Detection and quantitation of HPV in anogenital and oral tissues and fluids of HIV-positive individuals by real-time PCR

Human papillomaviruses (HPV) remain a serious world health problem due to their association with anogenital and oral cancers and warts. While over 100 HPV types have been identified, only a subset is associated with malignancy. HPV16 and 18 are the most common oncogenic types, while HPV6 and 11 are the most common types responsible for anogenital warts. These four types cause up to 90% of HPV-associated disease. While other quantitative PCR (qPCR) assays can be used to detect oncogenic HPV, there is no single tube assay that distinguishes the most frequent oncogenic types and the most common types found in warts. A qPCR assay was developed that allowed for detection and quantitation of these 4 HPV types

Characterization of Facies and Permeability Patterns in Carbonate Reservoirs Based in Outcrop Analogs

More than 13 billion barrels (Bbbl) of mobile oil and 17 Bbbl of residual oil will remain in San Andres and Grayburg reservoirs at abandonment under current development practices. Through the development and application of new recovery technology, a large part of this resource can be recovered. This report focuses on research for the development and testing of new techniques for improving the recovery of this resource. Outcrop and subsurface geologic and engineering data are utilized to develop new methodologies through the integration of geologic observations and engineering data for improving numerical models that predict reservoir performance more accurately. Extensive regional mapping of the 14-mile by 1,200-foot San Andres outcrop, located on the Algerita Escarpment, Guadalupe Mountains, New Mexico, demonstrates that the San Andres carbonate-ramp complex is composed of multiple depositional sequences that have significant basinward shifts in reservoir-quality facies tracts occurring across sequence boundaries. Detailed geologic and petrophysical mapping of three reservoir-quality facies tracts demonstrates that the fundamental scale of geologic description for reservoir characterization is the parasequence and its component rock-fabric-based facies. Descriptions of cores from the Seminole San Andres Unit illustrate that the parasequence is also the fundamental geologic scale for reservoir mapping in the subsurface.Bureau of Economic Geolog

Characterization of Reservoir Heterogenity in Carbonate-Ramp Systems, San Andres/Grayburg Permian Basin

This report summarizes research carried out by the Bureau of Economic Geology's San Andres/Grayburg Reservoir Characterization Research Laboratory (RCRL) from September 1988 through September 1990. The goal of the RCRL program was to develop advanced approaches to reservoir characterization for improved recovery of the substantial remaining mobile oil in San Andres and Grayburg reservoirs. Emphasis was placed on developing an outcrop analog for San Andres strata that could be used as (1) a guide to interpreting the regional and local geologic framework of the subsurface reservoirs and (2) a data source illustrating the scales and patterns of variability of rock-fabric facies and petrophysical properties, particularly in lateral dimensions, and on scales that cannot be studied during subsurface reservoir characterization. Areas selected for study were the San Andres exposures of the Algerita Escarpment in the northern Guadalupe Mountains and the Seminole San Andres Unit on the northern margin of the Central Basin Platform. The outcrop-analog research was emphasized because it had received little attention before this study by either industry or academe. Reports in this summary involve (1) outcrop and subsurface geological characterization of the Algerita Escarpment San Andres and the Seminole San Andres Unit (Kerans), (2) correlation of detailed outcrop mapping in order to research cored wells at Lawyer Canyon, Algerita Escarpment (Nance), (3) diagenetic/petrographic analysis of selected upper San Andres facies focusing on the origin of moldic porosity (Hovorka), (4) geologic engineering description of the upper San Andres carbonates at Lawyer Canyon and the upper producing interval at Seminole (Lucia), (5) geostatistical analysis of permeability patterns and stochastic-based finite-difference modeling of the upper San Andres parasequence window (Senger and Fogg), and (6) deterministic finite element modeling of the upper San Andres parasequence window (Kasap). Availability of basic data for these studies is summarized in the appendix.Bureau of Economic Geolog

Trunk-pelvis coordination during load carriage running.

Understanding the influence of load carriage on trunk-pelvis coordination and its variability has important functional implications for athletes who need to run with load. The aim of this study was to examine the influence of load carriage on trunk-pelvis coordination in running. Thirty healthy adults performed running while wearing a 20% bodyweight backpack, and without load. Vector coding was used to quantify trunk-pelvis segmental coordination and its variability during the stance phase of running. The four coordination patterns were: 1) anti-phase (segments moving in opposite directions), in-phase (segments moving in same directions), trunk-only phase (only trunk movement), and pelvic-only phase (only pelvic movement). For each plane, the percentage of stance phase spent in a specific coordination pattern was quantified. Coordination variability for each plane was averaged over the stance phase. Mixed effects models were used to analyse the effects of load, adjusted for the covariate of sex, on coordination and its variability. Running with load increased trunk-only coordination in the sagittal plane (P < 0.001), increased anti-phase coordination in the frontal plane (P < 0.001), reduced trunk-only phase coordination in axial rotation (P < 0.001), and increased coordination variability in all three planes (Flexion-Extension: P < 0.001; Lateral flexion: P = 0.03; Axial rotation: P < 0.001). Future studies would benefit from investigating how trunk-pelvis coordination and its variability alters candidate end-point variability indices (e.g. COM displacement), and its functional implications in load carriage running

On-line Courses That Really Work: Designing Engaging, Relevant, and Effective Online Courses

In 1999, Donna Abernathy said, Online learning is not the next big thing; it is the now big thing (Donna J. Abernathy, Training + Development Magazine). Fourteen years later, university professors are still debating the merits of online learning and wondering how to make it relevant and effective. Fortunately, we\u27ve learned a lot about what makes online delivery effective for both students and instructors. Attendees will learn successful strategies/practices that will assist in making online learning a satisfying experience for instructors and students, and in doing so creates effective scholarship of teaching and learning environments. We will share what worked and what didn\u27t as we designed online courses, and address the why behind our successes and failures. We will share student work and student reaction to the online format and climate we have created. Join us for a lively discussion of online course delivery that really works

Measuring permanence of CO2 storage in saline formations: the Frio experiment

Bureau of Economic Geolog

Spatiotemporal Expression of flk-1 in Pulmonary Epithelial Cells during Lung Development

Vascular endothelial growth factor-A (VEGF-A) responsive effects mediated via the receptors fetal liver kinase-1 (flk-1) and fms-like tyrosine kinase (flt-1), are key processes of pulmonary vascular development. Flk-1 has been shown to be involved in early embryonic lung epithelial to endothelial crosstalk and branching morphogenesis. Recent reports suggested a role of VEGF-A in lung epithelial cell function. Based on these observations, we hypothesize that epithelial flk-1 has a unique function in pulmonary development. Thus, the aim of this study is to elucidate spatiotemporal expression of flk-1 during lung development with respect to the epithelial system. Embryonic lungs were screened for flk-1 messenger RNA and protein at daily intervals, including postnatal stages. From Embryonic Day (ED) 12.5 through ED 15.5, flk-1 expression was restricted to the early vascular primitive network, while from ED 16.5 on flk-1 was detectable in the epithelial system and persisted there postnatally. At postnatal stages, flk-1 expression was increasingly restricted to individual cells in the alveolar septa. Isolation and in vitro cultivation of alveolar epithelial cells confirmed flk-1 expression and showed VEGF secretion into the supernatant. To our knowledge, this is the first murine study characterizing epithelial flk-1 expression at different stages throughout lung organogenesis until birth and at postnatal stages. To confirm epithelial flk-1 expression, we performed reporter gene analysis of the flk-1 promoter in vivo. Investigations on transgenic mouse strains, containing either a complete or incomplete flk-1 promoter driving expression of the lacZ reporter gene, suggested differential flk-1 regulation in endothelial and epithelial cells

Hepatitis C care cascade among persons born 1945-1965: 3 medical centers

OBJECTIVES: Effective screening, diagnosis, and treatment are needed to reduce chronic hepatitis C virus (HCV) infection-associated morbidity and mortality. In order to successfully increase HCV treatment, it is necessary to identify and understand gaps in linkage of antibody-positive patients with newly identified HCV to subsequent HCV RNA testing, clinical evaluation, and treatment. STUDY DESIGN: To estimate attainment of HCV care cascade steps among antibody-positive patients with newly identified HCV, we conducted chart reviews of patients with a new positive HCV antibody test at 3 academic medical centers participating in the Birth-Cohort Evaluation to Advance Screening and Testing of Hepatitis C (BEST-C) study. METHODS: We tracked receipt of RNA testing, clinical evaluation, treatment initiation, and treatment completion among individuals born between 1945 and 1965 who were newly diagnosed as HCV antibody-positive between December 2012 and October 2015 at 3 BEST-C centers, predominantly from the participating medical centers\u27 primary care practices and emergency departments. RESULTS: Of the 130 HCV-seropositive individuals identified, 118 (91%) had an RNA or genotype test, 75 (58%) were RNA-positive, 73 (56%) were linked to care, 22 (17% overall; 29% among RNA-positive) started treatment, and 21 (16%; 28% among RNA-positive) completed treatment. CONCLUSIONS: This analysis showed that although linkage to care was largely successful in the target birth cohort, the largest gap in the HCV care cascade was seen in initiating treatment. Greater emphasis on linking patients to clinical evaluation and treatment is necessary in order to achieve the public health benefits promised by birth-cohort testing

Hepatitis C care cascade among persons born 1945-1965: 3 medical centers

OBJECTIVES: Effective screening, diagnosis, and treatment are needed to reduce chronic hepatitis C virus (HCV) infection-associated morbidity and mortality. In order to successfully increase HCV treatment, it is necessary to identify and understand gaps in linkage of antibody-positive patients with newly identified HCV to subsequent HCV RNA testing, clinical evaluation, and treatment. STUDY DESIGN: To estimate attainment of HCV care cascade steps among antibody-positive patients with newly identified HCV, we conducted chart reviews of patients with a new positive HCV antibody test at 3 academic medical centers participating in the Birth-Cohort Evaluation to Advance Screening and Testing of Hepatitis C (BEST-C) study. METHODS: We tracked receipt of RNA testing, clinical evaluation, treatment initiation, and treatment completion among individuals born between 1945 and 1965 who were newly diagnosed as HCV antibody-positive between December 2012 and October 2015 at 3 BEST-C centers, predominantly from the participating medical centers\u27 primary care practices and emergency departments. RESULTS: Of the 130 HCV-seropositive individuals identified, 118 (91%) had an RNA or genotype test, 75 (58%) were RNA-positive, 73 (56%) were linked to care, 22 (17% overall; 29% among RNA-positive) started treatment, and 21 (16%; 28% among RNA-positive) completed treatment. CONCLUSIONS: This analysis showed that although linkage to care was largely successful in the target birth cohort, the largest gap in the HCV care cascade was seen in initiating treatment. Greater emphasis on linking patients to clinical evaluation and treatment is necessary in order to achieve the public health benefits promised by birth-cohort testing