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Purification and Characterization of Ascaris Suum Hexokinase
Hexokinase has been found in a soluble and a bound form in homogenates of Ascaris suum muscle. Cellulose acetate electrophoresis, isoelectric focusing, and ion exchange chromatography confirmed the presence of only one molecular form of hexokinase in this muscle. A procedure for purifying hexokinase from Ascaris muscle has been developed. The physical and catalytic properties of the enzyme are comparable to mammalian hexokinases. The enzyme is a monomer with a molecular weight of 100,000 as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and gel filtration. The Michaelis constants for glucose and ATP are 4.7 x 10-3 M and 2.2 x 10-4 M, respectively. Ascaris' hexokinase also exhibits end-product inhibition by glucose 6-phosphate and ADP. It is postulated that the kinetic para meters of the enzyme are the results of its function, that of generating glucose 6-phosphate primarily for glycogen synthesis