChE; the most active were the dibenzyloxy derivative 1d and its diphenethyl bioisostere 1e (IC50 = 2.90 ± 0.23 µM and 3.22 ± 0.25 µM, respectively). Only one acridine, 2d, an analog of dihydroacridine, 1d, was an effective BChE inhibitor (IC50 = 6.90 ± 0.55 μM), consistent with docking results. Dihydroacridines inhibited Aβ42 self-aggregation; 1d and 1e were the most active (58.9% ± 4.7% and 46.9% ± 4.2%, respectively). All dihydroacridines 1 demonstrated high ABTS•+-scavenging and iron-reducing activities comparable to Trolox, but acridines 2 were almost inactive. Observed features were well explained by quantum-chemical calculations. ADMET parameters calculated for all compounds predicted favorable intestinal absorption, good blood–brain barrier permeability, and low cardiac toxicity. Overall, the best results were obtained for two dihydroacridine derivatives 1d and 1e with dibenzyloxy and diphenethyl substituents in the phosphoryl moiety. These compounds displayed high inhibition of BChE activity and Aβ42 self-aggregation, high antioxidant activity, and favorable predicted ADMET profiles. Therefore, we consider 1d and 1e as lead compounds for further in-depth studies as potential anti-AD preparations

Isolation and Characterization of a Novel Hydrophobin, Sa-HFB1, with Antifungal Activity from an Alkaliphilic Fungus, Sodiomyces alkalinus

The adaptations that alkaliphilic microorganisms have developed due to their extreme habitats promote the production of active natural compounds with the potential to control microorganisms, causing infections associated with healthcare. The primary purpose of this study was to isolate and identify a hydrophobin, Sa-HFB1, from an alkaliphilic fungus, Sodiomyces alkalinus. A potential antifungal effect against pathogenic and opportunistic fungi strains was determined. The MICs of Sa-HFB1 against opportunistic and clinical fungi ranged from 1 to 8 µg/mL and confirmed its higher activity against both non- and clinical isolates. The highest level of antifungal activity (MIC 1 µg/mL) was demonstrated for the clinical isolate Cryptococcus neoformans 297 m. The hydrophobin Sa-HFB1 may be partly responsible for the reported antifungal activity of S. alkalinus, and may serve as a potential source of lead compounds, meaning that it can be developed as an antifungal drug candidate

Recombinant Cathepsin L of Tribolium castaneum and Its Potential in the Hydrolysis of Immunogenic Gliadin Peptides

Wheat gliadins contain a large amount of glutamine- and proline-rich peptides which are not hydrolyzed by human digestive peptidases and can cause autoimmune celiac disease and other forms of gluten intolerance in predisposed people. Peptidases that efficiently cleave such immunogenic peptides can be used in enzyme therapy. The stored product insect pest Tribolium castaneum efficiently hydrolyzes gliadins. The main digestive peptidase of T. castaneum is cathepsin L, which is from the papain C1 family with post-glutamine cleavage activity. We describe the isolation and characterization of T. castaneum recombinant procathepsin L (rpTcCathL1, NP_001164001), which was expressed in Pichia pastoris cells. The activation of the proenzyme was conducted by autocatalytic processing. The effects of pH and proenzyme concentration in the reaction mixture on the processing were studied. The mature enzyme retained high activity in the pH range from 5.0 to 9.0 and displayed high pH-stability from 4.0 to 8.0 at 20 °C. The enzyme was characterized according to electrophoretic mobility under native conditions, activity and stability at various pH values, a sensitivity to various inhibitors, and substrate specificity, and its hydrolytic effect on 8-, 10-, 26-, and 33-mer immunogenic gliadins peptides was demonstrated. Our results show that rTcCathL1 is an effective peptidase that can be used to develop a drug for the enzyme therapy of various types of gluten intolerance

Conjugates of Tacrine with Salicylamide as Promising Multitarget Agents for Alzheimer’s Disease

New conjugates of tacrine and salicylamide with alkylene spacers were synthesized and evaluated as potential multifunctional agents for Alzheimer’s disease (AD). The compounds exhibited high acetylcholinesterase (AChE, IC50 to 0.224 μM) and butyrylcholinesterase (BChE, IC50 to 0.0104 μM) inhibitory activities. They were also rather poor inhibitors of carboxylesterase, suggesting a low tendency to exert potential unwanted drug-drug interactions in clinical use. The conjugates were mixed-type reversible inhibitors of both cholinesterases and demonstrated dual binding to the catalytic and peripheral anionic sites of AChE in molecular docking that, along with experimental results on propidium iodide displacement, suggest their potential to block AChE-induced β-amyloid aggregation. The new conjugates exhibited high ABTS.+-scavenging activity. N-(6-(1,2,3,4-Tetrahydroacridin-9-ylamino)hexyl)salicylamide is a lead compound that also demonstrates metal chelating ability toward Cu2+, Fe2+ and Zn2+. Thus, the new conjugates have displayed the potential to be multifunctional anti-AD agents for further development.Conjugates of tacrine and salicylamide were synthesized as potential multifunctional anti-AD agents. The compounds have high anticholinesterase (IC50 AChE to 0.22 μM, IC50 BChE to 0.01 μM) and low anti-CES activity, displaced propidium iodide from the AChE PAS, being in agreement with the results of molecular docking. The conjugates exhibited ABTS+-scavenging (TEAC to 0.9) and metal-chelating activity.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/172840/1/cmdc202200080_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/172840/2/cmdc202200080.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/172840/3/cmdc202200080-sup-0001-misc_information.pd

Exploring Peptaibol’s Profile, Antifungal, and Antitumor Activity of Emericellipsin A of <i>Emericellopsis</i> Species from Soda and Saline Soils

Features of the biochemical adaptations of alkaliphilic fungi to exist in extreme environments could promote the production of active antibiotic compounds with the potential to control microorganisms, causing infections associated with health care. Thirty-eight alkaliphilic and alkalitolerant Emericellopsis strains (E. alkalina, E. cf. maritima, E. cf. terricola, Emericellopsis sp.) isolated from different saline soda soils and belonging to marine, terrestrial, and soda soil ecological clades were investigated for emericellipsin A (EmiA) biosynthesis, an antifungal peptaibol previously described for Emericellopsis alkalina. The analysis of the Emericellopsis sp. strains belonging to marine and terrestrial clades from chloride soils revealed another novel form with a mass of 1032.7 Da, defined by MALDI-TOF Ms/Ms spectrometers, as the EmiA lacked a hydroxyl (dEmiA). EmiA displayed strong inhibitory effects on cell proliferation and viability of HCT 116 cells in a dose- and time-dependent manners and induced apoptosis