Unveiling a key role of oxaloacetate-glutamate interaction in regulation of respiration and ROS generation in nonsynaptic brain mitochondria using a kinetic model.

Glutamate plays diverse roles in neuronal cells, affecting cell energetics and reactive oxygen species (ROS) generation. These roles are especially vital for neuronal cells, which deal with high amounts of glutamate as a neurotransmitter. Our analysis explored neuronal glutamate implication in cellular energy metabolism and ROS generation, using a kinetic model that simulates electron transport details in respiratory complexes, linked ROS generation and metabolic reactions. The analysis focused on the fact that glutamate attenuates complex II inhibition by oxaloacetate, stimulating the latter's transformation into aspartate. Such a mechanism of complex II activation by glutamate could cause almost complete reduction of ubiquinone and deficiency of oxidized form (Q), which closes the main stream of electron transport and opens a way to massive ROS generating transfer in complex III from semiquinone radicals to molecular oxygen. In this way, under low workload, glutamate triggers the respiratory chain (RC) into a different steady state characterized by high ROS generation rate. The observed stepwise dependence of ROS generation on glutamate concentration experimentally validated this prediction. However, glutamate's attenuation of oxaloacetate's inhibition accelerates electron transport under high workload. Glutamate-oxaloacetate interaction in complex II regulation underlies the observed effects of uncouplers and inhibitors and acceleration of Ca2+ uptake. Thus, this theoretical analysis uncovered the previously unknown roles of oxaloacetate as a regulator of ROS generation and glutamate as a modifier of this regulation. The model predicted that this mechanism of complex II activation by glutamate might be operative in situ and responsible for excitotoxicity. Spatial-time gradients of synthesized hydrogen peroxide concentration, calculated in the reaction-diffusion model with convection under a non-uniform local approximation of nervous tissue, have shown that overproduction of H2O2 in a cell causes excess of its level in neighbor cells

Structure of oxidative and sulphate-clorinating roasting products of nickel converter matte

The structure and phase composition of the nickel converter matte roasting products has been studied in the sulphate-chlorinating process stage. In stages of the converter matte oxidation in the "fluidized bed" and flash smelting remainder roasting together with silvinite the distribution of non-ferrous metals on the phase constituents has been estimated. In the article data from optical spectroscopy, as well as X-ray diffraction, microprobe and chemical analyze are used. As following from the composition of the phases formed during sulphate-chlorinating roasting and thermodynamic modeling data a number of reactions proceeding in the process is presented. © 2013 Allerton Press, Inc

Stochastic current switching in bistable resonant tunneling systems

Current-voltage characteristics of resonant-tunneling structures often exhibit intrinsic bistabilities. In the bistable region of the I-V curve one of the two current states is metastable. The system switches from the metastable state to the stable one at a random moment in time. The mean switching time \tau depends exponentially on the bias measured from the boundary of the bistable region V_{th}. We find full expressions for \tau (including prefactors) as functions of bias, sample geometry, and in-plane conductivity. Our results take universal form upon appropriate renormalization of the threshold voltage V_{th}. We also show that in large samples the switching initiates inside, at the edge, or at a corner of the sample depending on the parameters of the system.Comment: 21 pages, 5 figure

Lifetime of metastable states in resonant tunneling structures

We investigate the transport of electrons through a double-barrier resonant-tunneling structure in the regime where the current-voltage characteristics exhibit bistability. In this regime one of the states is metastable, and the system eventually switches from it to the stable state. We show that the mean switching time grows exponentially as the voltage across the device is tuned from the its boundary value into the bistable region. In samples of small area we find that the logarithm of the lifetime is proportional to the voltage (measured from its boundary value) to the 3/2 power, while in larger samples the logarithm of the lifetime is linearly proportional to the voltage.Comment: REVTeX 4, 5 pages, 3 EPS-figure

Particle decay in false vacuum

We revisit the problem of decay of a metastable vacuum induced by the presence of a particle. For the bosons of the `master field' the problem is solved in any number of dimensions in terms of the spontaneous decay rate of the false vacuum, while for a fermion we find a closed expression for the decay rate in (1+1) dimensions. It is shown that in the (1+1) dimensional case an infrared problem of one-loop correction to the decay rate of a boson is resolved due to a cancellation between soft modes of the field. We also find the boson decay rate in the `sine-Gordon staircase' model in the limits of strong and weak coupling.Comment: 19 pages, 2 figure

One-Loop Corrections to Bubble Nucleation Rate at Finite Temperature

We present an evaluation of the 1-loop prefactor in the lifetime of a metastable state which decays at finite temperature by bubble nucleation. Such a state is considered in one-component phi^4 model in three space dimensions. The calculation serves as a prototype application of a fast numerical method for evaluating the functional determinants that appear in semiclassical approximations.Comment: DO-TH-93/18, 15 pages, 11 Figures available on request, LaTeX, no macros neede

Metastability of (d+n)-dimensional elastic manifolds

We investigate the depinning of a massive elastic manifold with $d$ internal dimensions, embedded in a $(d+n)$-dimensional space, and subject to an isotropic pinning potential $V({\bf u})=V(|{\bf u}|).$ The tunneling process is driven by a small external force ${\bf F}.$ We find the zero temperature and high temperature instantons and show that for the case $1\le d\le 6$ the problem exhibits a sharp transition from quantum to classical behavior: At low temperatures $T<T_{c}$ the Euclidean action is constant up to exponentially small corrections, while for $T> T_{c},$ ${S_{\rm Eucl}(d,T)}/{\hbar} = {U(d)}/{T}.$ The results are universal and do not depend on the detailed shape of the trapping potential $V({\bf u})$. Possible applications of the problem to the depinning of vortices in high-$T_{c}$ superconductors and nucleation in $d$-dimensional phase transitions are discussed. In addition, we determine the high-temperature asymptotics of the preexponential factor for the $(1+1)$-dimensional problem.Comment: RevTeX, 10 pages, 3 figures inserte

A search for two body muon decay signals

Lepton family number violation is tested by searching for $\mu^+\to e^+X^0$ decays among the 5.8$\times 10^8$ positive muon decay events analyzed by the TWIST collaboration. Limits are set on the production of both massless and massive $X^0$ bosons. The large angular acceptance of this experiment allows limits to be placed on anisotropic $\mu^+\to e^+X^0$ decays, which can arise from interactions violating both lepton flavor and parity conservation. Branching ratio limits of order $10^{-5}$ are obtained for bosons with masses of 13 - 80 MeV/c$^2$ and with different decay asymmetries. For bosons with masses less than 13 MeV/c$^{2}$ the asymmetry dependence is much stronger and the 90% limit on the branching ratio varies up to $5.8 \times 10^{-5}$. This is the first study that explicitly evaluates the limits for anisotropic two body muon decays.Comment: 7 pages, 5 figures, 2 tables, accepted by PR

No triangles on the moduli space of maximally supersymmetric gauge theory

Maximally supersymmetric gauge theory in four dimensions has a remarkably simple S-matrix at the origin of its moduli space at both tree and loop level. This leads to the question what, if any, of this structure survives at the complement of this one point. Here this question is studied in detail at one loop for the branch of the moduli space parameterized by a vacuum expectation value for one complex scalar. Motivated by the parallel D-brane picture of spontaneous symmetry breaking a simple relation is demonstrated between the Lagrangian of broken super Yang-Mills theory and that of its higher dimensional unbroken cousin. Using this relation it is proven both through an on- as well as an off-shell method there are no so-called triangle coefficients in the natural basis of one-loop functions at any finite point of the moduli space for the theory under study. The off-shell method yields in addition absence of rational terms in a class of theories on the Coulomb branch which includes the special case of maximal supersymmetry. The results in this article provide direct field theory evidence for a recently proposed exact dual conformal symmetry motivated by the AdS/CFT correspondence.Comment: 39 pages, 4 figure