Forest Nursery Practices in the Southern United States

Over the past five decades, researchers in the southern United States have been working with nursery managers to develop ways to reduce the cost of producing seedlings. In this regard, the Southern Forest Nursery Management Cooperative (at Auburn University in Alabama) has helped reduce hand-weeding costs and losses due to nematodes and disease. As a result, nursery managers are able to legally use a variety of registered herbicides and fungicides for use in pine and hardwood seedbeds.  Other changes over the last three decades include a reduction in the number of nurseries growing seedlings, a reduction in the number of seedlings outplanted per ha, an increase in the number of container nurseries, an increase in the average production per nursery, an increase in production by the private sector, growing two or more crops after fumigation, the development of synthetic soil stabilizers, applying polyacrylamide gels to roots and the use of seedling bags and boxes for shipping seedlings

Rosa del Olmo Prize: Introductory Essay

Academic prizes have three problems: they feed an individualist ethos, perpetuate the idea of the ‘marketplace of ideas’ as a fair and even playing field, and build a stereotype of white, Western men as the ultimate knowledge creators. However, prizes can also challenge stereotypes and help democratise knowledge creation by enlarging the visibility of communitarian knowledge creation beyond Western scripts and outside hegemonic masculinities. The International Journal for Crime, Justice, and Social Democracy, committed to cognitive justice, knowledge democratisation, and encouraging voices on the periphery to partake in global academic debate, established the Rosa del Olmo Prize. Seeking to challenge criminological stereotypes about who can create knowledge that contributes to the development of criminology, the Journal honours Venezuelan criminologist Rosa del Olmo (1937-2000) through this award. Rosa symbolises critical, feminist, decolonial criminology working to advance social justice

Sulfur and lime affect soil pH and nutrients in a sandy Pinus taeda nursery

Two pH experiments were conducted at a sandy, bareroot loblolly pine (Pinus taeda L.) nursery in Texas. A sulfur trial (0, 813, 1626, 2439 kg ha-1 of elemental sulfur) was installed to determine if lowering soil pH would result in nutrient toxicity symptoms and affect seedling morphology. Although soil acidity in the sulfur study ranged from pH 3.9 to pH 5.0, none of the treatments resulted in micronutrient toxicity and none affected height growth, root-collar diameter, root mass, shoot mass or the root-mass ratio (root dry mass/total dry mass). Acidifying soil with sulfur increased leaching of calcium, potassium, magnesium, manganese and zinc but there was no effect on seedling morphology. The objective of the liming trial (0, 813, 1626, 3252 kg ha-1 of dolomitic lime) was to determine if increasing alkalinity would result in an iron deficiency and reduce seedling growth. As expected, applying lime increased the calcium and magnesium levels but had no effect on soil levels of iron, phosphorus, potassium, sulfur, zinc and sodium. However, the root-mass ratio was reduced by applications of dolomitic lime (pH ranged from 5.3 to 6.0). Differences in soil properties (i.e. plot location) had a greater effect on seedling morphology than lime applications. Foliage levels of manganese and boron were reduced by the highest rate of lime and sulfur, respectively

H3K4 methyltransferase Set1 is involved in maintenance of ergosterol homeostasis and resistance to Brefeldin A

Set1 is a conserved histone H3 lysine 4 (H3K4) methyltransferase that exists as a multisubunit complex. Although H3K4 methylation is located on many actively transcribed genes, few studies have established a direct connection showing that loss of Set1 and H3K4 methylation results in a phenotype caused by disruption of gene expression. In this study, we determined that cells lacking Set1 or Set1 complex members that disrupt H3K4 methylation have a growth defect when grown in the presence of the antifungal drug Brefeldin A (BFA), indicating that H3K4 methylation is needed for BFA resistance. To determine the role of Set1 in BFA resistance, we discovered that Set1 is important for the expression of genes in the ergosterol biosynthetic pathway, including the rate-limiting enzyme HMG-CoA reductase. Consequently, deletion of SET1 leads to a reduction in HMG-CoA reductase protein and total cellular ergosterol. In addition, the lack of Set1 results in an increase in the expression of DAN1 and PDR11, two genes involved in ergosterol uptake. The increase in expression of uptake genes in set1δ cells allows sterols such as cholesterol and ergosterol to be actively taken up under aerobic conditions. Interestingly, when grown in the presence of ergosterol set1δ cells become resistant to BFA, indicating that proper ergosterol levels are needed for antifungal drug resistance. These data show that H3K4 methylation impacts gene expression and output of a biologically and medically relevant pathway and determines why cells lacking H3K4 methylation have antifungal drug sensitivity

Spatial and temporal dimensions of neighborhood effects on high school graduation

Research into the effects of neighborhood characteristics on children’s behavior has burgeoned in recent years, but these studies have generally adopted a limited conceptualization of the spatial and temporal dimensions of neighborhood effects. We use longitudinal data from the Panel Study of Income Dynamics and techniques of spatial data analysis to examine how both the socioeconomic characteristics of extralocal neighborhoods—neighborhoods surrounding the immediate neighborhood of residence—and the duration of exposure to disadvantaged neighborhoods throughout the childhood life course influence the likelihood of graduating from high school. Among blacks and whites, socioeconomic advantage in the immediate neighborhood increases the likelihood of completing high school, but among whites higher levels of socioeconomic advantage in extralocal neighborhoods decrease high school graduation rates. Extralocal neighborhood advantage suppresses the influence of advantage in the immediate neighborhood so that controlling for extralocal conditions provides stronger support for the neighborhood effects hypothesis than has previously been observed. Exposure to advantaged neighborhoods over the childhood life course exerts a stronger effect than point-in-time measures on high school graduation, and racial differences in exposure to advantaged neighbors over the childhood life course help to suppress a net black advantage in the likelihood of completing high school

Immediate chromatin immunoprecipitation and on-bead quantitative PCR analysis: A versatile and rapid ChIP procedure

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. Genome-wide chromatin immunoprecipitation (ChIP) studies have brought significant insight into the genomic localization of chromatin-associated proteins and histone modifications. The large amount of data generated by these analyses, however, require approaches that enable rapid validation and analysis of biological relevance. Furthermore, there are still protein and modification targets that are difficult to detect using standard ChIP methods. To address these issues, we developed an immediate chromatin immunoprecipitation procedure which we call ZipChip. ZipChip significantly reduces the time and increases sensitivity allowing for rapid screening of multiple loci. Here we describe how ZipChIP enables detection of histone modifications (H3K4 mono- and trimethylation) and two yeast histone demethylases, Jhd2 and Rph1, which were previously difficult to detect using standard methods. Furthermore, we demonstrate the versatility of ZipChIP by analyzing the enrichment of the histone deacetylase Sir2 at heterochromatin in yeast and enrichment of the chromatin remodeler, PICKLE, at euchromatin in Arabidopsis thaliana

The wave of wood : forestry's economic contribution to South Carolina's economy in 2018

The purpose of this study is to update the economic contribution of forestry to South Carolina's economy for 2018 and to show the relative magnitude of forestry's major sectors. This study includes business sectors that have a direct and logical connection to the state's forest resource

Charge-based interaction conserved within histone H3 lysine 4 (H3K4) methyltransferase complexes is needed for protein stability, histone methylation, and gene expression

Histone H3 lysine 4 (H3K4) methyltransferases are conserved from yeast to humans, assemble in multisubunit complexes, and are needed to regulate gene expression. The yeast H3K4 methyltransferase complex, Set1 complex or complex of proteins associated with Set1 (COMPASS), consists of Set1 and conserved Set1-associated proteins: Swd1, Swd2, Swd3, Spp1, Bre2, Sdc1, and Shg1. The removal of the WD40 domain-containing subunits Swd1 and Swd3 leads to a loss of Set1 protein and consequently a complete loss ofH3K4methylation. However, until now, how these WD40 domain-containing proteins interact with Set1 and contribute to the stability of Set1 and H3K4 methylation has not been determined. In this study, we identified small basic and acidic patches that mediate protein interactions between theC terminus of Swd1 and the nSET domain of Set1. Absence of either the basic or acidic patches of Set1 and Swd1, respectively, disrupts the interaction between Set1 and Swd1, diminishes Set1 protein levels, and abolishesH3K4methylation. Moreover, these basic and acidic patches are also important for cell growth, telomere silencing, and gene expression. We also show that the basic and acidic patches of Set1 and Swd1 are conserved in their human counter-parts SET1A/B and RBBP5, respectively, and are needed for the protein interaction between SET1A and RBBP5. Therefore, this charge-based interaction is likely important for maintaining the protein stability of the human SET1A/B methyltransferase complexes so that proper H3K4 methylation, cell growth, and gene expression can also occur in mammals. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc

Metropolitan influences on migration into poor and nonpoor neighborhoods

Data from the Panel Study of Income Dynamics and three decennial U.S. censuses are used to examine the influence of metropolitan-area characteristics on black and white households' propensity to move into poor versus nonpoor neighborhoods. We find that a nontrivial portion of the variance in the odds of moving to a poor rather to a nonpoor neighborhood exists between metropolitan areas. Net of established individual-level predictors of inter-neighborhood migration, black and white households are more likely to move to a poor or extremely poor tract rather than to a nonpoor tract in metropolitan areas containing many poor neighborhoods and a paucity of recently-built housing in nonpoor areas. Blacks are especially likely to move to a poor tract in metropolitan areas characterized by high levels of racial residential segregation and in which poor tracts have a sizeable concentration of blacks. White households are more likely to move to a poor than to a nonpoor tract in metropolitan areas that have comparatively few African Americans