Supramolecular Control of the Oxidative Addition as a Way To Improve the Catalytic Efficiency of Pincer-Rhodium (I) Complexes

H-1 NMR studies using a cationic complex with a pyridine-di-imidazolylidene pincer ligand of formula [Rh(CNC)(CO)](+) revealed that this compound showed high binding affinity with coronene in CH2Cl2. The interaction between coronene and the planar Rh-I complex is established by means of & pi;-stacking interactions. This interaction has a strong impact on the electron-donating strength of the pincer CNC ligand, which is increased significantly, as demonstrated by the shifting of the & nu;(CO) stretching bands to lower frequencies. The addition of coronene increases the reaction rate of the nucleophilic attack of methyl iodide on the rhodium (I) pincer complex, and also has a positive effect on the performance of the complex as a catalyst in the cycloisomerization of 4-pentynoic acid. These findings highlight the importance of supramolecular interactions for tuning the reactivity and catalytic activity of square-planar metal complexes.Funding for open access charge: CRUE-Universitat Jaume IWe gratefully acknowledge financial support from the Ministerio de Ciencia e Innovación (PID2021-127862NB-I00), and the Universitat Jaume I (UJI-B2020-01 and UJI-B2021-39). We are grateful to the Serveis Centrals d'Instrumentació Científica (SCIC-UJI) for providing with spectroscopic facilities

Pomace from the wine industry as an additive in the production of traditional sustainable lightweight eco-bricks

This study has been funded by Junta de Andalucía Research Group RNM179 and by Research Projects PPJIB2022-17 and B-RNM-188- UGR20 of the Regional Ministry of University, Research and Innovation of the Junta de Andalucía and FEDER, a way of making Europe. We are grateful to Nigel Walkington for his assistance in revising the English text of the manuscript and to three anonymous reviewers for their helpful suggestions. We also thank Bodegas Navarro (www.bodegasn avarro.es), who supplied the wine pomace, and Ceramica Castillo Siles (www.ceramicacastillosiles.es), who provided the raw materials from Viznar and Guadix. We are also grateful to Junta de Andalucía Research Group HUM629.This research examines fired clay bricks made with waste pomace from the wine industry as an additive in brick production. To this end, we analyse and discuss the chemical, mineralogical, textural and physical-mechanical behaviour of fired bricks made with three concentrations of wine pomace (2.5, 5 and 10 wt%) and at three different firing temperatures (800, 950 and 1100 degrees C) and evaluate their durability to salt crystallization. Variations in colour were also examined. The firing process resulted in the decomposition of phyllosilicates and carbonates, the crystallization of Fe oxides and the appearance of high-temperature Ca- (and Mg-) silicates phases such as gehlenite, wollastonite, anorthite and diopside. The bricks made with added wine pomace had very similar mineralogy to the control samples made without it. The bricks made with added wine pomace were lighter than the control samples and underwent less linear shrinkage during the drying process. Particles in the wine pomace were consumed during firing, leading to the appearance of voids. The bricks made with this additive had higher levels of water absorption and poorer mechanical strength. The greatest colour differences were detected after increasing the amount of waste, which generally resulted in yellower bricks. The increase in firing temperature resulted in an improvement in mechanical resistance regardless of the composition of the bricks. However, bricks fired at 1100 degrees C made without additive are more resistant to damage caused by salts than those made with wine pomace.Junta de Andalucia RNM179Regional Ministry of University, Research and Innovation of the Junta de Andalucia PPJIB2022-17, B-RNM-188- UGR20European Union (EU) Spanish GovernmentFEDERJunta de Andalucía Research Group HUM62

Pathogenicity and molecular detection of Uruguayan isolates of Greeneria uvicola and Cadophora luteo-olivacea associated with grapevine trunk diseases

Species from different fungal genera have been indicated as responsible for the development of trunk diseases of grapevines. Greeneria uvicola is responsible for the bitter rot of Vitis vinifera grape bunchesnear harvest, and can also attack other Vitis species. In Uruguay, G. uvicola was isolated from dead arm affected grapevines and as an endophyte from healthy canes. Cadophora luteo-olivacea is a phialophora-likeascomycete with a wide distribution that was isolated from asymptomatic wood tissues in Vitis and Petridisease-affected nursery plants in Uruguay. Pathogenicity of isolates of both species was evaluated on Vitis vinifera cv. Tannat and Cabernet Sauvignon, and rootstocks SO4 and 3309C. Specific primers were developed for the ITS rDNA region for both species. Number of plants showing discoloration, length of discoloration, number of re-isolations and amplifications confirmed the pathogenicity of G. uvicola isolates. Pathogenicityof the isolate of C. luteo-olivacea obtained from symptomatic tissues is discussed. Specific primers can be usedto detect the presence of these fungi in asymptomatic tissues

Modelos de proceso para ingeniería de explotación de información para pymes: abordaje ágil y abordaje robusto

Las metodologías de desarrollo de proyectos de explotación de información existentes, se centran en el proceso de descubrimiento de patrones de conocimiento en la masa de datos. Sin embargo no existe una visión de proceso que identifique fases, tareas, técnicas de representación y procedimientos de ejecución de la tarea; ni que permitan soportar de forma completa e integrada las actividades de administración y desarrollo de proyectos. Resultados de investigación recientes en el área de metricas de proyecto han posibilitado clasificar un determinado proyecto en microproyecto o proyecto regular en función de los valores asociados a dimensión y carga de trabajo. Por analogía con la Ingeniería de Software, que propone metodologías ágiles para cierto tipo de proyectos y metodologías robustas para otros, esta investigación propone cubrir la vacancia de Modelos de Proceso para Proyectos de Ingeniería de Explotación de Información que integre en fases las técnicas desarrolladas para la administración y desarrollo de proyectos de explotación de información en dos versiones, una orientada a microproyectos y otra orientada a proyectos regulares.Eje: Ingeniería de SoftwareRed de Universidades con Carreras en Informática (RedUNCI

Modelos de proceso para ingeniería de explotación de información para pymes: abordaje ágil y abordaje robusto

Las metodologías de desarrollo de proyectos de explotación de información existentes, se centran en el proceso de descubrimiento de patrones de conocimiento en la masa de datos. Sin embargo no existe una visión de proceso que identifique fases, tareas, técnicas de representación y procedimientos de ejecución de la tarea; ni que permitan soportar de forma completa e integrada las actividades de administración y desarrollo de proyectos. Resultados de investigación recientes en el área de metricas de proyecto han posibilitado clasificar un determinado proyecto en microproyecto o proyecto regular en función de los valores asociados a dimensión y carga de trabajo. Por analogía con la Ingeniería de Software, que propone metodologías ágiles para cierto tipo de proyectos y metodologías robustas para otros, esta investigación propone cubrir la vacancia de Modelos de Proceso para Proyectos de Ingeniería de Explotación de Información que integre en fases las técnicas desarrolladas para la administración y desarrollo de proyectos de explotación de información en dos versiones, una orientada a microproyectos y otra orientada a proyectos regulares.Eje: Ingeniería de SoftwareRed de Universidades con Carreras en Informática (RedUNCI

Modelos de proceso para ingeniería de explotación de información para pymes: abordaje ágil y abordaje robusto

Las metodologías de desarrollo de proyectos de explotación de información existentes, se centran en el proceso de descubrimiento de patrones de conocimiento en la masa de datos. Sin embargo no existe una visión de proceso que identifique fases, tareas, técnicas de representación y procedimientos de ejecución de la tarea; ni que permitan soportar de forma completa e integrada las actividades de administración y desarrollo de proyectos. Resultados de investigación recientes en el área de metricas de proyecto han posibilitado clasificar un determinado proyecto en microproyecto o proyecto regular en función de los valores asociados a dimensión y carga de trabajo. Por analogía con la Ingeniería de Software, que propone metodologías ágiles para cierto tipo de proyectos y metodologías robustas para otros, esta investigación propone cubrir la vacancia de Modelos de Proceso para Proyectos de Ingeniería de Explotación de Información que integre en fases las técnicas desarrolladas para la administración y desarrollo de proyectos de explotación de información en dos versiones, una orientada a microproyectos y otra orientada a proyectos regulares.Eje: Ingeniería de SoftwareRed de Universidades con Carreras en Informática (RedUNCI

Biorefinery of lemon peel waste using cold adapted yeasts from Antarctic and Sub-Antarctic regions

Cold adapted yeasts from soil samples from King George Island and Tierra del Fuego province were evaluated for their potential to produce extracellular pectinases. Pectinolytic yeasts were previously identified by 26S rDNA (D1/D2 domain) sequencing and phylogenetic analyses. Among 103 isolates, only eight showed pectinolytic activity at 20ºC, and only four -strains e9.2, 4.6, 5.9 and 8E- were capable to produce pectinolytic activity at 8ºC. Strain 8E identified as Guehomyces pullulans and the strains e9.2 and 5.9 identified as Cystofilobasidium infirmominiatum and Cryptoccocus adeliensis were selected for enzyme production under submerged fermentation. All the strains were capable to grow in presence of lemon peel. C. adeliensis 5.9 produced the highest enzyme activity at 24 h (4.8 U/ml) while C. infirmominiatum e9.2 and G. pullulans 8E showed considerable activity at 45 h (3.9 U/ml and 2.83 U/ml, respectively). It could be seen that at 10ºC enzyme/s remained active. Besides polygalacturonase (PGase), presence of other pectin-degrading enzymes in the culture supernatants was investigated. None of the strains produce neither pectin or pectate lyase activity nor rhamnogalacturonan hydrolase activity. Regarding pectin esterase activity, it was only produced by G. pullulans (0.022 U/ml). All the strains produced enzymatic pools that showed higher activity against highly esterified pectin than against pectin with 63% methoxyl. This behavior could be attributed to the presence of polymethylgalacturonase activity (PMGase) in its supernatant. β- glucosidase activity was detected in all supernatants. This is the first report on the capacity of these species to produce pectinases. Inulinase activity was detected in G. pullulans and C. infirmominiatum supernatants, while xylanase and cellulase activities were only detected in G. pullulans supernatants.Centro de Investigación y Desarrollo en Fermentaciones Industriale

Biorefinery of lemon peel waste using cold adapted yeasts from Antarctic and Sub-Antarctic regions

Cold adapted yeasts from soil samples from King George Island and Tierra del Fuego province were evaluated for their potential to produce extracellular pectinases. Pectinolytic yeasts were previously identified by 26S rDNA (D1/D2 domain) sequencing and phylogenetic analyses. Among 103 isolates, only eight showed pectinolytic activity at 20ºC, and only four -strains e9.2, 4.6, 5.9 and 8E- were capable to produce pectinolytic activity at 8ºC. Strain 8E identified as Guehomyces pullulans and the strains e9.2 and 5.9 identified as Cystofilobasidium infirmominiatum and Cryptoccocus adeliensis were selected for enzyme production under submerged fermentation. All the strains were capable to grow in presence of lemon peel. C. adeliensis 5.9 produced the highest enzyme activity at 24 h (4.8 U/ml) while C. infirmominiatum e9.2 and G. pullulans 8E showed considerable activity at 45 h (3.9 U/ml and 2.83 U/ml, respectively). It could be seen that at 10ºC enzyme/s remained active. Besides polygalacturonase (PGase), presence of other pectin-degrading enzymes in the culture supernatants was investigated. None of the strains produce neither pectin or pectate lyase activity nor rhamnogalacturonan hydrolase activity. Regarding pectin esterase activity, it was only produced by G. pullulans (0.022 U/ml). All the strains produced enzymatic pools that showed higher activity against highly esterified pectin than against pectin with 63% methoxyl. This behavior could be attributed to the presence of polymethylgalacturonase activity (PMGase) in its supernatant. β- glucosidase activity was detected in all supernatants. This is the first report on the capacity of these species to produce pectinases. Inulinase activity was detected in G. pullulans and C. infirmominiatum supernatants, while xylanase and cellulase activities were only detected in G. pullulans supernatants.Centro de Investigación y Desarrollo en Fermentaciones Industriale

Collagen sequence analysis of the extinct giant ground sloths <i>Lestodon</i> and <i>Megatherium</i>

For over 200 years, fossils of bizarre extinct creatures have been described from the Americas that have ranged from giant ground sloths to the 'native' South American ungulates, groups of mammals that evolved in relative isolation on South America. Ground sloths belong to the South American xenarthrans, a group with modern although morphologically and ecologically very different representatives (anteaters, armadillos and sloths), which has been proposed to be one of the four main eutherian clades. Recently, proteomics analyses of bone collagen have recently been used to yield a molecular phylogeny for a range of mammals including the unusual 'Malagasy aardvark' shown to be most closely related to the afrotherian tenrecs, and the south American ungulates supporting their morphological association with condylarths. However, proteomics results generate partial sequence information that could impact upon the phylogenetic placement that has not been appropriately tested. For comparison, this paper examines the phylogenetic potential of proteomicsbased sequencing through the analysis of collagen extracted from two extinct giant ground sloths, Lestodon and Megatherium. The ground sloths were placed as sister taxa to extant sloths, but with a closer relationship between Lestodon and the extant sloths than the basal Megatherium. These results highlight that proteomics methods could yield plausible phylogenies that share similarities with other methods, but have the potential to be more useful in fossils beyond the limits of ancient DNA survival.Facultad de Ciencias Naturales y Muse