Outcomes following abdominoperineal resection 6 years retrospective study at a rural district general hospital

Background: A range of surgical techniques are used for perineal wound closure following Abdominoperineal Excision of the Rectum (APER). The aim of this study was to assess the safety and effectiveness of using a biological mesh for perineal wound closure and to compare the outcomes following conventional suture and mesh closure of the perineal wound.Methods: A single-centre retrospective study of a cohort of patients undergoing surgery for low rectal cancer between January 2013 and December 2018. Patient records were analysed for outcomes including perineal complication rates, length of hospital stay and impact of patient factors on complication rates in mesh vs no mesh group.Results: Of the total 43 patients included in the study, 13 (30%) had a conventional perineal closure whereas 30 patients (70%) had a biological mesh reconstruction.  Early perineal wound complications were seen in 21/43 (49%) patients. Of those, 6 (29%) patients were in the no mesh group compared to 15 (71%) patients in the mesh group (p = 0.81). 84% of the patients who received neo adjuvant radiotherapy (NART) developed perineal wound infection. There was no statistically significant difference in the mesh and no mesh groups. None of the patient factors, other than preoperative anaemia, had a statistically significant association with the rate of complications in either of the groups.Conclusions: There was no statistically significant difference in the complication rate between primary and biological mesh closure. Biological mesh is safe for perineal reconstruction following APER

Versuche mit Milasana@ und B.brevis auf Nebeneffekte gegen Typhlodromus pyri (Acari, Phytoseiidae) und Aphidius rhopalosiphi (Hymenoptera, Braconidae)

Data from laboratory range-finder and field test indicate that both biological agents, Milsana (VP 2000) and B. brevis WT, can be judged well-suitable for use in organic or integrated control systems. There were no negative effects recorded against Typhlodromus pyri (Laboratory and field) and Aphidius rhopalosiphi (Iaboratory). For further confirmation, limit tests under GLP (with the highest tested concentration) will be performed in the near future. When Myco-Sin was applied in combination with Milsana in the field, population levels of T. pyri were raised compared to the control

A review of methods for detecting rats at low densities, with implications for surveillance

Invasive rats are the biggest threat to island biodiversity world-wide. Though the ecological impacts of rats on insular biota are well documented, introduced rats present a difficult problem for detection and management. In recent decades, improved approaches have allowed for island-wide eradications of invasive rats on small-medium sized islands and suppression on large islands, although both these still represent a formidable logistical and financial challenge. A key aspect of eradication or suppression and ongoing management is the ability to detect the presence of rats, especially at low densities. Here we review recent developments in the field of rat surveillance and summarise current published literature to recommend practices and the factors to consider when developing a surveillance program for either eradication or suppression plans. Of 51 empirical studies covering 17 countries, 58% were from New Zealand. Although detecting rats at low density is extremely challenging, advances over the past 15 years, have significantly improved our ability to detect rats. Motion-sensored cameras and rodent detection dogs have greatly improved our ability to detect rats at low densities, with cameras consistently showing an ability to detect rats at lower densities than other techniques. Rodent detection dogs are also able to reliably detect even an individual rat, although there are challenges to their widespread adoption, particularly in developing countries, due to the cost and skills required for their training and maintenance. New monitoring devices, the use of eDNA and drones represent current and future innovations to improve detection

Hall mobilities and sheet carrier densities in a single LiNbO3_3 conductive ferroelectric domain wall

For the last decade, conductive domain walls (CDWs) in single crystals of the uniaxial model ferroelectric lithium niobate (LiNbO$_3$, LNO) have shown to reach resistances more than 10 orders of magnitude lower as compared to the surrounding bulk, with charge carriers being firmly confined to sheets of a few nanometers in width. LNO thus currently witnesses an increased attention since bearing the potential for variably designing room-temperature nanoelectronic circuits and devices based on such CDWs. In this context, the reliable determination of the fundamental transport parameters of LNO CDWs, in particular the 2D charge carrier density $n_{2D}$ and the Hall mobility $\mu_{H}$ of the majority carriers, are of highest interest. In this contribution, we present and apply a robust and easy-to-prepare Hall-effect measurement setup by adapting the standard 4-probe van-der-Pauw method to contact a single, hexagonally-shaped domain wall that fully penetrates the 200-$\mu$m-thick LNO bulk single crystal. We then determine $n_{2D}$ and $\mu_{H}$ for a set of external magnetic fields $B$ and prove the expected cosine-like angular dependence of the Hall voltage. Lastly, we present photo-Hall measurements of one and the same DW, by determining the impact of super-bandgap illumination on the 2D charge carrier density $n_{2D}$

Adhesion between oppositely-charged polyelectrolytes

The adhesion between a grafted polyelectrolyte layer (brush) and a gel of an oppositely charged polyelectrolyte has been measured as a function of applied pressure, and the interface has been traced using neutron reflectometry. The interface (in aqueous medium at pH 6) between the (polycationic) brush and the (polyanionic) gel has a limited pressure-dependence, with a small amount of deformation of the interface at the brush-gel contact. Brushes with a dry thickness of up to 13 nm exhibit weak adhesion (measured using a mechanical force tester) with an adhesive failure when the gel is detached. Thicker brushes result in the gel exhibiting cohesive failure. Reversing the geometry, whereby a polycationic brush is replaced with a polyanion and the polyanionic gel is replaced with a polycation reveals that the pH-dependence of the adhesion is moderately symmetric about pH 6, but that the maximum force required to separate the polycation gel from the polyanion brush over the range of pH is greater than that for the polycation brush and polyanion gel. The polyanion used is poly(methacrylic acid) (PMAA) and polycations of poly[2-(diethyl amino)ethyl methacrylate] (PDEAEMA) and poly[2-(dimethyl amino)ethyl methacrylate] (PDMAEMA) were used

High definition video loggers provide new insights into behaviour, physiology, and the oceanic habitat of a marine predator, the yellow-eyed penguin

Camera loggers are increasingly used to examine behavioural aspects of free-ranging animals. However, often video loggers are deployed with a focus on specific behavioural traits utilizing small cameras with a limited field of view, poor light performance and video quality. Yet rapid developments in consumer electronics provide new devices with much improved visual data allowing a wider scope for studies employing this novel methodology. We developed a camera logger that records full HD video through a wide-angle lens, providing high resolution footage with a greater field of view than other camera loggers. The main goal was to assess the suitability of this type of camera for the analysis of various aspects of the foraging ecology of a marine predator, the yellow-eyed penguin in New Zealand. Frame-by-frame analysis allowed accurate timing of prey pursuits and time spent over certain seafloor types. The recorded video footage showed that prey species were associated with certain seafloor types, revealed different predator evasion strategies by benthic fishes, and highlighted varying energetic consequences for penguins pursuing certain types of prey. Other aspects that could be analysed were the timing of breathing intervals between dives and observe exhalation events during prey pursuits, a previously undescribed behaviour. Screen overlays facilitated analysis of flipper angles and beat frequencies throughout various stages of the dive cycle. Flipper movement analysis confirmed decreasing effort during descent phases as the bird gained depth, and that ascent was principally passive. Breathing episodes between dives were short (<1 s) while the majority of the time was devoted to subsurface scanning with a submerged head. Video data recorded on free-ranging animals not only provide a wealth of information recorded from a single deployment but also necessitate new approaches with regards to analysis of visual data. Here, we demonstrate the diversity of information that can be gleaned from video logger data, if devices with high video resolution and wide field of view are utilized

Single-Nucleotide Polymorphisms and Markers of Oxidative Stress in Healthy Women

Purpose There is accumulating evidence that oxidative stress is an important contributor to carcinogenesis. We hypothesized that genetic variation in genes involved in maintaining antioxidant/ oxidant balance would be associated with overall oxidative stress. Methods We examined associations between single nucleotide polymorphisms (SNPs) in MnSOD, GSTP1, GSTM1, GPX1, GPX3, and CAT genes and thiobarbituric acid-reactive substances (TBARS), a blood biomarker of oxidative damage, in healthy white women randomly selected from Western New York (n = 1402). We used general linear models to calculate age-adjusted geometric means of TBARS across the variants. We also examined the associations within strata of menopausal status. Results For MnSOD, being heterozygous was associated with lower geometric means of TBARS (less oxidative stress), 1.28 mg/dL, compared to homozygous T-allele or homozygous Callele, 1.35 mg/dL, and 1.31 mg/dL correspondingly (p for trend = 0.01). This difference remained among postmenopausal women, 1.40 mg/dL for TT, 1.32 mg/dL for TC, and 1.34mg/dL for CC (p for trend 0.015); it was attenuated among premenopausal women. SNPs in the other genes examined (GSTP1, GSTM1, GPX1, GPX3, and CAT) were not associated with TBARS. Conclusions Our findings suggest that genetic variation in MnSOD gene may be associated with oxidative status, particularly among postmenopausal women

Elongation rate and average length of amyloid fibrils in solution using isotope-labelled small-angle neutron scattering.

Funder: Boehringer Ingelheim FondsFunder: University of BathWe demonstrate a solution method that allows both elongation rate and average fibril length of assembling amyloid fibrils to be estimated. The approach involves acquisition of real-time neutron scattering data during the initial stages of seeded growth, using contrast matched buffer to make the seeds effectively invisible to neutrons. As deuterated monomers add on to the seeds, the labelled growing ends give rise to scattering patterns that we model as cylinders whose increase in length with time gives an elongation rate. In addition, the absolute intensity of the signal can be used to determine the number of growing ends per unit volume, which in turn provides an estimate of seed length. The number of ends did not change significantly during elongation, demonstrating that any spontaneous or secondary nucleation was not significant compared with growth on the ends of pre-existing fibrils, and in addition providing a method of internal validation for the technique. Our experiments on initial growth of alpha synuclein fibrils using 1.2 mg ml-1 seeds in 2.5 mg ml-1 deuterated monomer at room temperature gave an elongation rate of 6.3 ± 0.5 Å min-1, and an average seed length estimate of 4.2 ± 1.3 μm