Identification of virulence factors and antibiotic resistance markers using bacterial genomics.

International audienceIn recent years, the number of multidrug-resistant bacteria has increased rapidly and several epidemics were signaled in different regions of the world. Faced with this situation that presents a major global public health concern, the development and the use of new and rapid technologies is more than urgent. The use of the next-generation sequencing platforms by microbiologists and infectious disease specialists has allowed great progress in the medical field. Here, we review the usefulness of whole-genome sequencing for the detection of virulence and antibiotic resistance associated genes

Identification of virulence factors and antibiotic resistance markers using bacterial genomics.

International audienceIn recent years, the number of multidrug-resistant bacteria has increased rapidly and several epidemics were signaled in different regions of the world. Faced with this situation that presents a major global public health concern, the development and the use of new and rapid technologies is more than urgent. The use of the next-generation sequencing platforms by microbiologists and infectious disease specialists has allowed great progress in the medical field. Here, we review the usefulness of whole-genome sequencing for the detection of virulence and antibiotic resistance associated genes

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Not AvailableThe aim of the present study was to characterize ten selected isolates of Trichoderma with differential inhibitory potential over the collar rot pathogen of Amorphophallus, Sclerotium rolfsii. Use of a polyphasic approach which combined the morphological, molecular and biochemical attributes revealed inter specific and intra specific diversity among different isolates. Strains with the best in vitro antagonistic capacity were Trichoderma harzianum (Tr9) and Trichoderma asperellum (Tr10). In general, relationship was observed between the anatgonistic capacity of the Trichoderma spp. and their in vitro biocontrol performance, cell wall degrading enzyme production, protein profiles and the RAPD and AFLP finger print. The pot experiment revealed a comparative success of Tr9 in controlling collar rot incidence in Amorphophallus over Tr10. This is the first report of intensive characterization of Trichoderma strains with the integration of the above data sets against S. rolfsii, in an attempt to explore their biocontrol potential in managing collar rot disease.Not Availabl

High-quality draft genome sequence and description of Haemophilus massiliensis sp nov.

International audienceStrain FF7(T) was isolated from the peritoneal fluid of a 44-year-old woman who suffered from pelvic peritonitis. This strain exhibited a 16S rRNA sequence similarity of 94.8 % 16S rRNA sequence identity with Haemophilus parasuis, the phylogenetically closest species with a name with standing in nomenclature and a poor MALDI-TOF MS score (1.32 to 1.56) that does not allow any reliable identification. Using a polyphasic study made of phenotypic and genomic analyses, strain FF7(T) was a Gram-negative, facultatively anaerobic rod and member of the family Pasteurellaceae. It exhibited a genome of 2,442,548 bp long genome (one chromosome but no plasmid) contains 2,319 protein-coding and 67 RNA genes, including 6 rRNA operons. On the basis of these data, we propose the creation of Haemophilus massiliensis sp. nov. with strain FF7(T) (= CSUR P859 = DSM 28247) as the type strain