Isolation and characterization of homokaryotic strains from the ligninolytic basidiomycete Ceriporiopsis subvermispora

Indexación: Scopus.Genetic analyses of the lignin-degrading fungus Ceriporiopsis subvermispora is complicated by a dikaryotic nuclear condition and the absence of spore forms. Previous investigations had identified a family of closely related sequences encoding manganese peroxidase (MnP), but the relationship between genes and allelic variants could not be experimentally established. Addressing this issue, homokaryotic derivatives of C. subvermipora strain FP105752 were isolated from regenerated protoplasts. Designated CsA and CsB, their homokaryotic nature was established by polymerase chain reaction amplification and sequence analysis of the allelic variants of three MnP genes. Isoelectrofocusing revealed fewer MnP isoenzymes in filtrates of homokaryon cultures relative to the parental strain. The homokaryotic strains will simplify genetic analyses, particularly the identification of new genes.https://academic-oup-com.recursosbiblioteca.unab.cl/femsle/article/199/1/91/55387

Molecular analysis of Broussonetia papyrifera (L.) Vent. (Magnoliophyta: Urticales) from the Pacific, based on ribosomal sequences of nuclear DNA

Broussonetia papyrifera (L.) Vent. (Magnoliophyta: Urticales), or paper mulberry, is a species of Asian origin dispersed by humans throughout the Pacific. Our aim is to evaluate the genetic variability of this plant in order to determine its potential as a commensal species for studying the mobility and/or migratory movements of the people that carried it. For this study, we analysed the non-coding transcribed spacer sequences (ITS) of ribosomal nuclear DNA found in samples of B. papyrifera collected in Remote Oceania and Taiwan. Our results show three genotypes: the Pacific samples form a distinct and homogenous subgroup, while the Taiwanese accessions present two genotypes. We discuss the relevance of these results in the context of the dispersal of B. papyrifera in the Pacific and its association with Austronesian migration history. © 2011 The Royal Society of New Zealand

Paper mulberry (Broussonetia papyrifera) as a commensal model for human mobility in Oceania: anthropological, botanical and genetic considerations

Paper mulberry (Broussonetia papyrifera (L.) Vent.) was one of the most widely distributed crop species in prehistoric Oceania, occurring from continental East Asia to the Polynesian islands. Its broad distribution is largely due to human-mediated dispersal during colonization of the islands of Near and Remote Oceania. We explore the potential for analyses of genetic variation in paper mulberry and the value of such data for the development of a new commensal model species for reconstructing patterns of human mobility in Oceania. We introduce and discuss paper mulberry as another commensal species and outline key features for its contribution to the understanding of human migration and post-colonization interaction. Here, we describe some of the extant B. papyrifera populations in Remote Oceania and Taiwan that were sampled for initial studies. We argue that the unique characteristics of this species and its importance in ancient Pacific island societies may provide the opportunity to collect valuable genetic data with which we can address several key questions in Pacific prehistory

Ancient and modern introduction of <i>Broussonetia papyrifera</i> ([L.] Vent.; Moraceae) into the Pacific: genetic, geographical and historical evidence

<div><p><i>Broussonetia papyrifera</i> (L.) Vent. (Moraceae), or paper mulberry, is a species of cultural importance in South East Asia, East Asia and the Pacific. Originally from mainland South East Asia or East Asia, this plant was introduced into the Pacific range by prehistoric Austronesian voyagers. We used non-coding internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA and inter-simple sequence repeat (ISSR) on 79 samples of <i>B. papyrifera</i> from different islands of Remote Oceania, and South East Asia and East Asia. Our results show an absence of genetic diversity in the introduced range of Remote Oceania, with the sole exception of Hawaii. By contrast, Asian samples show genetic diversity. The data obtained suggest a prehistoric human-mediated introduction of this species from East Asia to Remote Oceania and a second, possibly historic, human-mediated introduction to Hawaii.</p></div

Association of the intronic polymorphism rs891512 (G24943A) of the endothelial nitric oxide synthase gene with hypertension in Chilean type 2 diabetes patients

We investigated two single nucleotide polymorphisms of the NOS3 gene in type 2 diabetic patients (n= 93) and healthy non-diabetic controls (n= 76) and their relationship with smoking habits, body mass index, hypertension and dyslipidemia. Results showed that eNOS polymorphism rs891512 (G24943A) is associated with hypertension in Chilean individuals (p&lt; 0.05). © 2012 Elsevier Ireland Ltd