User Satisfaction on the MySejahtera Application among Postgraduate Students in a University in Selangor

This study adapted the Expectation Confirmation Theory (ECT) and Technology Acceptance Model (TAM) framework of user satisfaction on the MySejahtera application among postgraduate students. This study uses a questionnaire based on a quantitative approach, and the questionnaire was distributed using purposive sampling consisting of 176 postgraduate students in a University in Selangor. The respondent’s gender, age, education, information quality, system quality, service quality, perceived ease of use, perceived usefulness and user satisfaction were investigated. Results showed that system quality, service quality, perceived ease of use and perceived usefulness are influenced by user satisfaction, and the hypotheses are supported. In contrast, information quality is not influenced by user satisfaction and the hypothesis is not supported. Keywords: User Satisfaction, MySejahtera Application, Case study, Information Management eISSN: 2398-4287 © 2022. The Authors. Published for AMER ABRA cE-Bs by E-International Publishing House, Ltd., UK. This is an open-access article under the CC  BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer-review under the responsibility of AMER (Association of Malaysian Environment-Behavior Researchers), ABRA (Association of Behavioral Researchers on Asians), and cE-Bs (Centre for Environment-Behavior Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia

A Preliminary Study of the Influence of Social Media Engagement on Information Seeking Behavior among the Secondary School Students

The aim of conducting this research is to examine the influence of social media engagement on information-seeking behavior. There is lack of concern about generalizability and implementation towards emphasizing the effects of social media engagement on information-seeking behavior in Malaysia. A close-ended questionnaire was distributed to the students to conduct the data collection for this research. The data was collected from students and was analyzed using SPSS version 26. Findings show that two hypotheses were accepted; Novelty and Perceived Usability have a positive and significant relationship between information-seeking behavior, while Focus Attention and aesthetics were found insignificant for information-seeking behavior. Keywords: Social media, information-seeking behavior, Social media engagement,  aesthetic, education eISSN: 2398-4287 © 2022. The Authors. Published for AMER ABRA cE-Bs by E-International Publishing House, Ltd., UK. This is an open-access article under the CC  BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer-review under the responsibility of AMER (Association of Malaysian Environment-Behavior Researchers), ABRA (Association of Behavioral Researchers on Asians), and cE-Bs (Centre for Environment-Behavior Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia

Cabaran-cabaran tentang data bersepadu (data integration) / Mohd Ridwan Seman @ Kamarulzaman and Mohd Rozi Remeli @ Rameli

Data bersepadu (Data Integration) adalah merupakan satu teknologi yang amat penting di dunia atas talian (online world) terutamanya di kalangan organisasi perniagaan yang bergantung kepada ketepatan dan kepantasan data di dalam membuat keputusan strategik. Walaubagaimana pun, terdapat pelbagai cabaran dan isu di dalam mengimplementasikan teknologi ini. Antaranya ialah; isu data itu sendiri, isu keselamatan data, isu manusia (pengguna dan penxedia teknologi), teknologi dan perkasasan dan isu kos

A Review on Digestive System of <i>Rhynchophorus ferrugineus</i> as Potential Target to Develop Control Strategies

Rhynchophorus ferrugineus, commonly known as red palm weevil (RPW), is a high-risk insect pest that has become a threat to many important palm species. There are several dominant factors that lead to the successful infestation of RPW, including its stealthy lifestyle, highly chitinized mouthpart, and high fecundity rate. Due to that, millions of dollars of losses have been suffered by many countries invaded by RPW. Several methods have been designed to control its invasion, including the usage of insecticides, but many cause resistance and environmental pollution. Therefore, an environmentally friendly insecticide that targets specific systems or pathways in RPW is urgently needed. One of the potential targets is the digestive system of RPW, as it is the major interface between the insect and its plant host. The related knowledge of RPW’s digestive system, such as the anatomy, microflora, transcriptomic analysis, and proteomic analysis, is important to understand its effects on RPW’s survival. Several data from different omics regarding the digestive systems of RPW have been published in separate reports. Some of the potential targets have been reported to be inhibited by certain potential insecticides, while other targets have not yet been tested with any inhibitors. Hence, this review may lead to a better understanding on managing infestations of RPW using the system biology approach for its digestive system

Novel NAD⁺-Farnesal Dehydrogenase from <i>Polygonum minus</i> Leaves. Purification and Characterization of Enzyme in Juvenile Hormone III Biosynthetic Pathway in Plant

<div><p>Juvenile Hormone III is of great concern due to negative effects on major developmental and reproductive maturation in insect pests. Thus, the elucidation of enzymes involved JH III biosynthetic pathway has become increasing important in recent years. One of the enzymes in the JH III biosynthetic pathway that remains to be isolated and characterized is farnesal dehydrogenase, an enzyme responsible to catalyze the oxidation of farnesal into farnesoic acid. A novel NAD⁺-farnesal dehydrogenase of <i>Polygonum minus</i> was purified (315-fold) to apparent homogeneity in five chromatographic steps. The purification procedures included Gigacap S-Toyopearl 650M, Gigacap Q-Toyopearl 650M, and AF-Blue Toyopearl 650ML, followed by TSK Gel G3000SW chromatographies. The enzyme, with isoelectric point of 6.6 is a monomeric enzyme with a molecular mass of 70 kDa. The enzyme was relatively active at 40°C, but was rapidly inactivated above 45°C. The optimal temperature and pH of the enzyme were found to be 35°C and 9.5, respectively. The enzyme activity was inhibited by sulfhydryl agent, chelating agent, and metal ion. The enzyme was highly specific for farnesal and NAD⁺. Other terpene aldehydes such as <i>trans</i>- cinnamaldehyde, citral and <i>α</i>- methyl cinnamaldehyde were also oxidized but in lower activity. The <i>K</i>_m values for farnesal, citral, trans- cinnamaldehyde, α- methyl cinnamaldehyde and NAD⁺ were 0.13, 0.69, 0.86, 1.28 and 0.31 mM, respectively. The putative <i>P</i>. <i>minus</i> farnesal dehydrogenase that’s highly specific towards farnesal but not to aliphatic aldehydes substrates suggested that the enzyme is significantly different from other aldehyde dehydrogenases that have been reported. The MALDI-TOF/TOF-MS/MS spectrometry further identified two peptides that share similarity to those of previously reported aldehyde dehydrogenases. In conclusion, the <i>P</i>. <i>minus</i> farnesal dehydrogenase may represent a novel plant farnesal dehydrogenase that exhibits distinctive substrate specificity towards farnesal. Thus, it was suggested that this novel enzyme may be functioning specifically to oxidize farnesal in the later steps of JH III pathway. This report provides a basic understanding for recombinant production of this particular enzyme. Other strategies such as adding His-tag to the protein makes easy the purification of the protein which is completely different to the native protein. Complete sequence, structure and functional analysis of the enzyme will be important for developing insect-resistant crop plants by deployment of transgenic plant.</p></div

Effects of pH on farnesal dehydrogenase activity.

<p>Enzyme activity was assayed under the standard assay conditions, except that the following buffers were used at a final concentration of 100 mM in the incubation mixture: sodium citrate buffers (●), potassium phosphate buffers (<b>×</b>), tris-HCl buffers (Δ), and glycine-NaOH buffers (■). Relative activity values for pH are indicated as mean values (n = 3).</p

Purification summary of farnesal dehydrogenase from <i>P</i>. <i>minus</i> leaves.

<p>Purification summary of farnesal dehydrogenase from <i>P</i>. <i>minus</i> leaves.</p

Identification of tryptic peptides from <i>P</i>. <i>minus</i> farnesal dehydrogenase.

<p>Identification of tryptic peptides from <i>P</i>. <i>minus</i> farnesal dehydrogenase.</p

Proteomic profile of epidermal mucus from Labeo rohita reveals differentially abundant proteins after Aeromonas hydrophila infection

We report the proteomic profile of Epidermal Mucus (EM) from Labeo rohita and identified the differentially abundant proteins (DAPs) against Aeromonas hydrophila infection through label-free liquid chromatography-mass spectrometry (LC-MS/MS). Using discovery-based proteomics, a total of 2039 proteins were quantified in nontreated group and 1,328 proteins in the treated group, of which 114 were identified as DAPs in both the groups. Of the 114 DAPs, 68 proteins were upregulated and 46 proteins were downregulated in the treated group compared to nontreated group. Functional annotations of these DAPs shows their association with metabolism, cellular process, molecular process, cytoskeletal, stress, and particularly immune system. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and Fisher's exact test between the two groups shows that most of the proteins were immune-related, which were significantly associated with the proteasome, phagosome, and Salmonella infection pathways. Overall, this study shows a basic and primary way for further functional research of the involvement of vitellogenin 2, alpha-2-macroglobulin-like protein, toll-like receptors (TLR-13), calpain, keratin-like proteins, and heat shock proteins against bacterial infection. Nonetheless, this first-ever comprehensive report of a proteomic sketch of EM from L. rohita after A. hydrophila infection provides systematic protein information to broadly understand the biological role of fish EM against bacterial infection

Aldehydes substrates used for the substrate specificity of farnesal dehydrogenase from <i>P</i>. <i>minus</i> leaves.

<p>Aldehydes substrates used for the substrate specificity of farnesal dehydrogenase from <i>P</i>. <i>minus</i> leaves.</p