6 research outputs found
Melting Domain Size and Recrystallization Dynamics of Ice Revealed by Time-Resolved X-ray Scattering
The phase transition between water and ice is ubiquitous and one of the most
important phenomena in nature. Here, we performed time-resolved x-ray
scattering experiments capturing the melting and recrystallization dynamics of
ice. The ultrafast heating of ice I is induced by an IR laser pulse and probed
with an intense x-ray pulse, which provided us with direct structural
information on different length scales. From the wide-angle x-ray scattering
(WAXS) patterns, the molten fraction, as well as the corresponding temperature
at each delay, were determined. The small-angle x-ray scattering (SAXS)
patterns, together with the information extracted from the WAXS analysis,
provided the time-dependent change of the size and the number of the liquid
domains. The results show partial melting (~13 %) and superheating of ice
occurring at around 20 ns. After 100 ns, the average size of the liquid domains
grows from about 2.5 nm to 4.5 nm by the coalescence of approximately six
adjacent domains. Subsequently, we capture the recrystallization of the liquid
domains, which occurs on microsecond timescales due to the cooling by heat
dissipation and results to a decrease of the average liquid domain size
Improved Parameterization of Protein-DNA Interactions for Molecular Dynamics Simulations of PCNA Diffusion on DNA
© 2020 American Chemical Society, As the field of molecular dynamics simulation utilizing the force fields is moving toward more complex systems, the accuracy of intermolecular interactions has become a central issue of the field. Here, we quantitatively evaluate the accuracy of the protein-DNA interactions in AMBER and CHARMM force fields by comparing experimental and simulated diffusion coefficients of proliferating cell nuclear antigen. We find that both force fields underestimate diffusion coefficients by at least an order of magnitude because the interactions between basic amino acids and DNA phosphate groups are too attractive. Then, we propose Lennard-Jones parameters optimized using the experimental osmotic pressure data of model chemicals, by using which one can reproduce the experimental diffusion coefficients. Newly optimized parameters will have a broad impact on general protein-DNA interactions11Nsciescopu
Liquid-liquid phase separation in supercooled water from ultrafast heating of low-density amorphous ice
Recent experiments continue to find evidence for a liquid-liquid phase transition (LLPT) in supercooled water, which would unify our understanding of the anomalous properties of liquid water and amorphous ice. These experiments are challenging because the proposed LLPT occurs under extreme metastable conditions where the liquid freezes to a crystal on a very short time scale. Here, we analyze models for the LLPT to show that coexistence of distinct high-density and low-density liquid phases may be observed by subjecting low-density amorphous (LDA) ice to ultrafast heating. We then describe experiments in which we heat LDA ice to near the predicted critical point of the LLPT by an ultrafast infrared laser pulse, following which we measure the structure factor using femtosecond x-ray laser pulses. Consistent with our predictions, we observe a LLPT occurring on a time scale 1 μs.11Ysciescopu
Experimental observation of the liquid-liquid transition in bulk supercooled water under pressure
We prepared bulk samples of supercooled liquid water under pressure by isochoric heating of high-density amorphous ice to temperatures of 205 +/- 10 kelvin, using an infrared femtosecond laser. Because the sample density is preserved during the ultrafast heating, we could estimate an initial internal pressure of 2.5 to 3.5 kilobar in the high-density liquid phase. After heating, the sample expanded rapidly, and we captured the resulting decompression process with femtosecond x-ray laser pulses at different pump-probe delay times. A discontinuous structural change occurred in which low-density liquid domains appeared and grew on time scales between 20 nanoseconds to 3 microseconds, whereas crystallization occurs on time scales of 3 to 50 microseconds. The dynamics of the two processes being separated by more than one order of magnitude provides support for a liquid-liquid transition in bulk supercooled water.11Nsciescopu
Experimental observation of the liquid-liquid transition in bulk supercooled water under pressure
We prepared bulk samples of supercooled liquid water under pressure by isochoric heating of high-density amorphous ice to temperatures of 205 ± 10 kelvin, using an infrared femtosecond laser. Because the sample density is preserved during the ultrafast heating, we could estimate an initial internal pressure of 2.5 to 3.5 kilobar in the high-density liquid phase. After heating, the sample expanded rapidly, and we captured the resulting decompression process with femtosecond x-ray laser pulses at different pump-probe delay times. A discontinuous structural change occurred in which low-density liquid domains appeared and grew on time scales between 20 nanoseconds to 3 microseconds, whereas crystallization occurs on time scales of 3 to 50 microseconds. The dynamics of the two processes being separated by more than one order of magnitude provides support for a liquid-liquid transition in bulk supercooled water