7 research outputs found
Religion and the Postmodern in Contemporary North American Fiction
Broadly speaking, postmodernist thinking presumes the failure of traditional value systems and epistemologies, reacting to this crisis in truth and knowledge with radical skepticism. This perspective privileges relativity over objective truth, interpretation over meaning, an infinity of multiple perspectives over unified systems of thought and belief, and concrete principles over abstract expressions. As Western religion insists upon exclusive universalized truths and principles, the persistence and even resurgence of religion and religious fundamentalism in a contemporary historical moment otherwise characterized by the pervasive influence of postmodernist tenets in secular life presents a striking paradox. The novels examined in this thesis all variously attempt to explain this apparent contradiction – how postmodern society seems to reject totalizing systems of knowledge and value, but encourages religion and its universalizing conceptions. In different ways, these novels frame religion as a pragmatic reaction to societal anxieties, rather than the result of divine revelation, emphasizing how beliefs morph in response to societal crises. They critique the concept of the religious grand narrative, demonstrating its susceptibility to change and its inability to provide a full story. Finally, these texts address what happens when traditional religious beliefs fail according to postmodern logic, and suggest that people engage the secular to replace the system of belief religion once provided. These novels suggest a human tendency to yearn for systems of belief but simultaneously deny any credibility to an overarching narrative, affirming postmodern society’s attraction to multiplicity while still perhaps allowing for the human need for systems of knowledge and value
Annotating Transcriptional Effects of Genetic Variants in Disease-Relevant Tissue: Transcriptome-Wide Allelic Imbalance in Osteoarthritic Cartilage
Objective. Multiple single-nucleotide polymorphisms (SNPs) conferring susceptibility to osteoarthritis (OA) mark
imbalanced expression of positional genes in articular cartilage, reflected by unequally expressed alleles among heterozygotes (allelic imbalance [AI]). We undertook this study to explore the articular cartilage transcriptome from OA
patients for AI events to identify putative disease-driving genetic variation.
Methods. AI was assessed in 42 preserved and 5 lesioned OA cartilage samples (from the Research Arthritis and
Articular Cartilage study) for which RNA sequencing data were available. The count fraction of the alternative alleles
among the alternative and reference alleles together (φ) was determined for heterozygous individuals. A meta-analysis
was performed to generate a meta-φ and P value for each SNP with a false discovery rate (FDR) correction for multiple comparisons. To further validate AI events, we explored them as a function of multiple additional OA features.
Results. We observed a total of 2,070 SNPs that consistently marked AI of 1,031 unique genes in articular cartilage. Of these genes, 49 were found to be significantly differentially expressed (fold change 2, FDR <0.05)
between preserved and paired lesioned cartilage, and 18 had previously been reported to confer susceptibility to
OA and/or related phenotypes. Moreover, we identified notable highly significant AI SNPs in the CRLF1, WWP2, and
RPS3 genes that were related to multiple OA features.
Conclusion. We present a framework and resulting data set for researchers in the OA research field to probe for
disease-relevant genetic variation that affects gene expression in pivotal disease-affected tissue. This likely includes
putative novel compelling OA risk genes such as CRLF1, WWP2, and RPS3
Annotating Transcriptional Effects of Genetic Variants in Disease-Relevant Tissue: Transcriptome-Wide Allelic Imbalance in Osteoarthritic Cartilage
OBJECTIVE: Multiple single-nucleotide polymorphisms (SNPs) conferring susceptibility to osteoarthritis (OA) mark imbalanced expression of positional genes in articular cartilage, reflected by unequally expressed alleles among heterozygotes (allelic imbalance [AI]). We undertook this study to explore the articular cartilage transcriptome from OA patients for AI events to identify putative disease-driving genetic variation. METHODS: AI was assessed in 42 preserved and 5 lesioned OA cartilage samples (from the Research Arthritis and Articular Cartilage study) for which RNA sequencing data were available. The count fraction of the alternative alleles among the alternative and reference alleles together (phi) was determined for heterozygous individuals. A meta-analysis was performed to generate a meta-phi and P value for each SNP with a false discovery rate (FDR) correction for multiple comparisons. To further validate AI events, we explored them as a function of multiple additional OA features. RESULTS: We observed a total of 2,070 SNPs that consistently marked AI of 1,031 unique genes in articular cartilage. Of these genes, 49 were found to be significantly differentially expressed (fold change 2, FDR <0.05) between preserved and paired lesioned cartilage, and 18 had previously been reported to confer susceptibility to OA and/or related phenotypes. Moreover, we identified notable highly significant AI SNPs in the CRLF1, WWP2, and RPS3 genes that were related to multiple OA features. CONCLUSION: We present a framework and resulting data set for researchers in the OA research field to probe for disease-relevant genetic variation that affects gene expression in pivotal disease-affected tissue. This likely includes putative novel compelling OA risk genes such as CRLF1, WWP2, and RPS3
Hyperlipidemia in glycogen storage disease type III: Effect of age and metabolic control
While the presence of hyperlipidaemia in glycogen storage disease (GSD) type Ia and Ib is generally accepted, few investigators have adequately assessed lipid profiles of GSD III in children, in whom the presence of hyperlipidaemia may be most prominent. We analysed the lipid profiles in 44 GSD III patients from 6 months to 30 years of age. Hypertriglyceridaemia and hypercholesterolaemia were common in children younger than 3 years of age. Hypertriglyceridaemia correlated negatively with age, and may reflect increased severity of hypoglycaemia in this younger population. The presence of hyperlipidaemia during childhood in these patients identifies another GSD population that could be at risk for early cardiovascular disease (CVD). Consequently, the outcome of clinical trials investigating the vascular effect of hyperlipidaemia in GSD applies to types other than GSD I
Hyperlipidemia in glycogen storage disease type III: Effect of age and metabolic control
Identification of human glycosyltransferase genes expressed in erythroid cells predicts potential carbohydrate blood group loci
Glycans are biologically important structures synthesised by glycosyltransferase (GT) enzymes. Disruptive genetic null variants in GT genes can lead to serious illness but benign phenotypes are also seen, including antigenic differences on the red blood cell (RBC) surface, giving rise to blood groups. To characterise known and potential carbohydrate blood group antigens without a known underlying gene, we searched public databases for human GT loci and investigated their variation in the 1000 Genomes Project (1000 G). We found 244 GT genes, distributed over 44 families. All but four GT genes had missense variants or other variants predicted to alter the amino acid sequence, and 149 GT genes (61%) had variants expected to cause null alleles, often associated with antigen-negative blood group phenotypes. In RNA-Seq data generated from erythroid cells, 155 GT genes were expressed at a transcript level comparable to, or higher than, known carbohydrate blood group loci. Filtering for GT genes predicted to cause a benign phenotype, a set of 30 genes remained, 16 of which had variants in 1000 G expected to result in null alleles. Our results identify potential blood group loci and could serve as a basis for characterisation of the genetic background underlying carbohydrate RBC antigens