Spatio-temporal variations in mortality causes of two migratory forest raptors in Spain

©2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in European Journal of Wildlife Research. To access the final edited and published work see https://doi.org/10.1007/s10344-015-0981-xAnalysis of the 949 and 434 cases of mortality of Booted Eagle Aquila pennata and Short-toed Snake Eagle Circaetus gallicus, respectively, recorded by wildlife rehabil itation centres in Spain over a 16-year period (1990–2006) shows that power lines (19.5 and 35.2 %, respectively) and killing (32.5 and 22.9 %, respectively) were the main known causes of death. Multinomial regression models were used to analyse geographical and temporal variations in the causes of death. For the Booted Eagle, both factors (zone and year) were statistically significant, while there were only significant tem poral variations for the Short-toed Snake Eagle. In the Booted Eagle, killing occurred more frequently than expected in the east and north of the country compared to the other Spanish regions. Power line casualties were significantly more fre quent in the southern and eastern regions, and less common in the north. In both species, the multinomial models indicate that while the number of cases of killing significantly de creased during the 16 years studied, power line casualties increased. Our study suggests that human-induced mortality continues to be the main factor contributing to mortality for Spanish Booted Eagles and Short-toed Snake Eagles. Since a reduction in the mortality caused by human activities is a priority in the conservation strategies for raptor species, man agement guidelines are discusse

Histological findings after intralesional methotrexate treatment in cutaneous squamous cell carcinoma

Intralesional methotrexate (il-MTX) has been reported as a useful therapy in keratoacanthoma (KA) and cutaneous squamous cell carcinoma (cSCC). However, the data available on the histological changes induced by this therapy are very scarce. We conducted a single center, prospective study that included 65 cases of cSCC treated with il-MTX before surgical treatment. Two histological studies were conducted in all patients: before intralesional treatment and after surgical removal. Lesions were assessed longitudinally both clinically and histologically. 60 patients (92.3%) responded to il-MTX treatment. There were no differences regarding aggressive histological features of the cSCC between responder and non-responder patients. All cases showed a chronic inflammatory infiltrate after il-MTX. Intratumoral necrosis areas were frequently observed. All cases showed local fibrosis with fine thickening of collagen bundles. Il-MTX induces a chronic lymphohistiocytic inflammatory reaction in both clinical responder and nonresponder patients. Tumor involution after il-MTX is followed by a fine fibrosis that explains the great cosmetic results and improves the accuracy of the follow-up

Defining dipeptidyl peptidase-4 inhibitors-related bullous pemphigoid: A single-centre retrospective study

Background: Many studies have corroborated the association of dipeptidyl peptidase4 inhibitors (DPP4i) use with bullous pemphigoid (BP). It has been speculated that this drug-induced variant presents with a different clinical spectrum than conventional BP. Objective: To determine the prevalence of DPP4i-induced cases of BP and to evaluate whether gliptin-related BP has specific clinicopathological and immunological features. Methods: We conducted a retrospective, observational study of BP cases attended at our centre between January 2000 and June 2020. Epidemiological, clinical, histopathological and laboratory data were collected. Results: A total of 257 cases of BP were collected; 51 (24.3%) were on treatment with DPP4i. When analysing DPP4i-induced BP cases, generalized BP was the predominant pattern and scalp/mucosal involvement was found in 13 patients. Gliptin-related BP cases were associated to a decrease in the eosinophilic infiltrate (p = 0.000) and both the detection rate and concentration of anti-BP180 IgG were lower (p = 0.004, p = 0.001, respectively) than non-DPP4i cases. Limitations: Retrospective, single-centre study. Conclusion: Our large DPP4i-induced BP case series has highlighted that DPP4iinduced BP is characterized by generalized lesions and scalp involvement. Lower titres of anti-BP180 antibodies and a decrease in eosinophils infiltrating into the skin may be distinct features of DPP4i-related BP