Spectral shift as advanced index for fruit chlorophyll breakdown

The decline of fruit chlorophyll is a valuable indicator of fruit ripeness. Fruit chlorophyll content can be nondestructively estimated by UV/VIS spectroscopy at fixed wavelengths. However, this approach cannot explain the complex changes in chlorophyll catabolism during fruit ripening. We introduce the apparent peak position of the red band chlorophyll absorption as a new qualitative spectral indicator. Climacteric fruit (apple: n = 24, mango: n = 38, tomato: n = 48) were analysed at different ripeness stages. The peak position and corresponding intensity values were determined between 650 and 690 nm of nondestructively measured fruit spectra as well as of corresponding spectra of fruit extracts. In the extracts, individual contents of chlorophyll a, chlorophyll b, pheophytin a and carotenoids were analysed photometrically, using an established iterative multiple linear regression approach. Nondestructively measured peak positions shifted unimodal in all three fruit species with significant shifts between fruit ripeness classes of maximal 2.00 ± 0.27 nm (mean ± standard error) in tomato and 0.57 ± 0.11 nm in apple. Peak positions in extract spectra were related to varying pigment ratios (Rmax = −0.91), considering individual pigments in the pool. The peak intensities in both spectral readings, nondestructive and fruit extracts, were correlated with absolute chlorophyll contents with Rmax = −0.84 and Rmax = 1.00, respectively. The introduced spectral marker of the apparent peak position of chlorophyll absorbance bears the potential for an advanced information gain from nondestructive spectra for the determination of fruit ripeness

Optical properties of developing pip and stone fruit reveal underlying structural changes

Analyzing the optical properties of fruits represents a powerful approach for non-destructive observations of fruit development. With classical spectroscopy in the visible and near-infrared wavelength ranges, the apparent attenuation of light results from its absorption or scattering. In horticultural applications, frequently, the normalized difference vegetation index (NDVI) is employed to reduce the effects of varying scattering properties on the apparent signal. However, this simple approach appears to be limited. In the laboratory, with time-resolved reflectance spectroscopy, the absorption coefficient, μa, and the reduced scattering coefficient, μs′, can be analyzed separately. In this study, these differentiated optical properties were recorded (540-940 nm), probing fruit tissue from the skin up to 2 cm depth in apple (Malus × domestica 'Elstar') and plum (Prunus domestica 'Tophit plus') harvested four times (65-145 days after full bloom). The μa spectra showed typical peak at 670 nm of the chlorophyll absorption. The μs′ at 670 nm in apple changed by 14.7% (18.2-15.5 cm-1), while in plum differences of 41.5% (8.5-5.0 cm-1) were found. The scattering power, the relative change of μs′, was zero in apple, but enhanced in plum over the fruit development period. This mirrors more isotropic and constant structures in apple compared with plum. For horticultural applications, the larger variability in scattering properties of plum explains the discrepancy between commercially assessed NDVI values or similar indices and the absolute μa values in plum (R < 0.05), while the NDVI approach appeared reasonable in apple (R ≥ 0.80)

A Worker-Like Female of Myrmica sabuleti (Meinert, 1861) (Hymenoptera: Formicidae: Myrmicinae) in a Pitfall Trap with Five Mermithids (Nematoda: Mermithidae) Protruding from the Gaster

A worker-like female of Myrmica sabuleti (Meinert, 1861), pitfall-trapped near Jena, Germany, in late summer 2016, was infested by five postparasitic juvenile mermithids. They poked out of the ant´s gaster as a trail of seven filaments of various lengths. Apart from its swollen gaster, the ant differed from conspecifics in several morphometric parameters. Using both morphological and molecular techniques, the parasite family Mermithidae was confirmed. Our stray find raises multiple questions concerning the genus and species identity of the parasite, its biology, and the infestation rate of the host ant population. More mermithid awareness by the various researchers working with Myrmica will help, but directed fieldwork, experimental life-history research, and molecular studies are needed to emancipate progress in ant-mermithid research from serendipity

Courtship with two spoons—Anatomy and presumed function of the bizarre antennae of Cardiocondyla zoserka ant males

Mating in ants often occurs on the wing during nuptial flights or on the ground when scattered female sexuals attract males by pheromones. In both scenarios, there is little opportunity for males to engage in prolonged aggressive competition or elaborate courtship displays. Male morphology is therefore adapted to locating female sexuals and mating, and it lacks specific weapons or other traits associated with courtship. In contrast, sexuals of the ant genus Cardiocondyla typically mate in their natal nests. As a consequence, in many species winged males have been replaced by wingless fighter or territorial males, which kill or expel rival males with their strong mandibles and show complex mating behavior. However, no wingless males are known from Cardiocondyla zoserka from West Africa, and instead, winged males have evolved a bizarre secondary sexual trait: uniquely shaped antennae with spoon-like tips that show heavily sculptured ventral surfaces with numerous invaginations. We here report on the courtship behavior of C. zoserka males and describe antennal glands with class 3 gland cells, which presumably secrete a close range sex pheromone. Antennal glands have not yet been found in males of other ant species, including a close relative of C. zoserka, suggesting that in ants with intranidal mating sexual selection can rapidly lead to highly divergent adaptations and the evolution of novel structures

Permafrost-related research data - their accessibility, visualization, and publication using GIS and WebGIS technology

Permafrost regions are highly sensitive to climate changes. The monitoring of key variables and identification of relevant-processes is of topmost importance in these environments. ESA DUE GlobPermafrost (www.globpermafrost.info) provides a remote sensing data service for permafrost research and applications. This service was extended by permafrost modelling (time series), implemented in the new ESA CCI+ Permafrost project (2018-2021). The service comprises of the generation of remote sensing products for various regions and spatial scales as well as the specific infrastructures for visualisation, dissemination and access to datasets - PerSys. PerSys is the ESA GlobPermafrost geospatial information service for publishing and visualisation of information and data products to the public. Data products are described and searchable in the PerSys data catalogue (apgc.awi.de), and data visualisation employs the AWI WebGIS-infrastructure maps@awi (http://maps.awi.de), a highly scalable data visualisation unit within the AWI data-workflow framework O2A, from Observation to Archive. maps@awi WebGIS technology supports the project-specific visualisation of raster and vector data products of any spatial resolution and remote sensing origin. This is a prerequisite for the visualisation of the wide range of GlobPermafrost remote sensing products like: Landsat multispectral index trends (Tasseled Cap Brightness, Greeness, Wetness; Normalized Vegetation Index NDVI), Arctic land cover (e.g. shrub height, vegetation composition), lake ice grounding, InSAR-based land surface deformation, rock glacier velocities and a spatially distributed permafrost model output with permafrost probability and ground temperature per pixel. We established several WebGIS projects for the adaption to products specific spatial scales. For example, the WebGIS ‘Arctic’ visualises the Circum-Artic products. Highly resolved data products for rock glacier movements are visualised on regional scales in the WebGIS projects ‘Alps’, ‘Andes’ or ‘Central Asia’. The PerSYS WebGIS also visualises the stations of the WMO GCOS ground monitoring networks of the permafrost community: the Global Terrestrial Network for Permafrost GTN-P managed by the International Permafrost Association IPA. The PerSYS WebGIS has been continuously adapted in close co-operation with user at user workshops and at conferences and the International Permafrost Association (IPA)

Activation of executioner caspases is a predictor of progression-free survival in glioblastoma patients: a systems medicine approach.

Glioblastoma (GBM) is the most common and aggressive primary brain tumor in adults. GBM cells are highly resistant to apoptosis induced by antitumor drugs and radiotherapy resulting in cancer progression. We assessed whether a systems medicine approach, analysing the ability of tumor cells to execute apoptosis could be utilized to predict the response of GBM patients to treatment. Concentrations of the key proapoptotic proteins procaspase-3, procaspase-9, Smac and Apaf-1 and the antiapopotic protein XIAP were determined in a panel of GBM cell lines and GBM patient tumor resections. These values were used as input for APOPTO-CELL, a systems biological based mathematical model built to predict cellular susceptibility to undergo caspase activation. The modeling was capable of accurately distinguishing between GBM cells that die or survive in response to treatment with temozolomide in 10 of the 11 lines analysed. Importantly the results obtained using GBM patient samples show that APOPTO-CELL was capable of stratifying patients according to their progression-free survival times and predicted the ability of tumor cells to support caspase activation in 16 of the 21 GBM patients analysed. Calculating the susceptibility to apoptosis execution may be a potent tool in predicting GBM patient therapy responsiveness and may allow for the use of APOPTO-CELL in a clinical setting

ESA GlobPermafrost - WebGIS based Visualisation of Remote Sensing Data

GIS server and desktop GIS technologies support scientific work at all levels, from data collection and data processing to data management and data visualisation. Here we present how the development and publication of scalable WebGIS data services supports the ESA DUE Globpermafrost (2016-2018), and the ESA CCI+ Permafrost (2018-2021) projects, specifically in the interaction with the permafrost community. Within ESA DUE programs, user feedback is essential to improve the remote sensing products. This is why ESA GlobPermafrost had to focus on methods and infrastructure for data presentation, and established PerSYS (Permafrost Information System). PerSYS became the ESA GlobPermafrost geospatial information service for publishing and visualisation of information and data products to the public. Data products are described and searchable in the PerSYS Data Catalogue, a core component of the Arctic Permafrost Geospatial Centre (APGC), established within the framework of ERC PETA-CARB at AWI. All GlobPermafrost data products will be DOI-registered and archived in the data archive PANGAEA provided by AWI. The data visualisation employs AWI’s WebGIS-infrastructure maps@awi (http://maps.awi.de), a highly scalable data visualisation unit within AWI’s data workflow framework O2A (from Observation to Archive). GIS services have been created and designed using ArcGIS for Desktop (latest Version) and finally published as a Web Map Service (WMS), an internationally standardized format (Open Geospatial Consortium (OGC)), using ArcGIS for Server. The project-specific data WMS as well as a resolution-specific background map WMS are embedded into a GIS viewer application based on Leaflet, an open-source JavaScript library. The GIS viewer application was adapted to interlink all GlobPermafrost WebGIS projects, and especially to enable their direct accessibility via the GlobPermafrost Overview WebGIS. The PerSys WebGIS is accessible via the GlobPermafrost project webpage and linked to the respective product groups as well as to maps@awi. WebGIS technology within maps@awi supports the project-specific visualisation of raster and vector data products of diverse spatial resolutions and remote sensing sources. This is a prerequisite for the visualisation of the wide range of GlobPermafrost remote sensing products like: Landsat multispectral index trends (Tasseled Cap Brightness, Greeness, Wetness; Normalized Vegetation Index NDVI), Arctic land cover (e.g., shrub height, vegetation composition), lake ice grounding, InSAR-based land surface deformation, rock glacier velocities and a spatially distributed permafrost model output with permafrost probability and ground temperature per pixel. All WebGIS projects are adapted to the products’ specific spatial scales. For example, the WebGIS ‘Arctic’ visualises the Circum-Artic products. Higher spatial resolution products for rock glacier movements are visualised on regional scales in the WebGIS projects ‘Alps’, ‘Andes’ or ‘Central Asia’. The PerSYS WebGIS also visualises the locations of the WMO GCOS ground monitoring networks of the permafrost community: the Global Terrestrial Network for Permafrost GTN-P managed by the International Permafrost Association IPA. The PerSYS WebGIS has been presented on several User workshops and at conferences, and is being continuously adapted in close interaction with the IPA

Leipziger und Schkeuditzer Gewässer: 24 Fließgewässer im Portrait

Der Auwald und seine Gewässer schlängeln sich wie grüne und blaue Bänder durch die Stadt Leipzig und ihre Umgebung

PerSys - WebGIS-based permafrost data visualisation system for ESA GlobPermafrost

ESA DUE GlobPermafrost (www.globpermafrost.info) provides a remote sensing data service for permafrost research and applications. This service comprises of the generation of remote sensing products for various regions and spatial scales, and specific infrastructures for visualisation, dissemination and access to datasets. PerSys is the ESA GlobPermafrost geospatial information service for publishing and visualisation of information and data productstothepublic.DataproductsaredescribedandsearchableinthePerSysDataCatalogue,acorecomponent of the Arctic Permafrost Geospatial Centre (APGC), established within the framework of ERC PETA-CARB at AWI. The data visualisation employs the AWI WebGIS-infrastructure maps@awi (http://maps.awi.de), a highly scalable data visualisation unit within the AWI data-workflow framework O2A, from Observation to Archive. WebGIS technology in maps@awi supports the project-specific visualisation of raster and vector data products of diverse spatial resolutions and remote sensing sources. This is a prerequisite for the visualisation of the wide range of GlobPermafrost remote sensing products like: Landsat multispectral index trends (Tasseled Cap Brightness, Greeness, Wetness; Normalized Vegetation Index NDVI), Arctic land cover (e.g., shrub height, vegetation composition), lake ice grounding, InSAR-based land surface deformation, rock glacier velocities and a spatially distributed permafrost model output with permafrost probability and ground temperature per pixel. All WebGIS projects are adapted to the products specific spatial scale. For example, the WebGIS ‘Arctic’ visualises the Circum-Artic products. Higher spatial resolution products for rock glacier movements are visualised on regional scales in the WebGIS projects ‘Alps’, ‘Andes’ and ‘Central Asia’. GIS services were created and designed using ArcGIS for Desktop (10.4) and finally published as a Web MapService(WMS),aninternationallystandardizedformat(OpenGeospatialConsortium(OGC)),usingArcGIS for Server (10.4). The project-specific data WMS as well as a resolution-specific background map WMS are embedded into a GIS viewer application based on Leaflet, an open-source JavaScript library. The GIS viewer application was adapted to interlink all WebGIS projects, and especially to enable their direct accessibility via the GlobPermafrost Overview WebGIS project. The PerSys WebGIS is accessible via the GlobPermafrost project webpage and linked to the respective product groups as well as on maps@awi (maps.awi.de). All GlobPermafrost data products will be DOI-registered and archived in PANGAEA. In future, PerSys intends to encourage permafrost researchers other than GlobPermafrost to integrate and visualise their dat

Staphylococcus aureus nasal colonization among dental health care workers in Northern Germany (StaphDent study)

Methicillin-resistant Staphylococcus aureus (MRSA) can colonize dental patients and students, however, studies on the prevalence of MRSA and methicillin-susceptible S. aureus (MSSA) among dental health care workers (DHCW) including use of personal protective equipment (PPE) are scarce. We conducted an observational study (StaphDent study) to (I) determine the prevalence of MRSA and MSSA colonization in DHCW in the region of Mecklenburg Western-Pomerania, Germany, (II) resolve the MSSA population structure to gain hints on possible transmission events between co-workers, and (III) clarify use of PPE. Nasal swabs were obtained from dentists (n?=?149), dental assistants (n?=?297) and other dental practice staff (n?=?38). Clonal relatedness of MSSA isolates was investigated using spa typing and, in some cases, whole genome sequencing (WGS). PPE use was assessed by questionnaire. While 22.3% (108/485) of the participants were colonized with MSSA, MRSA was not detected. MSSA prevalence was not associated with size of dental practices, gender, age, or duration of employment. The identified 61 spa types grouped into 17 clonal complexes and four sequence types. Most spa types (n?=?51) were identified only once. In ten dental practices one spa type occurred twice. WGS data analysis confirmed a close clonal relationship for 4/10 isolate pairs. PPE was regularly used by most dentists and assistants. To conclude, the failure to recover MRSA from DHCW reflects the low MRSA prevalence in this region. Widespread PPE use suggests adherence to routine hygiene protocols. Compared to other regional HCW MRSA rates the consequent usage of PPE seems to be protective