Quantifying heterogeneity according to deformation of the U937 monocytes and U937-differentiated macrophages using 3D carbon dielectrophoresis in microfluidics

A variety of force fields have thus far been demonstrated to investigate electromechanical properties of cells in a microfluidic platform which, however, are mostly based on fluid shear stress and may potentially cause irreversible cell damage. This work presents dielectric movement and deformation measurements of U937 monocytes and U937-differentiated macrophages in a low conductive medium inside a 3D carbon electrode array. Here, monocytes exhibited a crossover frequency around 150 kHz and presented maximum deformation index at 400 kHz and minimum deformation index at 1 MHz frequencies at 20 Vpeak-peak. Although macrophages were differentiated from monocytes, their crossover frequency was lower than 50 kHz at 10 Vpeak-peak. The change of the deformation index for macrophages was more constant and lower than the monocyte cells. Both dielectric mobility and deformation spectra revealed significant differences between the dielectric responses of U937 monocytes and U937-differentiated macrophages, which share the same origin. This method can be used for label-free, specific, and sensitive single-cell characterization. Besides, damage of the cells by aggressive shear forces can, hence, be eliminated and cells can be used for downstream analysis. Our results showed that dielectric mobility and deformation have a great potential as an electromechanical biomarker to reliably characterize and distinguish differentiated cell populations from their progenitors

Single cell level dielectrophoretic responses & dielectrophoretic deformations of monocytes to quantify population heterogeneity

Single-cell dielectrophoretic movement and dielectrophoretic deformation of monocyte cells were interrogated applying 20 V pp , 50 kHz to 1 MHz signal in the 3D carbon electrode array. Heterogeneity of the monocyte population is shown in terms of the crossover frequencies, translational movement, and deformation index of the cells. The results presented that crossover range for monocytes was 100 kHz - 200 kHz, the translational movement of the cells was rapidly altered when the initial positions of the cells were in the negative dielectrophoretic region. Finally, the deformation index of the monocyte population varied from 0.5 to 1.5

Jacob Augustus Lockhart Clarke (1817-1880)

[No Abstract Available]Council of Higher Education (CoHE) of TurkeyThe authors would like to thank the Council of Higher Education (CoHE) of Turkey for funding Esra Candar under 100/2000 CoHE Ph.D. Scholarship Program in the field of Translational Medicine

Synthesis and application of novel magnetite nanoparticle based azacrown ether for protein recognition

WOS: 000319772100012This article is the first report showing the human serum albumin (HSA) binding studies by using a new magnetite nanoparticle containing azacrown ether moieties via solid-liquid extraction process. The structure of the newly prepared magnetite nanoparticle was clarified by using attenuated total reflectance (ATR) infrared spectroscopy, scanning electron microscopy (SEM), UV-vis and elemental analysis. Analytic results indicated that modification of the surface of the magnetite nanoparticles with azacrown ether derivative was successfully carried out. The protein binding studies exhibited that the modified magnetite nanoparticles could be efficiently used for the binding of the human serum albumin (HSA) in aqueous solutions via non-covalent interaction between crown ether cavity and amino group of the protein

Reel 1

Crystal (Drumwright, George); Meditation #3 (Heffner, Michael); Prelude (Blumenthal, Paul); By the Waters (Helms, Art); Prelude (Johnson, George); Trio 1 for flute, cello, and piano (Nahay, Paul); Prelude (Bennett, Brian); Full Moon (Berman, Lewis); Moon and Stars (Berman, Lewis); Light of Peace (Monroe, Thomas); Spatial Elements (Chaudoir, James). Instrumentation: piccolo; flute; oboe; clarinet; trumpet; trombone; piano; percussion; vibraphone; marimba; soprano; mezzo-soprano; baritone; violin; viola; cell

Synthesis of Novel Symmetrical 1,4-Disubstituted 1,2,3-Bistriazole Derivatives via 'Click Chemistry' and Their Biological Evaluation

Alpay Karaoglu, Sengul/0000-0003-1047-8350WOS: 000380241600121PubMed: 27213320A series of symmetric bis-1,2,3-triazole compounds 2-5(a-f) were synthesized as potential antioxidant agents via click chemistry. Their structures were confirmed by H-1-NMR and C-13-NMR. All of the synthesized compounds were subjected to antioxidant and antimicrobial assays. the antioxidant activity of these compounds (AChE inhibition, DPPH and SOD activities) was evaluated. Compound 2f was found to show the highest AChE inhibition activity of all compounds, while compound 3b showed a strong inhibitory effect on DPPH radical and compound 2a was the most effective of all compounds for SOD activity. All synthesized compounds were found to possess moderate antibacterial activity against the bacteria E. coli and Y. pseudotuberculosis.Karadeniz Technical UniversityKaradeniz Technical UniversityThis was carried out by the research fund of Karadeniz Technical University

Macular choroidal thickness measurements in patients with obstructive sleep apnea syndrome

Uzun, Feyzahan/0000-0002-3050-0714WOS: 000349983900052PubMed: 24962956The purpose of this study is to assess macular choroidal thickness measurements in patients with different severities of obstructive sleep apnea syndrome (OSAS) versus normal controls by using enhanced depth imaging optical coherence tomography (EDI-OCT). This paper is a descriptive study. in this prospective study, the macular area of 74 patients with OSAS and 33 controls were evaluated. All subjects underwent complete ophthalmic examination and macular choroidal thickness (CT) measurements by enhanced depth imaging method of the Spectralis optical coherence tomography system. Choroidal thickness (CT) was measured at the fovea and at 1,000-mu m intervals from the foveal center in both temporal and nasal directions by two masked observers. the mean age was not significantly different between patients with OSAS and controls. Patients were grouped as mild (n = 15), moderate (n = 28), and severe (n = 31) according to apnea-hypopnea index (AHI) scores. the mean subfoveal choroidal thickness (SFCT) was 338.0 +/- 85.2 mu m in the control group versus 351.3 +/- 90, 307.8 +/- 65.5, and 325.4 +/- 110.2 mu m in mild, moderate, and severe groups, respectively (p = 0.416). There was no significant correlation between the severity of OSAS and choroidal thickness. the patients with OSAS seem to protect the choroidal thickness despite hypoxia. the role of OSAS in the pathophysiology of choroidal blood flow and choroidal structure needs further investigation

Cytotoxic Activity-Guided Isolation Studies on Fumana procumbens (Dunal) Gren. \& Godr.

Among the plant secondary metabolites much attention has been given to naturally occurring phenolic compounds as attractive candidates for cancer treatment and prevention. Fumana procumbens (Dunal) Gren. \& Godr (Cistaceae) is a perennial herb, distributed in inner and southwest Anatolia, Turkey. F. procumbens was shown to be rich in phenolic constituents especially flavonoids and biflavonoids which are known for their antioxidant and anticarcinogenic effects. Thus, this study aims to investigate the anticancer activity of F. procumbens extracts, fractions and pure compounds by MTT test against HeLa, MCF-7, and A549 as well as BEAS-2B cell lines for determining selectivity. Bioactivity-guided phytochemical investigation on Fumana procumbens (Dunal) Gren. \& Godr. led to the isolation and identification of three biflavonoids: genkwanol A, dihydrodaphnodorin B and stelleranol. The structures were elucidated by extensive 1D- and 2D-NMR spectroscopic analysis in combination with MS experiments. This is the first report on the isolation of genkwanol A and stelleranol from the genus Fumana and from the family Cistaceae. As a result, no individual cytotoxic activity was obtained for biflavonoid compounds. However, the former fractions FrC and FrD as well as MeOH and n-BuOH extracts exerted moderate and significant cytotoxic activity which suggested a possible synergistic effect