Infection Prevention and the Protective Effects of Unidirectional Displacement Flow Ventilation in the Turbulent Spaces of the Operating Room

Background: Unidirectional displacement flow (UDF) ventilation systems in operating rooms are characterized by a uniformity of velocity 80% and protect patients and operating room personnel against exposure to hazardous substances. However, the air below the surgical lights and in the surrounding zone is turbulent, which impairs the ventilation system’s effect. Aim: We first used the recovery time (RT) as specified in International Organization for Standardization 14644 to determine the particle reduction capacity in the turbulent spaces of an operating room with a UDF system. Methods: The uniformity of velocity was analyzed by comfort-level probe grid measurements in the protected area below a hemispherical closed-shaped and a semi-open column-shaped surgical light (tilt angles: 0/15/30) and in the surrounding zone of a research operating room. Thereafter, RTs were calculated. Results: At a supply air volume of 10,500 m3/h, the velocity, reported as average uniformity+standard deviation, was uniform in the protected area without lights (95.8% + 1.7%), but locally turbulent below the hemispherical closedshaped (69.3% + 14.6%), the semi-open column-shaped light (66.9% + 10.9%), and in the surrounding zone (51.5%+17.6%). The RTs ranged between 1.1 and 1.7 min below the lights and 3.5+0.28 min in the surrounding zone and depended exponentially on the volume flow rate. Conclusions: Compared to an RT of 20 min as required for operating rooms with mixed dilution flow, particles here were eliminated 12–18 times more quickly from below the surgical lights and 5.7 times from the surrounding zone. Thus, the effect of the lights was negligible and the UDF’s retained its strong protective effect

Ion Collisions in Very Strong Electric Fields

A Classical Trajectory Monte Carlo (CTMC) simulation has been made of processes of charge exchange and ionization between an hydrogen atom and fully stripped ions embedded in very strong static electric fields ($O(10^{10}$ V/m$)$), which are thought to exist in cosmic and laser--produced plasmas. Calculations show that the presence of the field affects absolute values of the cross sections, enhancing ionization and reducing charge exchange. Moreover, the overall effect depends upon the relative orientation between the field and the nuclear motion. Other features of a null-field situation, such as scaling laws, are revisited.Comment: Latex, 13 pages, 11 figures (available upon request), to be published in Journal of Physics

Self-consistent solution for the polarized vacuum in a no-photon QED model

We study the Bogoliubov-Dirac-Fock model introduced by Chaix and Iracane ({\it J. Phys. B.}, 22, 3791--3814, 1989) which is a mean-field theory deduced from no-photon QED. The associated functional is bounded from below. In the presence of an external field, a minimizer, if it exists, is interpreted as the polarized vacuum and it solves a self-consistent equation. In a recent paper math-ph/0403005, we proved the convergence of the iterative fixed-point scheme naturally associated with this equation to a global minimizer of the BDF functional, under some restrictive conditions on the external potential, the ultraviolet cut-off $\Lambda$ and the bare fine structure constant $\alpha$. In the present work, we improve this result by showing the existence of the minimizer by a variational method, for any cut-off $\Lambda$ and without any constraint on the external field. We also study the behaviour of the minimizer as $\Lambda$ goes to infinity and show that the theory is "nullified" in that limit, as predicted first by Landau: the vacuum totally kills the external potential. Therefore the limit case of an infinite cut-off makes no sense both from a physical and mathematical point of view. Finally, we perform a charge and density renormalization scheme applying simultaneously to all orders of the fine structure constant $\alpha$, on a simplified model where the exchange term is neglected.Comment: Final version, to appear in J. Phys. A: Math. Ge

New Results on Pb-Au Collisions at 40 AGeV from the CERES/NA45 Experiment

In 1999 the CERES/NA45 ran at the CERN SPS with a beam energy of 40 GeV/nucleon. The data set comprises about 8.7 millions Pb-Au events with a trigger selection corresponding to approximately the most central 30% of the geometrical cross section. Results on low-mass electron pair analysis are presented. The upgrade of the experimental setup with the radial drift TPC has allowed to enhance hadron physics capabilities of the experiment. New results on hadron spectra (including Lambda) and flow are presented.Comment: Talk at the International Nuclear Physics Conference INPC2001, Berkeley, CA, July 29th - August 3rd 200

e+e--pair production in Pb-Au collisions at 158 GeV per nucleon

We present the combined results on electron-pair production in 158 GeV/n {Pb-Au} ($\sqrt{s}$= 17.2 GeV) collisions taken at the CERN SPS in 1995 and 1996, and give a detailed account of the data analysis. The enhancement over the reference of neutral meson decays amounts to a factor of 2.31$\pm0.19 (stat.)\pm0.55 (syst.)\pm0.69 (decays)$ for semi-central collisions (28% $\sigma/\sigma_{geo}$) when yields are integrated over $m>$ 200 MeV/$c^2$ in invariant mass. The measured yield, its stronger-than-linear scaling with $N_{ch}$, and the dominance of low pair $p_t$ strongly suggest an interpretation as {\it thermal radiation} from pion annihilation in the hadronic fireball. The shape of the excess centring at $m\approx$ 500 MeV/$c^2$, however, cannot be described without strong medium modifications of the $\rho$ meson. The results are put into perspective by comparison to predictions from Brown-Rho scaling governed by chiral symmetry restoration, and from the spectral-function many-body treatment in which the approach to the phase boundary is less explicit.Comment: 39 pages, 40 figures, to appear in Eur.Phys.J.C. (2005

Flow and non-flow event anisotropies at the SPS

A study of differential elliptic event anisotropies (v_2) of charged particles and high-pt pions in 158 AGeV/c Pb+Au collisions is presented. Results from correlations with respect to the event plane and from two-particle azimuthal correlations are compared. The latter give systematically higher v_2 values at pt>1.2GeV/c providing possibly an evidence of a non-flow semihard component.Comment: 4 pages, 6 figures, Quark Matter 2002, Nantes, to appear in Nucl. Phys.

Event-by-event fluctuations of the mean transverse momentum in 40, 80, and 158 A GeV/c Pb-Au collisions

Measurements of event-by-event fluctuations of the mean transverse momentum in Pb-Au collisions at 40, 80, and 158 A GeV/c are presented. A significant excess of mean p_T fluctuations at mid-rapidity is observed over the expectation from statistically independent particle emission. The results are somewhat smaller than recent measurements at RHIC. A possible non-monotonic behaviour of the mean p_T fluctuations as function of collision energy, which may have indicated that the system has passed the critical point of the QCD phase diagram in the range of mu_B under investigation, has not been observed. The centrality dependence of mean p_T fluctuations in Pb-Au is consistent with an extrapolation from pp collisions assuming that the non-statistical fluctuations scale with multiplicity. The results are compared to calculations by the RQMD and UrQMD event generators.Comment: 28 pages, 10 figure

In-vitro model systems to study Hepatitis C Virus

Hepatitis C virus (HCV) is a major cause of chronic liver diseases including steatosis, cirrhosis and hepatocellular carcinoma. Currently, there is no vaccine available for prevention of HCV infection due to high degree of strain variation. The current treatment of care, Pegylated interferon α in combination with ribavirin is costly, has significant side effects and fails to cure about half of all infections. The development of in-vitro models such as HCV infection system, HCV sub-genomic replicon, HCV producing pseudoparticles (HCVpp) and infectious HCV virion provide an important tool to develop new antiviral drugs of different targets against HCV. These models also play an important role to study virus lifecycle such as virus entry, endocytosis, replication, release and HCV induced pathogenesis. This review summarizes the most important in-vitro models currently used to study future HCV research as well as drug design

MHC Class I Endosomal and Lysosomal Trafficking Coincides with Exogenous Antigen Loading in Dendritic Cells

BACKGROUND: Cross-presentation by dendritic cells (DCs) is a crucial prerequisite for effective priming of cytotoxic T-cell responses against bacterial, viral and tumor antigens; however, this antigen presentation pathway remains poorly defined. METHODOLOGY/PRINCIPAL FINDINGS: In order to develop a comprehensive understanding of this process, we tested the hypothesis that the internalization of MHC class I molecules (MHC-I) from the cell surface is directly involved in cross-presentation pathway and the loading of antigenic peptides. Here we provide the first examination of the internalization of MHC-I in DCs and we demonstrate that the cytoplasmic domain of MHC-I appears to act as an addressin domain to route MHC-I to both endosomal and lysosomal compartments of DCs, where it is demonstrated that loading of peptides derived from exogenously-derived proteins occurs. Furthermore, by chasing MHC-I from the cell surface of normal and transgenic DCs expressing mutant forms of MHC-I, we observe that a tyrosine-based endocytic trafficking motif is required for the constitutive internalization of MHC-I molecules from the cell surface into early endosomes and subsequently deep into lysosomal peptide-loading compartments. Finally, our data support the concept that multiple pathways of peptide loading of cross-presented antigens may exist depending on the chemical nature and size of the antigen requiring processing. CONCLUSIONS/SIGNIFICANCE: We conclude that DCs have 'hijacked' and adapted a common vacuolar/endocytic intracellular trafficking pathway to facilitate MHC I access to the endosomal and lysosomal compartments where antigen processing and loading and antigen cross-presentation takes place

Enhanced production of low-mass electron-positron pairs in 40-AGeV Pb-Au collisions at the CERN SPS

We report on first measurements of low-mass electron pairs in Pb-Au collisions at the lower SPS beam energy of 40 AGeV. The pair yield integrated over the range of invariant masses 0.2 e+ e- annihilation with a modified rho-propagator. They may be linked to chiral symmetry restoration and support the notion that the in-medium modifications of the rho are more driven by baryon density than by temperature.We report on first measurements of low-mass electron pairs in Pb-Au collisions at the lower SPS beam energy of 40 AGeV. The pair yield integrated over the range of invariant masses 0.2<m<1 GeV/c^2 is enhanced by a factor of 5.1+-1.3(stat)+-1.0/1.5(syst data/decays) over the expectation from neutral meson decays, more than previously observed at the higher energy of 158 AGeV. The results are discussed with reference to model calculations based on pi+ pi- -> e+ e- annihilation with a modified rho-propagator. They may be linked to chiral symmetry restoration and support the notion that the in-medium modifications of the rho are more driven by baryon density than by temperature