The Ursinus Weekly, September 26, 1974

Fall Forum season opens • Editorial: Dear freshmen • A Guide to better living in Collegeville, Pa. 19426 • Bears 1974 football preview • Harriers upset by Delaware Valleyhttps://digitalcommons.ursinus.edu/weekly/1019/thumbnail.jp

The Ursinus Weekly, February 15, 1973

Girls win first meet in new Elliott Pool • 1973 Arts Festival has much to offer • Student Union to open Monday, February 19 • Editorial: Monday to Thursday • First Semester Dean\u27s List • Film review: The Philosopher king • Meistersingers plan busy Spring series • Mermaids sink Temple • U.C. hosts soccer tourney • Win some; Lose one • Bears un-hooped • Forfeits costly; Bear matmen splithttps://digitalcommons.ursinus.edu/weekly/1096/thumbnail.jp

A retrotransposon 412 insertion within an exon of the Drosophila melanogaster vermilion gene is spliced from the precursor RNA.

Three alleles of the Drosophila melanogaster vermilion (v) gene are suppressed by recessive mutations at the suppressor of sable [su(s)], gene. Previous work has established that these alleles have identical insertions of the 412 retrotransposon in the 5'-untranslated region of the gene. Despite the transposon insertion in an exon, v mutants accumulate trace amounts of apparently wild-type-sized transcripts in a su(s)+ background, and the level of v transcript accumulation is increased by su(s) mutations. Here, we have characterized transcripts from a suppressible v mutant in both su(s)+ and su(s)- backgrounds by S1 nuclease protection experiments and sequence analysis of polymerase chain reaction (PCR) generated cDNA clones. We find that transposon sequences are imprecisely eliminated from v mutant transcripts by splicing at donor and acceptor sites located near the ends of the 412 retrotransposon. Four different 5' donor sites are alternatively spliced to a single 3' acceptor site. The implications of this finding are discussed in relation to possible functions of the su(s)+ gene product

The Ursinus Weekly, October 30, 1975

16 U.C. seniors named to Who\u27s who • Prof. presides • U.C. Founder\u27s Day to honor church • Dr. Isaac Asimov named speaker • Editorial: Ursinus\u27 infirmary is ailing! • Letters to the editor: Soccer regrets; Speaking out; Resident vs. day; An open letter to Richard J. Whatley • Newest P.E. prof • Visitor notes strange customs • Renowned sculptor presents project • Forum review: Lisa A. Richette speaks at Ursinus • Ursinus wins! • We\u27re no. 1! • Between inningshttps://digitalcommons.ursinus.edu/weekly/1044/thumbnail.jp

The Ursinus Weekly, April 3, 1975

Someone old, someone new • Come to Ursinus: Land of the Pennsylvania Dutch • CCC student success seen • Newman Club • The Birds • Free, but show invitations • Editorial: Visitation - time for reality? • Letter to the editor: Breakage reply • Gymnastics • Record review: For earth below, Robin Trower • Wanted: More Lantern contributors • Spring sports special: 1975! • 1975 Ursinus baseball outlook • 1975 track prospectus • Another season, another title! • A.L. spotlight • Interview: Pat Williams • National League previewhttps://digitalcommons.ursinus.edu/weekly/1033/thumbnail.jp

The Ursinus Weekly, October 10, 1974

Gov. candidate Drew Lewis fields questions at U.C. • Achatz discusses news media role at Ursinus forum • Parents\u27 Day events slated • USGA continues action policy • Editorial: Ursinus was a people place • Pages from Ursinus past: Radical changes in store for Ursinus by year 1970! • Pumpkin eater\u27s greenery • Personals • A letter to the Weekly • Institute helps pre-meds abroad • Ursinus student publishes histories • Story leaks out • X-country defeated by King\u27s College • Bears will win Saturday! • Hockey team plans tour of Englandhttps://digitalcommons.ursinus.edu/weekly/1021/thumbnail.jp

The Ursinus Weekly, March 13, 1975

New U.S.G.A. elected • Summer school courses slated • Meistersingers begin annual spring tour • Mrs. Clouser appointed • Ec Club hosts alumni • P.M.P.A.C. views the N.H.I.P. • Dare to learn • Get stung • Letters to the editor • Concert review: Argent - March 1, Widener College • Two organizations organized • Placement offers aid • Growth of the Greeks: Omega Chi\u27s sparkling history • Badminton team continues triumphs • Union square: Do you use your Union? • Before the Fest • Editorial: Looking back and forward! • Intramurals instituted • A long way • Sixers in \u2776?https://digitalcommons.ursinus.edu/weekly/1031/thumbnail.jp

High throughput sequencing in mice: a platform comparison identifies a preponderance of cryptic SNPs

<p>Abstract</p> <p>Background</p> <p>Allelic variation is the cornerstone of genetically determined differences in gene expression, gene product structure, physiology, and behavior. However, allelic variation, particularly cryptic (unknown or not annotated) variation, is problematic for follow up analyses. Polymorphisms result in a high incidence of false positive and false negative results in hybridization based analyses and hinder the identification of the true variation underlying genetically determined differences in physiology and behavior. Given the proliferation of mouse genetic models (e.g., knockout models, selectively bred lines, heterogeneous stocks derived from standard inbred strains and wild mice) and the wealth of gene expression microarray and phenotypic studies using genetic models, the impact of naturally-occurring polymorphisms on these data is critical. With the advent of next-generation, high-throughput sequencing, we are now in a position to determine to what extent polymorphisms are currently cryptic in such models and their impact on downstream analyses.</p> <p>Results</p> <p>We sequenced the two most commonly used inbred mouse strains, DBA/2J and C57BL/6J, across a region of chromosome 1 (171.6 – 174.6 megabases) using two next generation high-throughput sequencing platforms: Applied Biosystems (SOLiD) and Illumina (Genome Analyzer). Using the same templates on both platforms, we compared realignments and single nucleotide polymorphism (SNP) detection with an 80 fold average read depth across platforms and samples. While public datasets currently annotate 4,527 SNPs between the two strains in this interval, thorough high-throughput sequencing identified a total of 11,824 SNPs in the interval, including 7,663 new SNPs. Furthermore, we confirmed 40 missense SNPs and discovered 36 new missense SNPs.</p> <p>Conclusion</p> <p>Comparisons utilizing even two of the best characterized mouse genetic models, DBA/2J and C57BL/6J, indicate that more than half of naturally-occurring SNPs remain cryptic. The magnitude of this problem is compounded when using more divergent or poorly annotated genetic models. This warrants full genomic sequencing of the mouse strains used as genetic models.</p

The Ursinus Weekly, March 1, 1973

New USGA officers initiate programs • Ursinus Union apparently an instant, smash success • 1973 Arts Festival begins Friday, March 2 • Ursinus affiliates with United Student Aid Funds • Editorial: The Union • First Union concert presents blend of folk, blues, jazz • New Ruby to be different from former edition • English department will show Oedipus the King • Ursinus beats West Chester in last minute of play • Bears upset LVC • U.C. volleyball clinic features Canadians • Rambo sets record as intercollegiate champ • Letterhttps://digitalcommons.ursinus.edu/weekly/1098/thumbnail.jp

The Ursinus Weekly, April 10, 1975

How to Succeed is Spring production • New Who\u27s who in religion lists Williamson • S.F.A.R.C. studies U.C. campus issues • Computer careers night a big success • Newman Society sponsors mass • Travelin\u27 VIII concert showcase for talent • Letters to the editor • Alarmed by alarms • Education at Ursinus • Casino Night success: Union production a big hit; Gamblers parley thousands into big prizes • Phils win East, Dodgers win West; Oakland repeats, Yanks win East • Preview of \u2775 Wings • Record review: Song for America, Kansas • Come out: See them!https://digitalcommons.ursinus.edu/weekly/1034/thumbnail.jp