Selective nanosensor based on folic acid imprinted nanostructures

Folic acid, which provides the transfer of single carbon atoms in synthesis reactions and metabolic cycles in metabolism, is very important for metabolism. Folic acid also plays an important role in nucleotide synthesis and methylation reactions. There are many disorders caused by defective folic acid metabolism and lack of folic acid. Today, innovative, cost-effective methods are needed to develop folic acid determination methods. The main objective of this study is the development of surface-printed carbon electrodes (SPOE) modified with folic acid imprinted nanostructures (FA-Imp-poly(MPTS-rGO-co-NAT), which will be used for the first time for folic acid determination in commercially human blood serum. For this purpose, the synthesis of nanostructures has been carried out and characterized by FTIR, SEM-EDS, and AFM. Then, a new chemically modified nanosensor was fabricated for the determination of folic acid using folic acid imprinted nanostructures. Differential pulse voltammetry (DPV) and circular voltammetry (CV) methods were used as electrochemical methods in the FA-imprinted-nanosensor studies. Measurements in differential pulse voltammetry were performed at an application speed of 0.005 volts per second in the potential range of -0.4 to 0.6 volts. As a result of the circular voltammetric method, an idea about the surface was obtained with the voltammograms obtained. The detection limit (LOD) of the developed FA-imprinted-nanosensor was 7.54 ng/mL and the determination limit (LOQ) was 25.14 ng/mL. FA analytical (10 and 20 ng/mL) was added to commercial synthetic serum samples by the standard adding method and RSD values of 0.092% and 0.734% were found in the DPV technique and measurements respectively. This manuscript demonstrated a novel, simple, selective, and rapid FA-imprinted-nanosensor for determining the FA in the biological samples.This study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK-219S055). The authors would like to thank Canser Kusat Lanpir and Melis Ol for drawing the images in the manuscript.Scientific and Technological Research Council of Turkey [TUBITAK-219S055

Pathway-Centric Analysis of the TCGA - NSCLC Transcriptome Data Pertaining to Deceased Patients

Lung cancer among other cancer types is the most prevalent disease with about 1.9 million new cases observed each year and ranks the fifth most common cause of death according to the World Health Organization. Of the two main subtypes of lung cancer, non-small cell lung carcinoma (NSCLC) accounts for the majority of all cases. The two major subtypes of NSCLC, lung squamous cell carcinoma and lung adenocarcinoma are extensively sampled as part of The Cancer Genome Atlas (TCGA) project. In this study, we took a pathway centric focus on the analysis of RNA-Seq data belonging to dead NSCLC patients involved in TCGA. We applied statistical tests comparing subgroups of patients based on varying clinical traits such as tumor pathologic staging and the total number of days passed from diagnosis till death for the subjected patients. We also compared the transcriptome of smokers and nonsmokers to gain insights about the effect of tobacco smoking history on the gene expression patterns of deceased NSCLC patients. Neuroactive ligand-receptor interaction pathway was overrepresented in patients who died at pathologic T2 stage as well as in those lung cancer patients who survived less than a year. Down-regulation of the signaling pathways such as retrograde endocannabinoid, Hippo, AGE-RAGE in diabetic complications, Wnt, and oxytocin was also striking in patients who survived shorter. We show concordant results with previous findings about the down-regulation of nitric oxide synthase in smokers. This study may encourage more focused analyses of the TCGA data with a potential to cast new perspectives onto lung cancer research

On‐Site Testosterone Biosensing for Doping Detection: Electrochemical Immunosensing via Functionalized Magnetic Nanoparticles and Screen‐Printed Electrodes

Point-of-care approaches are in constant need of novel and highly performant portable devices. Doping is a constant issue seen in the athletic field, and a need for mobile and trustable methods for detection are highly sought. The current work proposes a portable biosensor based on a screen-printed electrode (SPE) and functionalized magnetic particles for testosterone detection. The functionalized magnetic particles were characterized with SEM and the designed biosensor was analyzed amperometrically (Pulse Voltammetry (DPV), Cyclic Voltammetry (CV), and Electrochemical Impedance Spectrometry (EIS)). Results demonstrate linearity between 50-1000 ng/mL with a LOD of 23.68 ng/mL. Q-TOF/MS method was performed to compare with the biosensor over serum and urine. Furthermore, the selectivity of the biosensor was tested in the presence of various interferents. The designed biosensor shows good potential in doping detection and prevention of illicit use due to the versatility of application that it covers.Ege University Research FoundationEge University [18-EGEMATAL-001]Ege University Research Foundation is acknowledged for the financial support (18-EGEMATAL-001). SEM and chromatographic analyses were performed at EGE MATAL (Ege University/Izmir)

A biosensor platform based on amine functionalized conjugated benzenediamine-benzodithiophene polymer for testosterone analysis

Bulut, Umut/0000-0002-4282-5233; CEVHER, SEVKI CAN/0000-0003-2087-2802; SANLI, Serdar/0000-0002-9048-287XWOS: 000528338100001A novel benzenediamine-benzodithiophene polymer is synthesized for use in biosensor fabrication for the detection of testosterone. the sensory platform is constructed via drop coating on a screen-printed carbon electrode, using poly(benzenediamine-Bis[(2-ethylhexyl)oxy]benzodithiophene) (pBDBT) as the polymer layer. Testosterone antibodies are immobilized on the polymer-coated electrode surface via glutaraldehyde, which binds to the surface through the amino functional groups on the polymer backbone. the changes in the surface features due to testosterone binding are investigated via electrochemical techniques such as differential pulse voltammetry, cyclic voltammetry, and electrochemical impedance spectrometry as well as contact angle measurements. Surface morphology of the modified electrodes is characterized by atomic force microscopy. the linear range and limit of detection of the sensor are calculated. Impact of possible interfering compounds is investigated. Furthermore, the sensory platform is utilized for testosterone analysis in synthetic biological fluids.Ege Universitesi [18-EGEMATAL-001]Ege Universitesi, Grant/Award Number: 18-EGEMATAL-00

A biosensor platform based on amine functionalized conjugated benzenediamine‐benzodithiophene polymer for testosterone analysis

A novel benzenediamine-benzodithiophene polymer is synthesized for use in biosensor fabrication for the detection of testosterone. the sensory platform is constructed via drop coating on a screen-printed carbon electrode, using poly(benzenediamine-Bis[(2-ethylhexyl)oxy]benzodithiophene) (pBDBT) as the polymer layer. Testosterone antibodies are immobilized on the polymer-coated electrode surface via glutaraldehyde, which binds to the surface through the amino functional groups on the polymer backbone. the changes in the surface features due to testosterone binding are investigated via electrochemical techniques such as differential pulse voltammetry, cyclic voltammetry, and electrochemical impedance spectrometry as well as contact angle measurements. Surface morphology of the modified electrodes is characterized by atomic force microscopy. the linear range and limit of detection of the sensor are calculated. Impact of possible interfering compounds is investigated. Furthermore, the sensory platform is utilized for testosterone analysis in synthetic biological fluids.Ege Universitesi [18-EGEMATAL-001]Ege Universitesi, Grant/Award Number: 18-EGEMATAL-00

Evaluation of pulmonary vascular resistance and vasoreactivity testing with oxygen in children with congenital heart disease and pulmonary arterial hypertension

cevik, berna saylan/0000-0002-5384-4982WOS: 000338107100021PubMed: 24566516