PHYTOCHEMICAL PROFILING, FREE-RADICAL SCAVENGING AND ANTI-INFLAMMATORY ACTIVITIES OF MELIOSMA SIMPLICIFOLIA (L.)

Objective: In the current research, to determine the stem extract of Meliosma simplicifolia (L.) for total phenol, tannin, total flavonoid, antioxidant activity, anti-inflammatory and identify the phytoconstituents utilizing GC-MS and FT-IR. Methods: The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, superoxide radical (O2•) scavenging activity. The anti-inflammatory activity was evaluated using carrageenan and formalin-induced paw edema models using Wistar albino rats. The GC-MS and FT-IR analysis of the methanolic stem extract of M. simplicifolia was revealed the presence of phytochemicals. Results: Quantitative studies of estimated phenol, flavonoid and tannin, as for the methanol extract of stem showed the highest content of phenolic compounds (39.83±3.62GAE mg/100). Antioxidant activities were concluded the estimation M. simplicifolia stem for as followed the studies. In stem the methanol extract showed the highest DPPH scavenging activity (124.3µg/ml). The anti-inflammatory activity has shown in high doses of methanolic extract 250 mg/kg of significant value (p<0.05) inhibition of paw edema, on 6th hour, respectively. The FT-IR analysis has confirmed their characteristic peak values and functional groups. Conclusion: M. simplicifolia has an effective of anti-inflammatory activity and constitutes a potential source for the development of new treatments

PRELIMINARY SCREENING OF PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT, AND ANTI-BACTERIAL ACTIVITIES IN THE METHANOLIC LEAF EXTRACT OF MELIOSMA SIMPLICIFOLIA (L.)

Objective: In the present study is investigated of phytochemicals and antioxidant activities of the leaf extracts from Meliosma simplicifolia (L.). Methods: The seaweed sample was subjected to extraction with solvents of different polarities (hot water, methanol, acetone, chloroform, and petroleum ether) and screened for phytochemicals according to standard methods. The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, and superoxide radical (O2•) scavenging activity. The ICPMS and GC-MS analysis of the methanol leaf extract of M. simplicifolia was revealed the presence of antibacterial. Results: Phytochemical analysis revealed the presence of Preliminary metabolites Quantitative studies of estimated phenol, flavonoid and tannin, as for the methanol extract of stem showed the highest content of phenolic compounds (40.71±0.94 GAE mg/100). Antioxidant activities were concluded the estimation M. simplicifolia leaf for as followed the studies. In leaf the highest DPPH scavenging activity (132.3 μg/ml), ICP-MS analysis of the leaf extract showed the presence minerals such as Mg, Fe, Cu, Na, and Zn in excess. The leaf extract of the plant was also tested for its antibacterial activity and results confirmed that it has potential activity. Conclusion: The preliminary studies in the methanol extract of the leaf of M. simplicifolia are suggestive of the antibacterial potentials of the methanol extract of leaves of M. simplicifolia

FREE-RADICAL SCAVENGING ACTIVITY LEAF EXTRACT OF LITSEA LAEVIGATA GAMBLE

Objective: In the present study, antioxidant activity in the leaf of the pet-ether, chloroform, acetone and methanolic extracts from Litsea laevigata Gamble. Leaf was investigated by employing established in vitro studies. L. laevigata belongs to the Lauraceae family. Methods: The capability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals were determined by in vitro antioxidant assays using 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH.) scavenging, reducing power assay, superoxide radical (O2*-) scavenging activity, phosphomolybdenum assay, FRAP, ABT and metal chelating activity were performed to know the antioxidant potency of the plant extract of leaves of L. laevigata. Results: Results are evaluated higher in leaf extract of L. laevigata recorded total phenol, total flavonoid, and tannin. The present state of work was designed to evaluate the phytochemical, antioxidant in the plant leaf extracts of L. laevigata. The plant L. laevigata methanolic extract of leaf showed greater IC50 antioxidant activity of DPPH assay (5.264 µg/ml) and compare to other extract, higher phosphomolybdenum reduction (164.36 mg/g), better Reducing power activity leaf in methanol (0.711%), higher ferric reducing power (4060.66MmolFe(II)E/mg), and higher in superoxide radical scavenging activity in (78.12 mg/ml). However, the better metal chelating ability was shown by the water extracts of the leaf (5.145 EDTAE/100g) compared to other solvent extracts. Conclusion: The result indicates the total phenol and antioxidant activity potential of L. laevigata

Indigenous Knowledge on Medicinal Plants Among the Local People of Puducherry Region (Union Territory), India

A survey on the use of medicinal plants by local traditional healers was carried out in Puducherry (Union Territory) region, southern India. Findings presented in this paper were gathered by group discussions, interviews and questionnaires with the local people having knowledge of medicinal values of plants; this study involved a total of 24 plant species distributed in 22 families. These medicinal plants employed by them are listed with Latin name, family, local name, parts used, mode of preparation of the drug and medicinal uses. Generally, fresh parts of the plants are used for the preparation of medicine and leaves are most commonly used. The results of this study showed that local people still depend on plants for the cure of certain diseases

Phytochemical screening and antimicrobial activity of Capsicum chinense Jacq.

The present study investigated on the comparative evaluation of the extraction, quantification, phytochemical and antimicrobial activity of Capsaicin from acetone and acetonitrile extracts of Capsicum chinense Jacq. The polar aprotic solvent extracts showed high amount of Capsaicin with pungency level of 1,529,500 Scoville Heat Units. The Thin layer chromatography method is providing a fingerprint of plant extract. The Capsaicin extracted in the solvents on TLC chromatogram was viewed under UV 254 nm and UV 366 nm and documented. The extraction and estimation of chlorophyll and Carotenoids were also performed for the plant sample following standard procedure. Phytochemical analysis shows that acetone and acetonitrile extract of callus, leaf, shoot, fruit and seed which shows abundant presence of alkaloids, flavonoids, phenols, saphonins etc. The acetone and acetonitrile extract showed maximum zone of inhibition of Klebsiella pneumonia and Staphylococcus aureus against the gram positive and gram negative bacteria respectively through agar well diffusion method. The acetone and acetonitrile extract was found to be more effective at different concentration against all the tested bacteria and fungi. The results revealed that the Capsaicin and other secondary metabolites present in the acetone and acetonitrile extract of Capsicum chinense would contribute for the further extraction and purification of capsaicin as an antimicrobial agent

Pengaruh Akuntabilitas, Kompetensi, Profesionalisme, Integritas, dan Objektivitas Akuntan Publik terhadap Kualitas Audit dengan Independensi sebagai Variabel Moderating (Studi Empiris pada Beberapa Kap Afliasi Asing dan Kap Non Afliasi Asing di Jakarta)

This research was to obtain empirical evidence on the influence of Accountability, Competence, Professionalism, Integrity, and Objectivity of Public Accountant to the quality of audit with independence as Moderating Variable. The research was conducted by distributing questionnaires at several affiliated and non-affiliated registered public accountants located in Jakarta. Analysis modelused to test the hypotheses was multiple linear regression, these analysis was based on valid questionnaires from 117 respondents.These researchoutcomes concluded that the accountability and integrity of partially significant effect on the quality of audit, while the competence, professionalism, and objectivity had no effect on audit quality partially. In addition, this research proved that accountability moderated by theindependence significantly affected the quality of the audit, while the competence, professionalism, integrity and objectivity moderated byindependence had no significant effect on the quality of the audit

DESIGN OF LOW POWER PULSE TRIGGERED FLIP FLOP USING CONDITIONAL PULSE- ENHANCEMENT SCHEME

Volume 2 Issue 1 (January 2014

ANALYSIS OF THE PHYTOCHEMICALS, ANTIOXIDANTS, AND ANTIMICROBIAL ACTIVITY OF FICUS TSJAHELA BURM. F LEAF, BARK, AND FRUIT EXTRACTS

Objective: Ficus tsjahela Burm. f is a medicinal tree species, endemic to the Western Ghats having various healing properties. This study focused to check the antioxidant and antimicrobial activities of the leaf, bark, and fruit samples of F. tsjahela. Methods: The plant samples were subjected to Soxhlet extraction for phytochemical analysis and further experimental studies. The test on phytochemical studies indicated the presence of alkaloids, saponins, glycosides, and flavonol glycosides within the plant parts, respectively. The estimation of alkaloids, saponins, in vitro antioxidant, and antibacterial activities revealed that the methanol bark extracts have high activity compared to others. Results: Total alkaloid and saponin content was found to be high in leaf methanol extract was 996.17 mg/g quinine equivalent/g and 957.3 mg/g diosgenin equivalent/g, respectively. In vitro antioxidant assays revealed a strong radical scavenging potential of the methanol bark extract against stable (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), phosphomolybdenum, and superoxide radicals. Agar well diffusion method has been used to determine the antimicrobial activities against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli, Acetobacter aceti, and Pseudomonas aeruginosa). Conclusion: The bark methanol extract of F. tsjahela has exhibited remarkable antioxidant activity and significant antibacterial activity (p<0.05) against all tested bacterial strains observed

ASSESSMENT OF PHYTOCHEMICALS AND IN VITRO ANTIOXIDANT, ANTI-INFLAMMATORY ACTIVITY OF SARCOSTEMMA BRUNONIANUM WIGHT AND ARN

Objective: Sarcostemma brunonianum Wight and Arn is a potential medicinal plant belonging to Asclepiadaceae. Bioactive constituents of the plant support the application of treating various ailments in the traditional system of medicine. The study aims to determine the presence of various phytoconstituents in stem, root, and flowers. Methods: Hot percolation method was carried out to obtain crude extracts using different solvent systems from low polar-high polar solvents ranging from petroleum ether, chloroform, (mid-polar) ethyl acetate, methanol and water. Estimation of total phenols, tannins and In vitro antioxidant, anti-inflammatory activities were evaluated for the determination of potential pharmaceutical drugs. Results: The results revealed the presence of some phytoconstituents such as phenols, tannins, glycosides, gums and mucilages. Ascorbic acid, BSA, Rutin and Gallic acid were used as the reference standard. The total phenolic content was found to be high in stem methanol extract 440.84±69.99 mg/g Gallic acid equivalent, whereas the tannin content was 291.78±4.68 mg/g GAE. The result proves that the S. brunonianum stem methanol extract possesses antioxidant and anti-inflammatory activities when compared to reference standards. In vitro, Nitric oxide scavenging activity of stem showed a maximum % of inhibition in methanol stem extract (24.39µg/ml) and anti-inflammatory activity maximum inhibition was found to be (55.56 %) in stem methanol and flower(53.62 %). The IC50 (concentration required for 50% inhibition) was also calculated for the DPPH radical model. Conclusion: This study results proclaims and justifies the role of folklore medicinal plant S. brunonianum in the treatment of inflammatory-related ailments and can be recommended for an effective drug