Results of the 1985 Excavations at Shiplap House (18AP30), 18 Pinkney Street, Annapolis, Maryland

The Shiplap House, 18AP30, located at 18 Pinkney Street, Annapolis, Maryland, was built ca. 1716. Located within the Historic District of Annapolis, Maryland (see Figures 2, 3, & 4 for site location within the Annapolis Historic District), the Shiplap House lies in immediate proximity to features of local and national importance. Due to its potential as a significant archaeological site, the Shiplap property was selected as one of the locations to be investigated during the 1985 summer Fieldschool in Urban Archaeology, a course offerred by the University of Maryland, College Park, under the direction of Dr. Mark P. Leone

Archaeological Testing at the 193 Main St. Site, 18AP44, Annapolis, Maryland

During the Fall of 1985, Mr. Paul Pearson and associates, owners of 193 Main St., Annapolis, Maryland, approached Historical Annapolis Inc. to perform archaeological testing on this property. Mr. Pearson and associates have proposed the construction of a small shopping and business mall on this plot of land, which presently serves as a parking lot, as well as on the adjacent property which contains the Playhouse Theater. According to two reports produced under the sponsorship of the National Endowment for the Humanities (1971 & 1983), this section of Main St. has been an area of social and political significance since the turn of the 18th century. based on the high probability of finding significant archaeological remains, a six week program of testing was planned in the parking lot. This work plan called for an average crew of four field assistants and one supervisor. Excavations began on December 2, 1985 and ended on Jan 17, 1986. Archaeological remains uncovered within the project area were located, identified, and evaluated for potential significance. Funding for this project was generously provided by Mt. Paul Pearson and associates

Factores de impacto en el rendimiento académico universitario. Un estudio a partir de las percepciones de los estudiantes.

n.d.Vázquez, Claudia; Facultad de Ciencias Económicas y Estadística; Universidad Nacional de Rosario; Argentina

La buena docencia desde la mirada de los estudiantes universitarios

El rendimiento académico se encuentra sobredeterminado por múltiples factores interrelacionados estudiados por diversos autores, destacándose el aporte de Garbanzo Vargas (2007), que agrupó los factores en sociales, personales e institucionales, incluyendo en esta última categoría a la relación entre el estudiante y el profesor. La labor del docente adquiere relevancia como mediador en el aprendizaje por lo que interesa identificar que habilidades y competencias debe reunir un profesor universitario para contribuir a mejorar la performance académica de sus estudiantes. En este estudio, el objetivo es conocer el perfil del profesor que prefieren los estudiantes de la carrera Contador Público (UNR) para contribuir al propio desempeño. El abordaje metodológico de la investigación fue de tipo extensivo, utilizando como herramienta una encuesta que respondieron 546 estudiantes, entre primero y quinto año de la carrera elegida. Como resultado, se observa que demostrar conocimientos prácticos, manejar adecuadamente la transmisión de conocimientos y/o contenidos, la accesibilidad ante preguntas por dudas, el respeto al estudiante, revelar conocimientos teóricos y saber relacionar los temas de la asignatura con la práctica profesional futura, son las cualidades más frecuentemente citadas. Por otro lado se destacan el prestigio del docente y el sentido del humor entre los atributos menos importantesAcademic performance is overdetermined by multiple interrelated factors studied by several authors, highlighting the contribution of Garbanzo Vargas (2007), which brought together the factors in social, personal and institutional, including in the latter category the relationship between student and teacher. The teaching becomes important in mediating learning so interested in identifying what skills and competencies must meet a university professor to help improve the academic performance of their students. In this study, the objective is to know the kind of teacher that students prefer CPA (UNR) race to contribute to the performance itself. The methodological approach of the research was extensive type, using a survey tool that responded 546 students between first and fifth year of their chosen career.Fil: Vázquez, Claudia Mónica - Facultad Ciencias Económicas y Estadística - Universidad Nacional de Rosario - Argentin

Human acute myelogenous leukemia stem cells are rare and heterogeneous when assayed in NOD/SCID/IL2Rγc-deficient mice

Human leukemic stem cells, like other cancer stem cells, are hypothesized to be rare, capable of incomplete differentiation, and restricted to a phenotype associated with early hematopoietic progenitors or stem cells. However, recent work in other types of tumors has challenged the cancer stem cell model. Using a robust model of xenotransplantation based on NOD/SCID/IL2Rγc-deficient mice, we confirmed that human leukemic stem cells, functionally defined by us as SCID leukemia-initiating cells (SL-ICs), are rare in acute myelogenous leukemia (AML). In contrast to previous results, SL-ICs were found among cells expressing lineage markers (i.e., among Lin+ cells), CD38, or CD45RA, all markers associated with normal committed progenitors. Remarkably, each engrafting fraction consistently recapitulated the original phenotypic diversity of the primary AML specimen and contained self-renewing leukemic stem cells, as demonstrated by secondary transplants. While SL-ICs were enriched in the Lin–CD38– fraction compared with the other fractions analyzed, SL-ICs in this fraction represented only one-third of all SL-ICs present in the unfractionated specimen. These results indicate that human AML stem cells are rare and enriched but not restricted to the phenotype associated with normal primitive hematopoietic cells. These results suggest a plasticity of the cancer stem cell phenotype that we believe has not been previously described

Tumor-infiltrating mast cells are associated with resistance to anti-PD-1 therapy.

Anti-PD-1 therapy is used as a front-line treatment for many cancers, but mechanistic insight into this therapy resistance is still lacking. Here we generate a humanized (Hu)-mouse melanoma model by injecting fetal liver-derived CD34+ cells and implanting autologous thymus in immune-deficient NOD-scid IL2Rγnull (NSG) mice. Reconstituted Hu-mice are challenged with HLA-matched melanomas and treated with anti-PD-1, which results in restricted tumor growth but not complete regression. Tumor RNA-seq, multiplexed imaging and immunohistology staining show high expression of chemokines, as well as recruitment of FOXP3+ Treg and mast cells, in selective tumor regions. Reduced HLA-class I expression and CD8+/Granz B+ T cells homeostasis are observed in tumor regions where FOXP3+ Treg and mast cells co-localize, with such features associated with resistance to anti-PD-1 treatment. Combining anti-PD-1 with sunitinib or imatinib results in the depletion of mast cells and complete regression of tumors. Our results thus implicate mast cell depletion for improving the efficacy of anti-PD-1 therapy

Tumor-infiltrating mast cells are associated with resistance to anti-PD-1 therapy.

Anti-PD-1 therapy is used as a front-line treatment for many cancers, but mechanistic insight into this therapy resistance is still lacking. Here we generate a humanized (Hu)-mouse melanoma model by injecting fetal liver-derived CD34+ cells and implanting autologous thymus in immune-deficient NOD-scid IL2Rγnull (NSG) mice. Reconstituted Hu-mice are challenged with HLA-matched melanomas and treated with anti-PD-1, which results in restricted tumor growth but not complete regression. Tumor RNA-seq, multiplexed imaging and immunohistology staining show high expression of chemokines, as well as recruitment of FOXP3+ Treg and mast cells, in selective tumor regions. Reduced HLA-class I expression and CD8+/Granz B+ T cells homeostasis are observed in tumor regions where FOXP3+ Treg and mast cells co-localize, with such features associated with resistance to anti-PD-1 treatment. Combining anti-PD-1 with sunitinib or imatinib results in the depletion of mast cells and complete regression of tumors. Our results thus implicate mast cell depletion for improving the efficacy of anti-PD-1 therapy