Is a Ban on Non-Competes Supported by Empirical Evidence?

The U.S. Federal Trade Commission (FTC) has proposed a rule to declare virtually all non-compete agreements unfair methods of competition under Section 5 of the FTC Act and therefore, illegal. However, the empirical literature on non-compete agreements cited by the FTC in its Notice for Proposed Rulemaking (“NPRM”) shows mixed results on earnings, job creation, firm formation, entrepreneurship, training, investment, and firm value. Evidence in other current studies also does not support an economy-wide ban. The FTC concludes that the proposed rule would yield net benefits even though by its own admission it lacks the information necessary to conduct a cost-benefit analysis (“CBA”). The agency says alternatives to non-competes—such as non-disclosure agreements and nonsolicitation agreements—are comparably effective in protecting investments, but research on this question is virtually non-existent. This Article argues that the FTC’s CBA in its NPRM is flawed and incomplete—assuming away uncertainty, ignoring costs, and failing to show that earnings effects are real, not transfers. Then, this Article proposes that—instead of implementing an economy-wide ban—regulators should focus on more targeted inquiries in industries or occupations where evidence is more conclusive, such as those involving low-wage workers

Neutrophil Isolation Protocol

Neutrophil polymorphonuclear granulocytes (PMN) are the most abundant leukocytes in humans and among the first cells to arrive on the site of inflammatory immune response. Due to their key role in inflammation, neutrophil functions such as locomotion, cytokine production, phagocytosis, and tumor cell combat are extensively studied. To characterize the specific functions of neutrophils, a clean, fast, and reliable method of separating them from other blood cells is desirable for in vitro studies, especially since neutrophils are short-lived and should be used within 2-4 hours of collection. Here, we demonstrate a standard density gradient separation method to isolate human neutrophils from whole blood using commercially available separation media that is a mixture of sodium metrizoate and Dextran 500. The procedure consists of layering whole blood over the density gradient medium, centrifugation, separation of neutrophil layer, and lysis of residual erythrocytes. Cells are then washed, counted, and resuspended in buffer to desired concentration. When performed correctly, this method has been shown to yield samples of >95% neutrophils with >95% viability

14-01 Exploring the Equity Dimensions of US Bicycle Sharing Systems

Research over the past several decades has made it increasingly clear that livable communities are inextricably linked with the provision of opportunities for active and/or non-motorized transportation; i.e., walking, cycling and their variants. An emerging phenomena that is working within the broader movement of active transportation is public bicycle sharing systems (BSS). Such systems have grown considerably in the US in recent years and, in some cases, are dramatically changing the ecology of urban transport. Alongside celebrations of the early successes of US BSS, have been criticisms that these systems have not been adequately integrated into lower-income communities; a pattern that mirrors (motorized) transportation injustices-both past and present-that have burdened lower-income while simultaneously advantaging middle to higher-income communities. And while diverse communities are embracing non-motorized transportation, there is valid concern that traditionally underserved populations will again be marginalized or unable to share in the full benefits of existing and future bicycle- and pedestrian-oriented infrastructure including BSS. This research explores the spatial arrangements and allocations of US BSS and examines the extent to which lower-income communities experience differential access to bike-sharing infrastructure. Spatial regression models are employed to examine the degree to which race, ethnicity and/or economic hardship explain variations in the distribution of bike-sharing stations

Endobronchial ultrasound-guided intranodal forceps biopsy (EBUS-IFB)-technical review

Endobronchial ultrasound (EBUS) and transbronchial needle aspiration (TBNA) have changed the landscape of pulmonology. Mediastinal structures beyond the confines of airway walls are visualized in real-time with EBUS, leading to improved accuracy of tissue sampling and diagnostic yield. With the development of various needle sizes ranging from 25-G to 19-G, the sampling of lymph nodes is becoming easier and more commonplace. Yet, certain conditions such as sarcoidosis and lymphoma may still be difficult to diagnose via EBUS-TBNA. Furthermore, in the age of targeted therapy, there are more demands on EBUS-TBNA samples for molecular marker testing and next-generation sequencing. Here, we present a complementary methodology, EBUS-guided intranodal forceps biopsy (EBUS-IFB), for tissue acquisition that may help address these deficiencies. Specifically, we aim to propose indications, contraindications, outline approaches in performing IFB, and provide an overview of the data for this complementary technique

15-02 Estimating and Enhancing Public Transit Accessibility for People with Mobility Limitations

This two-part study employs fine-scale performance measures and analytical techniques designed to evaluate and improve transit services for people experiencing disability. Part one puts forth a series of time-sensitive, general transit feed system (GTFS)-enhanced employment accessibility models that account for multiple transportation modes, categories of functional limitation and design characteristics of existing public transit infrastructure. Model results shed light on the degree to which a medium-size city’s public transit system addresses the gap between a theoretical continuum of rider capacities and the physical demands required to achieve mobility and access to employment. Our research finds that an individual’s combined physical mobility constraints (e.g., walking speed and maximum walking distance) and public transit infrastructure requirements (e.g., presence/absence of wheelchair boarding platforms and connections to pedestrian access routes) may reduce employment accessibility outcomes by as much as 86 percent. Part two of the study utilizes performance measures developed in part one to model—via spatially explicit structural equations—the degree to which employment accessibility explains variations in public transit ridership and work commute transportation mode share. Here we find that commute share and ridership…(results). Developing a better understanding of relationships between accessibility and transit usage, we reason, will help shed light on how American Disabilities Act (ADA) compliant transit infrastructure affects mode choice decisions among people with considerable functional limitations and across the broader population

Inflammatory pathophysiology as a contributor to myeloproliferative neoplasms

Myeloid neoplasms, including acute myeloid leukemia (AML), myeloproliferative neoplasms (MPNs), and myelodysplastic syndromes (MDS), feature clonal dominance and remodeling of the bone marrow niche in a manner that promotes malignant over non-malignant hematopoiesis. This take-over of hematopoiesis by the malignant clone is hypothesized to include hyperactivation of inflammatory signaling and overproduction of inflammatory cytokines. In th

Peptidylarginine deiminase (PAD) is a mouse cortical granule protein that plays a role in preimplantation embryonic development

BACKGROUND: While mammalian cortical granules are important in fertilization, their biochemical composition and functions are not fully understood. We previously showed that the ABL2 antibody, made against zona free mouse blastocysts, binds to a 75-kDa cortical granule protein (p75) present in a subpopulation of mouse cortical granules. The purpose of this study was to identify and characterize p75, examine its distribution in unfertilized oocytes and preimplantation embryos, and investigate its biological role in fertilization. RESULTS: To identify p75, the protein was immunoprecipitated from ovarian lysates with the ABL2 antibody and analyzed by tandem mass spectrometry (MS/MS). A partial amino acid sequence (VLIGGSFY) was obtained, searched against the NCBI nonredundant database using two independent programs, and matched to mouse peptidylarginine deiminase (PAD). When PAD antibody was used to probe western blots of p75, the antibody detected a single protein band with a molecular weight of 75 kDa, confirming our mass spectrometric identification of p75. Immunohistochemistry demonstrated that PAD was present in the cortical granules of unfertilized oocytes and was released from activated and in vivo fertilized oocytes. After its release, PAD was observed in the perivitelline space, and some PAD remained associated with the oolemma and blastomeres' plasma membranes as a peripheral membrane protein until the blastocyst stage of development. In vitro treatment of 2-cell embryos with the ABL2 antibody or a PAD specific antibody retarded preimplantation development, suggesting that cortical granule PAD plays a role after its release in preimplantation cleavage and early embryonic development. CONCLUSION: Our data showed that PAD is present in the cortical granules of mouse oocytes, is released extracellularly during the cortical reaction, and remains associated with the blastomeres' surfaces as a peripheral membrane protein until the blastocyst stage of development. Our in vitro study supports the idea that extracellular PAD functions in preimplantation development

CubeSat Radiation Hardness Assurance Beyond Total Dose: Evaluating Single Event Effects

Radiation poses known and serious risks to smallsat survivability and mission duration, with effects falling into two categories: long-term total ionizing dose (TID) and instantaneous single event effects (SEE). Although literature exists on the topic of addressing TID in smallsats, few resources exist for addressing SEEs. Many varieties of SEEs exist, such as bit upsets and latch ups, which can occur in any electronic component containing active semiconductors (such as transistors). SEE consequences range from benign to destructive, so mission reliability can be enhanced by implementing fault protection strategies based on predicted SEE rates. Unfortunately, SEE rates are most reliably estimated through experimental testing that is often too costly for smallsat-scale missions. Prior test data published by larger programs exist, but may be sparse or incompatible with the environment of a particular mission. Despite these limitations, a process may be followed to gain insights and make informed design decisions for smallsats in the absence of hardware testing capabilities or similar test data. This process is: (1) Define the radiation environment; (2) identify the most critical and/or susceptible components on a spacecraft; (3) perform a search for compatible prior test data and/or component class data; (4) evaluate mission-specific SEE rates from available data; (5) study the rates alongside the mission requirements to identify high-risk areas of potential mitigation. The methodology developed in this work is based on the multi-institutional, National Science Foundation (NSF) Space Weather Atmospheric Reconfigurable Multiscale Experiment (SWARM-EX) mission. The steps taken during SWARM-EX’s radiation analysis alongside the detailed methodology serve as a case study for how these techniques can be applied to increasing the reliability of a university-scale smallsat mission